Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine

Autores
Scialfa, Exequiel; Videla, Yanina; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; Brihuega, Bibiana Felicitas
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
Instituto de Patobiología
Fil: Scialfa, Exequiel. Ministerio de Salud. División Zoonosis Rurales; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Videla, Yanina. Ministerio de Salud. División Zoonosis Rurales; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Quintana, Silvina. Instituto de Análisis Fares Taie; Argentina
Fil: Aguirre, Pablo. Ministerio de Salud. División Zoonosis Rurales; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fuente
Journal of Microbiology, Biotechnology and Food Sciences 8 (6) : 1265-1270 (Julio 2019)
Materia
Leptospira
Aislamiento
Argentina
Isolation
Saprophytism
Saprofitismo
Aquaspirillum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5782
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5782
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spelling Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of ArgentineScialfa, ExequielVidela, YaninaGrune Loffler, SylviaQuintana, SilvinaAguirre, PabloBrihuega, Bibiana FelicitasLeptospiraAislamientoArgentinaIsolationSaprophytismSaprofitismoAquaspirillumLeptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.Instituto de PatobiologíaFil: Scialfa, Exequiel. Ministerio de Salud. División Zoonosis Rurales; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Videla, Yanina. Ministerio de Salud. División Zoonosis Rurales; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; ArgentinaFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Quintana, Silvina. Instituto de Análisis Fares Taie; ArgentinaFil: Aguirre, Pablo. Ministerio de Salud. División Zoonosis Rurales; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFaculty of Biotechnology and Food Sciences, Slovakia2019-09-05T10:58:22Z2019-09-05T10:58:22Z2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.jmbfs.org/issue/june-july-2019-vol-8-no-6/jmbfs_77_exequiel/?issue_id=5669&article_id=5http://hdl.handle.net/20.500.12123/57821338-5178https://doi.org/10.15414/jmbfs.2019.8.6.1265-1270Journal of Microbiology, Biotechnology and Food Sciences 8 (6) : 1265-1270 (Julio 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:37Zoai:localhost:20.500.12123/5782instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:37.64INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
spellingShingle Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
Scialfa, Exequiel
Leptospira
Aislamiento
Argentina
Isolation
Saprophytism
Saprofitismo
Aquaspirillum
title_short Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_full Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_fullStr Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_full_unstemmed Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_sort Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
dc.creator.none.fl_str_mv Scialfa, Exequiel
Videla, Yanina
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana Felicitas
author Scialfa, Exequiel
author_facet Scialfa, Exequiel
Videla, Yanina
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana Felicitas
author_role author
author2 Videla, Yanina
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana Felicitas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Leptospira
Aislamiento
Argentina
Isolation
Saprophytism
Saprofitismo
Aquaspirillum
topic Leptospira
Aislamiento
Argentina
Isolation
Saprophytism
Saprofitismo
Aquaspirillum
dc.description.none.fl_txt_mv Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
Instituto de Patobiología
Fil: Scialfa, Exequiel. Ministerio de Salud. División Zoonosis Rurales; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Videla, Yanina. Ministerio de Salud. División Zoonosis Rurales; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Quintana, Silvina. Instituto de Análisis Fares Taie; Argentina
Fil: Aguirre, Pablo. Ministerio de Salud. División Zoonosis Rurales; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
description Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-05T10:58:22Z
2019-09-05T10:58:22Z
2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.jmbfs.org/issue/june-july-2019-vol-8-no-6/jmbfs_77_exequiel/?issue_id=5669&article_id=5
http://hdl.handle.net/20.500.12123/5782
1338-5178
https://doi.org/10.15414/jmbfs.2019.8.6.1265-1270
url https://www.jmbfs.org/issue/june-july-2019-vol-8-no-6/jmbfs_77_exequiel/?issue_id=5669&article_id=5
http://hdl.handle.net/20.500.12123/5782
https://doi.org/10.15414/jmbfs.2019.8.6.1265-1270
identifier_str_mv 1338-5178
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculty of Biotechnology and Food Sciences, Slovakia
publisher.none.fl_str_mv Faculty of Biotechnology and Food Sciences, Slovakia
dc.source.none.fl_str_mv Journal of Microbiology, Biotechnology and Food Sciences 8 (6) : 1265-1270 (Julio 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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