Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine

Autores
Scialfa, Exequiel Alejandro; Videla, Yanina Paola; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; Brihuega, Bibiana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
Fil: Scialfa, Exequiel Alejandro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. División Zoonosis Rurales; Argentina
Fil: Videla, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. División Zoonosis Rurales; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Instituto de Análisis; Argentina
Fil: Aguirre, Pablo. División Zoonosis Rurales; Argentina
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Materia
AQUASPIRILLUM SPP.
BIOFILM
CELL AGGR
LEPTOSPIRA SPP.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184904
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184904
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of ArgentineScialfa, Exequiel AlejandroVidela, Yanina PaolaGrune Loffler, SylviaQuintana, SilvinaAguirre, PabloBrihuega, BibianaAQUASPIRILLUM SPP.BIOFILMCELL AGGRLEPTOSPIRA SPP.https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.Fil: Scialfa, Exequiel Alejandro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. División Zoonosis Rurales; ArgentinaFil: Videla, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. División Zoonosis Rurales; ArgentinaFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; ArgentinaFil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Instituto de Análisis; ArgentinaFil: Aguirre, Pablo. División Zoonosis Rurales; ArgentinaFil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaSlovak University of Agriculture2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184904Scialfa, Exequiel Alejandro; Videla, Yanina Paola; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; et al.; Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine; Slovak University of Agriculture; Journal of Microbiology, Biotechnology and Food Sciences; 8; 6; 6-2019; 1265-12701338-5178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://office2.jmbfs.org/index.php/JMBFS/article/view/8860info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2019.8.6.1265-1270info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:06:15Zoai:ri.conicet.gov.ar:11336/184904instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:06:16.21CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
title Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
spellingShingle Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
Scialfa, Exequiel Alejandro
AQUASPIRILLUM SPP.
BIOFILM
CELL AGGR
LEPTOSPIRA SPP.
title_short Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
title_full Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
title_fullStr Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
title_full_unstemmed Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
title_sort Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine
dc.creator.none.fl_str_mv Scialfa, Exequiel Alejandro
Videla, Yanina Paola
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana
author Scialfa, Exequiel Alejandro
author_facet Scialfa, Exequiel Alejandro
Videla, Yanina Paola
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana
author_role author
author2 Videla, Yanina Paola
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AQUASPIRILLUM SPP.
BIOFILM
CELL AGGR
LEPTOSPIRA SPP.
topic AQUASPIRILLUM SPP.
BIOFILM
CELL AGGR
LEPTOSPIRA SPP.
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
Fil: Scialfa, Exequiel Alejandro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. División Zoonosis Rurales; Argentina
Fil: Videla, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. División Zoonosis Rurales; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Fares Taie Instituto de Análisis; Argentina
Fil: Aguirre, Pablo. División Zoonosis Rurales; Argentina
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
description Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184904
Scialfa, Exequiel Alejandro; Videla, Yanina Paola; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; et al.; Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine; Slovak University of Agriculture; Journal of Microbiology, Biotechnology and Food Sciences; 8; 6; 6-2019; 1265-1270
1338-5178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184904
identifier_str_mv Scialfa, Exequiel Alejandro; Videla, Yanina Paola; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; et al.; Biofilm formation by Aquaspirillum Spp. and Saprophytic Leptospira Spp. isolated from environmental source Of Argentine; Slovak University of Agriculture; Journal of Microbiology, Biotechnology and Food Sciences; 8; 6; 6-2019; 1265-1270
1338-5178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://office2.jmbfs.org/index.php/JMBFS/article/view/8860
info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2019.8.6.1265-1270
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Slovak University of Agriculture
publisher.none.fl_str_mv Slovak University of Agriculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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