Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine

Autores
Scialfa, Exequiel; Videla, Yanina; Grune Loffler, Sylvia; Quintana, Silvina; Aguirre, Pablo; Brihuega, Bibiana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
Materia
Biotecnología Agrícola y Biotecnología Alimentaria
Aquaspirillum spp.,
cell aggregation
Leptospira
Biofilmes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/10057

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/10057
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of ArgentineScialfa, ExequielVidela, YaninaGrune Loffler, SylviaQuintana, SilvinaAguirre, PabloBrihuega, BibianaBiotecnología Agrícola y Biotecnología AlimentariaAquaspirillum spp.,cell aggregationLeptospiraBiofilmesLeptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra)2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/10057enginfo:eu-repo/semantics/altIdentifier/issn/1338-5178Azul (Buenos Aires, Argentina)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:17Zoai:digital.cic.gba.gob.ar:11746/10057Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:18.033CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
spellingShingle Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
Scialfa, Exequiel
Biotecnología Agrícola y Biotecnología Alimentaria
Aquaspirillum spp.,
cell aggregation
Leptospira
Biofilmes
title_short Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_full Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_fullStr Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_full_unstemmed Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
title_sort Biofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentine
dc.creator.none.fl_str_mv Scialfa, Exequiel
Videla, Yanina
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana
author Scialfa, Exequiel
author_facet Scialfa, Exequiel
Videla, Yanina
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana
author_role author
author2 Videla, Yanina
Grune Loffler, Sylvia
Quintana, Silvina
Aguirre, Pablo
Brihuega, Bibiana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biotecnología Agrícola y Biotecnología Alimentaria
Aquaspirillum spp.,
cell aggregation
Leptospira
Biofilmes
topic Biotecnología Agrícola y Biotecnología Alimentaria
Aquaspirillum spp.,
cell aggregation
Leptospira
Biofilmes
dc.description.none.fl_txt_mv Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
description Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4- 8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/10057
url https://digital.cic.gba.gob.ar/handle/11746/10057
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1338-5178
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Azul (Buenos Aires, Argentina)
dc.publisher.none.fl_str_mv Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra)
publisher.none.fl_str_mv Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra)
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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score 13.070432