Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats

Autores
Iacoboni, Paola Anabella; Hasenauer, Flavia Carolina; Caffaro, Maria Eugenia; Gaido, Analía Beatriz; Rossetto, Cristina; Neumann, Roberto; Salatin, Antonio; Bertoni, Emiliano; Poli, Mario Andres; Rossetti, Carlos Alberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.
Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Hasenauer, Flavia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Caffaro, Maria Eugenia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto. Agencia de Extension Rural Oran.; Argentina
Fil: Neumann, Roberto. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Salatin, Antonio. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Bertoni, Emiliano. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Materia
SLC11A1 GENE
GOATS
BRUCELLOSIS
INNATE RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103758
Acceder
id CONICETDig_07ae225e2d93fecb81bd91b95a524393
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goatsIacoboni, Paola AnabellaHasenauer, Flavia CarolinaCaffaro, Maria EugeniaGaido, Analía BeatrizRossetto, CristinaNeumann, RobertoSalatin, AntonioBertoni, EmilianoPoli, Mario AndresRossetti, Carlos AlbertoSLC11A1 GENEGOATSBRUCELLOSISINNATE RESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Hasenauer, Flavia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Caffaro, Maria Eugenia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; ArgentinaFil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto. Agencia de Extension Rural Oran.; ArgentinaFil: Neumann, Roberto. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; ArgentinaFil: Salatin, Antonio. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; ArgentinaFil: Bertoni, Emiliano. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; ArgentinaFil: Poli, Mario Andres. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaElsevier Science2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103758Iacoboni, Paola Anabella; Hasenauer, Flavia Carolina; Caffaro, Maria Eugenia; Gaido, Analía Beatriz; Rossetto, Cristina; et al.; Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats; Elsevier Science; Veterinary Immunology And Immunopathology; 160; 3-4; 8-2014; 230-2340165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165242714001202info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2014.05.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:16Zoai:ri.conicet.gov.ar:11336/103758instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:16.44CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
spellingShingle Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
Iacoboni, Paola Anabella
SLC11A1 GENE
GOATS
BRUCELLOSIS
INNATE RESISTANCE
title_short Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_full Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_fullStr Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_full_unstemmed Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_sort Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
dc.creator.none.fl_str_mv Iacoboni, Paola Anabella
Hasenauer, Flavia Carolina
Caffaro, Maria Eugenia
Gaido, Analía Beatriz
Rossetto, Cristina
Neumann, Roberto
Salatin, Antonio
Bertoni, Emiliano
Poli, Mario Andres
Rossetti, Carlos Alberto
author Iacoboni, Paola Anabella
author_facet Iacoboni, Paola Anabella
Hasenauer, Flavia Carolina
Caffaro, Maria Eugenia
Gaido, Analía Beatriz
Rossetto, Cristina
Neumann, Roberto
Salatin, Antonio
Bertoni, Emiliano
Poli, Mario Andres
Rossetti, Carlos Alberto
author_role author
author2 Hasenauer, Flavia Carolina
Caffaro, Maria Eugenia
Gaido, Analía Beatriz
Rossetto, Cristina
Neumann, Roberto
Salatin, Antonio
Bertoni, Emiliano
Poli, Mario Andres
Rossetti, Carlos Alberto
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SLC11A1 GENE
GOATS
BRUCELLOSIS
INNATE RESISTANCE
topic SLC11A1 GENE
GOATS
BRUCELLOSIS
INNATE RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.
Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Hasenauer, Flavia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Caffaro, Maria Eugenia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto. Agencia de Extension Rural Oran.; Argentina
Fil: Neumann, Roberto. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Salatin, Antonio. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Bertoni, Emiliano. Instituto Nacional de Tecnología Agropecuaria; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Salta. Agencia de Extension Rural Cafayate.; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
description Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103758
Iacoboni, Paola Anabella; Hasenauer, Flavia Carolina; Caffaro, Maria Eugenia; Gaido, Analía Beatriz; Rossetto, Cristina; et al.; Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats; Elsevier Science; Veterinary Immunology And Immunopathology; 160; 3-4; 8-2014; 230-234
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103758
identifier_str_mv Iacoboni, Paola Anabella; Hasenauer, Flavia Carolina; Caffaro, Maria Eugenia; Gaido, Analía Beatriz; Rossetto, Cristina; et al.; Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats; Elsevier Science; Veterinary Immunology And Immunopathology; 160; 3-4; 8-2014; 230-234
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165242714001202
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2014.05.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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