Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina

Autores
Arneodo Larochette, Joel Demián; De Anna, J.; Salvador, R.; Farinon, M.; Quintana, G.; Sciocco Cap, A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV-based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3-50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef-8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well-defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV-M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8-21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.
Fil: Arneodo Larochette, Joel Demián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Anna, J.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Salvador, R.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Farinon, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Quintana, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sciocco Cap, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Materia
Baculoviridae
Cpgv
Cydia Pomonella
Epidemiology
Genetic Diversity
Lepidoptera
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37395
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37395
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in ArgentinaArneodo Larochette, Joel DemiánDe Anna, J.Salvador, R.Farinon, M.Quintana, G.Sciocco Cap, A.BaculoviridaeCpgvCydia PomonellaEpidemiologyGenetic DiversityLepidopterahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV-based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3-50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef-8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well-defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV-M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8-21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.Fil: Arneodo Larochette, Joel Demián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Anna, J.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Salvador, R.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Farinon, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Quintana, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Sciocco Cap, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaWiley2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37395Arneodo Larochette, Joel Demián; De Anna, J.; Salvador, R.; Farinon, M.; Quintana, G.; et al.; Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina; Wiley; Annals of Applied Biology; 166; 1; 1-2015; 67-740003-4746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/aab.12162info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:26Zoai:ri.conicet.gov.ar:11336/37395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:26.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
spellingShingle Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
Arneodo Larochette, Joel Demián
Baculoviridae
Cpgv
Cydia Pomonella
Epidemiology
Genetic Diversity
Lepidoptera
title_short Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_full Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_fullStr Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_full_unstemmed Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
title_sort Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
dc.creator.none.fl_str_mv Arneodo Larochette, Joel Demián
De Anna, J.
Salvador, R.
Farinon, M.
Quintana, G.
Sciocco Cap, A.
author Arneodo Larochette, Joel Demián
author_facet Arneodo Larochette, Joel Demián
De Anna, J.
Salvador, R.
Farinon, M.
Quintana, G.
Sciocco Cap, A.
author_role author
author2 De Anna, J.
Salvador, R.
Farinon, M.
Quintana, G.
Sciocco Cap, A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Baculoviridae
Cpgv
Cydia Pomonella
Epidemiology
Genetic Diversity
Lepidoptera
topic Baculoviridae
Cpgv
Cydia Pomonella
Epidemiology
Genetic Diversity
Lepidoptera
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV-based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3-50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef-8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well-defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV-M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8-21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.
Fil: Arneodo Larochette, Joel Demián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Anna, J.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Salvador, R.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Farinon, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Quintana, G.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sciocco Cap, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
description The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV-based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3-50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef-8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well-defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV-M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8-21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37395
Arneodo Larochette, Joel Demián; De Anna, J.; Salvador, R.; Farinon, M.; Quintana, G.; et al.; Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina; Wiley; Annals of Applied Biology; 166; 1; 1-2015; 67-74
0003-4746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37395
identifier_str_mv Arneodo Larochette, Joel Demián; De Anna, J.; Salvador, R.; Farinon, M.; Quintana, G.; et al.; Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina; Wiley; Annals of Applied Biology; 166; 1; 1-2015; 67-74
0003-4746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/aab.12162
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.229304

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