Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resi...

Autores
Ferreira, Flavia Vanina; Herrmann Andrade, Andreina M.; Calabrese, Carla D.; Bello, Fernando; Vazquez, Daniel Eduardo; Musumeci, Matías Alejandro
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Aims: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporiodies are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. Methods and Results: Seven strains were isolated and identified as Trichoderma harzianum, T. ghizhouense, T. atroviride and T. koningiopsis through morphological and molecular analysis. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC‐30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80 % after three weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activity. Conclusions: T. harzianum IC‐30 is an optimal antagonist for the control of green mold spreading and other pathogens in post‐harvest citrus fruits. Significance and impact: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides.
EEA Concordia
Fil: Ferreira, Flavia V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina
Fil: Herrmann Andrade, Andreina M. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Calabrese, Carla D. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Musumeci, Matías A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y TranUniversidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentinasferencia de Entre Ríos; Argentina.
Fuente
Journal of Applied Microbiology Accepted Articles (April 6, 2020)
Materia
Citrus
Trichoderma
Naranja Dulce
Enfermedades de las Plantas
Control de Enfermedades
Penicillium digitatum
Alternaria alternata
Colletotrichum
Sweet Oranges
Plant Diseases
Diseases Control
Colletotrichum gloeosporiodies
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7058
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oai_identifier_str oai:localhost:20.500.12123/7058
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spelling Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]Ferreira, Flavia VaninaHerrmann Andrade, Andreina M.Calabrese, Carla D.Bello, FernandoVazquez, Daniel EduardoMusumeci, Matías AlejandroCitrusTrichodermaNaranja DulceEnfermedades de las PlantasControl de EnfermedadesPenicillium digitatumAlternaria alternataColletotrichumSweet OrangesPlant DiseasesDiseases ControlColletotrichum gloeosporiodiesAims: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporiodies are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. Methods and Results: Seven strains were isolated and identified as Trichoderma harzianum, T. ghizhouense, T. atroviride and T. koningiopsis through morphological and molecular analysis. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC‐30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80 % after three weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activity. Conclusions: T. harzianum IC‐30 is an optimal antagonist for the control of green mold spreading and other pathogens in post‐harvest citrus fruits. Significance and impact: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides.EEA ConcordiaFil: Ferreira, Flavia V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos; ArgentinaFil: Herrmann Andrade, Andreina M. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Calabrese, Carla D. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Musumeci, Matías A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y TranUniversidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentinasferencia de Entre Ríos; Argentina.Wileyinfo:eu-repo/date/embargoEnd/2021-04-082020-04-08T13:06:24Z2020-04-08T13:06:24Z2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7058https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.146571364-50721365-2672https://doi.org/10.1111/jam.14657Journal of Applied Microbiology Accepted Articles (April 6, 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:07:53Zoai:localhost:20.500.12123/7058instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:53.597INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
title Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
spellingShingle Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
Ferreira, Flavia Vanina
Citrus
Trichoderma
Naranja Dulce
Enfermedades de las Plantas
Control de Enfermedades
Penicillium digitatum
Alternaria alternata
Colletotrichum
Sweet Oranges
Plant Diseases
Diseases Control
Colletotrichum gloeosporiodies
title_short Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
title_full Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
title_fullStr Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
title_full_unstemmed Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
title_sort Effectiveness of Trichoderma strains isolated from the rhizosphere of citrus tree to control Alternaria alternata, Colletotrichum gloeosporioides and Penicillium digitatum A21 resistant to pyrimethanil in post‐harvest oranges [Citrus sinensis L. (Osbeck)]
dc.creator.none.fl_str_mv Ferreira, Flavia Vanina
Herrmann Andrade, Andreina M.
Calabrese, Carla D.
Bello, Fernando
Vazquez, Daniel Eduardo
Musumeci, Matías Alejandro
author Ferreira, Flavia Vanina
author_facet Ferreira, Flavia Vanina
Herrmann Andrade, Andreina M.
Calabrese, Carla D.
Bello, Fernando
Vazquez, Daniel Eduardo
Musumeci, Matías Alejandro
author_role author
author2 Herrmann Andrade, Andreina M.
Calabrese, Carla D.
Bello, Fernando
Vazquez, Daniel Eduardo
Musumeci, Matías Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Citrus
Trichoderma
Naranja Dulce
Enfermedades de las Plantas
Control de Enfermedades
Penicillium digitatum
Alternaria alternata
Colletotrichum
Sweet Oranges
Plant Diseases
Diseases Control
Colletotrichum gloeosporiodies
topic Citrus
Trichoderma
Naranja Dulce
Enfermedades de las Plantas
Control de Enfermedades
Penicillium digitatum
Alternaria alternata
Colletotrichum
Sweet Oranges
Plant Diseases
Diseases Control
Colletotrichum gloeosporiodies
dc.description.none.fl_txt_mv Aims: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporiodies are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. Methods and Results: Seven strains were isolated and identified as Trichoderma harzianum, T. ghizhouense, T. atroviride and T. koningiopsis through morphological and molecular analysis. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC‐30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80 % after three weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activity. Conclusions: T. harzianum IC‐30 is an optimal antagonist for the control of green mold spreading and other pathogens in post‐harvest citrus fruits. Significance and impact: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides.
EEA Concordia
Fil: Ferreira, Flavia V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina
Fil: Herrmann Andrade, Andreina M. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Calabrese, Carla D. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Musumeci, Matías A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y TranUniversidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentinasferencia de Entre Ríos; Argentina.
description Aims: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporiodies are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. Methods and Results: Seven strains were isolated and identified as Trichoderma harzianum, T. ghizhouense, T. atroviride and T. koningiopsis through morphological and molecular analysis. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC‐30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80 % after three weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activity. Conclusions: T. harzianum IC‐30 is an optimal antagonist for the control of green mold spreading and other pathogens in post‐harvest citrus fruits. Significance and impact: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-08T13:06:24Z
2020-04-08T13:06:24Z
2020-04
info:eu-repo/date/embargoEnd/2021-04-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7058
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14657
1364-5072
1365-2672
https://doi.org/10.1111/jam.14657
url http://hdl.handle.net/20.500.12123/7058
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14657
https://doi.org/10.1111/jam.14657
identifier_str_mv 1364-5072
1365-2672
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Applied Microbiology Accepted Articles (April 6, 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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