Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
- Autores
- Scialfa, Exequiel; Videla, Yanina; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; Brihuega, Bibiana
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
- Materia
-
Biotecnología Agrícola y Biotecnología Alimentaria
Aquaspirillum spp.,
cell aggregation
Leptospira
Biofilmes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/10057
Ver los metadatos del registro completo
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network_name_str |
CIC Digital (CICBA) |
spelling |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of ArgentineScialfa, ExequielVidela, YaninaGrune Loffler, SylviaQuintana, SilvinaAguirre, PabloBrihuega, BibianaBiotecnología Agrícola y Biotecnología AlimentariaAquaspirillum spp.,cell aggregationLeptospiraBiofilmesLeptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra)2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/10057enginfo:eu-repo/semantics/altIdentifier/issn/1338-5178Azul (Buenos Aires, Argentina)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:17Zoai:digital.cic.gba.gob.ar:11746/10057Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:18.033CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
title |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
spellingShingle |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine Scialfa, Exequiel Biotecnología Agrícola y Biotecnología Alimentaria Aquaspirillum spp., cell aggregation Leptospira Biofilmes |
title_short |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
title_full |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
title_fullStr |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
title_full_unstemmed |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
title_sort |
Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine |
dc.creator.none.fl_str_mv |
Scialfa, Exequiel Videla, Yanina Grune Loffler, Sylvia Quintana, Silvina Aguirre, Pablo Brihuega, Bibiana |
author |
Scialfa, Exequiel |
author_facet |
Scialfa, Exequiel Videla, Yanina Grune Loffler, Sylvia Quintana, Silvina Aguirre, Pablo Brihuega, Bibiana |
author_role |
author |
author2 |
Videla, Yanina Grune Loffler, Sylvia Quintana, Silvina Aguirre, Pablo Brihuega, Bibiana |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Biotecnología Agrícola y Biotecnología Alimentaria Aquaspirillum spp., cell aggregation Leptospira Biofilmes |
topic |
Biotecnología Agrícola y Biotecnología Alimentaria Aquaspirillum spp., cell aggregation Leptospira Biofilmes |
dc.description.none.fl_txt_mv |
Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires. |
description |
Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/10057 |
url |
https://digital.cic.gba.gob.ar/handle/11746/10057 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1338-5178 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Azul (Buenos Aires, Argentina) |
dc.publisher.none.fl_str_mv |
Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra) |
publisher.none.fl_str_mv |
Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra) |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
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CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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1844618615023730688 |
score |
13.070432 |