Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina

Autores
Arneodo Larochette, Joel Demian; De Anna, J.; Salvador, Ricardo; Farinon, Omar Marcelo; Quintana, Graciela Mabel; Sciocco, Alicia Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3–50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef‐8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well‐defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV‐M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8–21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.
Instituto de Microbiología y Zoología Agrícola
Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: De Anna, J. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Farinon, Omar Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Quintana, Graciela Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fuente
Annals of applied biology 166 (1) : 67-74. (January 2015)
Materia
Cydia Pomonella
Lepidoptera
Baculoviridae
Diversidad Genética como Recurso
Virus de las Plantas
Genetic Diversity as Resource
Plant Viruses
Granulovirus
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2233
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spelling Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in ArgentinaArneodo Larochette, Joel DemianDe Anna, J.Salvador, RicardoFarinon, Omar MarceloQuintana, Graciela MabelSciocco, Alicia InesCydia PomonellaLepidopteraBaculoviridaeDiversidad Genética como RecursoVirus de las PlantasGenetic Diversity as ResourcePlant VirusesGranulovirusArgentinaThe occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3–50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef‐8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well‐defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV‐M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8–21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.Instituto de Microbiología y Zoología AgrícolaFil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: De Anna, J. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Farinon, Omar Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Quintana, Graciela Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina2018-04-12T16:58:18Z2018-04-12T16:58:18Z2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12162http://hdl.handle.net/20.500.12123/22330003-47461744-7348https://doi.org/10.1111/aab.12162Annals of applied biology 166 (1) : 67-74. (January 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-10-23T11:16:30Zoai:localhost:20.500.12123/2233instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:30.755INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
spellingShingle Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
Arneodo Larochette, Joel Demian
Cydia Pomonella
Lepidoptera
Baculoviridae
Diversidad Genética como Recurso
Virus de las Plantas
Genetic Diversity as Resource
Plant Viruses
Granulovirus
Argentina
title_short Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_full Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_fullStr Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_full_unstemmed Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_sort Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
dc.creator.none.fl_str_mv Arneodo Larochette, Joel Demian
De Anna, J.
Salvador, Ricardo
Farinon, Omar Marcelo
Quintana, Graciela Mabel
Sciocco, Alicia Ines
author Arneodo Larochette, Joel Demian
author_facet Arneodo Larochette, Joel Demian
De Anna, J.
Salvador, Ricardo
Farinon, Omar Marcelo
Quintana, Graciela Mabel
Sciocco, Alicia Ines
author_role author
author2 De Anna, J.
Salvador, Ricardo
Farinon, Omar Marcelo
Quintana, Graciela Mabel
Sciocco, Alicia Ines
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cydia Pomonella
Lepidoptera
Baculoviridae
Diversidad Genética como Recurso
Virus de las Plantas
Genetic Diversity as Resource
Plant Viruses
Granulovirus
Argentina
topic Cydia Pomonella
Lepidoptera
Baculoviridae
Diversidad Genética como Recurso
Virus de las Plantas
Genetic Diversity as Resource
Plant Viruses
Granulovirus
Argentina
dc.description.none.fl_txt_mv The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3–50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef‐8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well‐defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV‐M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8–21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.
Instituto de Microbiología y Zoología Agrícola
Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: De Anna, J. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Farinon, Omar Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Quintana, Graciela Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
description The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3–50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef‐8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well‐defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV‐M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8–21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
2018-04-12T16:58:18Z
2018-04-12T16:58:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12162
http://hdl.handle.net/20.500.12123/2233
0003-4746
1744-7348
https://doi.org/10.1111/aab.12162
url https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12162
http://hdl.handle.net/20.500.12123/2233
https://doi.org/10.1111/aab.12162
identifier_str_mv 0003-4746
1744-7348
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Annals of applied biology 166 (1) : 67-74. (January 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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