Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence

Autores
Palma, Leopoldo; Ortiz, Leila Melina; Niz, José María; Berretta, Marcelo Facundo; Sauka, Diego Hernan
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina.
Fil: Palma, Leopoldo. Universitat de València, Departamento de Genética. Instituto BIOTECMED, Laboratorio de Control Biotecnológico de Plagas; España
Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Data 9 (3) : 40 (February 2024)
Materia
Control Biológico
Plagas
Insecticidas
Pests
Biological Control
Insecticides
Bacillus thuringiensis
Biovar Thuringiensis
Insecticidal Proteins
Genome Sequence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/20529
Acceder
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oai_identifier_str oai:localhost:20.500.12123/20529
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic SequencePalma, LeopoldoOrtiz, Leila MelinaNiz, José MaríaBerretta, Marcelo FacundoSauka, Diego HernanControl BiológicoPlagasInsecticidasPestsBiological ControlInsecticidesBacillus thuringiensisBiovar ThuringiensisInsecticidal ProteinsGenome SequenceThe genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina.Fil: Palma, Leopoldo. Universitat de València, Departamento de Genética. Instituto BIOTECMED, Laboratorio de Control Biotecnológico de Plagas; EspañaFil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2024-12-09T14:30:21Z2024-12-09T14:30:21Z2024-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/20529https://www.mdpi.com/2306-5729/9/3/402306-5729https://doi.org/10.3390/data9030040Data 9 (3) : 40 (February 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestalesinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:47Zoai:localhost:20.500.12123/20529instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:48.247INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
title Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
spellingShingle Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
Palma, Leopoldo
Control Biológico
Plagas
Insecticidas
Pests
Biological Control
Insecticides
Bacillus thuringiensis
Biovar Thuringiensis
Insecticidal Proteins
Genome Sequence
title_short Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
title_full Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
title_fullStr Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
title_full_unstemmed Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
title_sort Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
dc.creator.none.fl_str_mv Palma, Leopoldo
Ortiz, Leila Melina
Niz, José María
Berretta, Marcelo Facundo
Sauka, Diego Hernan
author Palma, Leopoldo
author_facet Palma, Leopoldo
Ortiz, Leila Melina
Niz, José María
Berretta, Marcelo Facundo
Sauka, Diego Hernan
author_role author
author2 Ortiz, Leila Melina
Niz, José María
Berretta, Marcelo Facundo
Sauka, Diego Hernan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Control Biológico
Plagas
Insecticidas
Pests
Biological Control
Insecticides
Bacillus thuringiensis
Biovar Thuringiensis
Insecticidal Proteins
Genome Sequence
topic Control Biológico
Plagas
Insecticidas
Pests
Biological Control
Insecticides
Bacillus thuringiensis
Biovar Thuringiensis
Insecticidal Proteins
Genome Sequence
dc.description.none.fl_txt_mv The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina.
Fil: Palma, Leopoldo. Universitat de València, Departamento de Genética. Instituto BIOTECMED, Laboratorio de Control Biotecnológico de Plagas; España
Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-09T14:30:21Z
2024-12-09T14:30:21Z
2024-02-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/20529
https://www.mdpi.com/2306-5729/9/3/40
2306-5729
https://doi.org/10.3390/data9030040
url http://hdl.handle.net/20.500.12123/20529
https://www.mdpi.com/2306-5729/9/3/40
https://doi.org/10.3390/data9030040
identifier_str_mv 2306-5729
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Data 9 (3) : 40 (February 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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