Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus

Autores
Corley, Sean W.; Jonsson, Nicholas N.; Piper, Emily K.; Cutulle, Christian; Stear, Michael J.; Seddon, Jennifer M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the RmβAOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the RmβAOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the RmβAOR gene are likely to confer resistance to amitraz.
Instituto de Patobiología
Fil: Corley, Sean W. University of Queensland. School of Veterinary Science; Australia
Fil: Jonsson, Nicholas N. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Piper, Emily K. University of Queensland. School of Veterinary Science; Australia
Fil: Cutulle, Christian. University of Queensland. School of Veterinary Science; Australia
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Stear, Michael J. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Seddon, Jennifer M. University of Queensland. School of Veterinary Science; Australia
Fuente
Proceedings of the National Academy of Sciences 110 (42) : 16772-16777 (Octubre 2013)
Materia
Mutation
Amitraz
Pesticide Resistance
Cattle
Mutación
Resistencia a los Plaguicidas
Ganado Bovino
Rhipicephalus
Garrapatas
Ticks
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/20165
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spelling Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplusCorley, Sean W.Jonsson, Nicholas N.Piper, Emily K.Cutulle, ChristianStear, Michael J.Seddon, Jennifer M.MutationAmitrazPesticide ResistanceCattleMutaciónResistencia a los PlaguicidasGanado BovinoRhipicephalusGarrapatasTicksWe aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the RmβAOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the RmβAOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the RmβAOR gene are likely to confer resistance to amitraz.Instituto de PatobiologíaFil: Corley, Sean W. University of Queensland. School of Veterinary Science; AustraliaFil: Jonsson, Nicholas N. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino UnidoFil: Piper, Emily K. University of Queensland. School of Veterinary Science; AustraliaFil: Cutulle, Christian. University of Queensland. School of Veterinary Science; AustraliaFil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Stear, Michael J. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino UnidoFil: Seddon, Jennifer M. University of Queensland. School of Veterinary Science; AustraliaNational Academy of Sciences2024-11-07T16:06:25Z2024-11-07T16:06:25Z2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/20165https://www.pnas.org/doi/full/10.1073/pnas.13090721101091-6490https://doi.org/10.1073/pnas.1309072110Proceedings of the National Academy of Sciences 110 (42) : 16772-16777 (Octubre 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:54Zoai:localhost:20.500.12123/20165instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:54.545INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
title Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
spellingShingle Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
Corley, Sean W.
Mutation
Amitraz
Pesticide Resistance
Cattle
Mutación
Resistencia a los Plaguicidas
Ganado Bovino
Rhipicephalus
Garrapatas
Ticks
title_short Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
title_full Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
title_fullStr Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
title_full_unstemmed Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
title_sort Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
dc.creator.none.fl_str_mv Corley, Sean W.
Jonsson, Nicholas N.
Piper, Emily K.
Cutulle, Christian
Stear, Michael J.
Seddon, Jennifer M.
author Corley, Sean W.
author_facet Corley, Sean W.
Jonsson, Nicholas N.
Piper, Emily K.
Cutulle, Christian
Stear, Michael J.
Seddon, Jennifer M.
author_role author
author2 Jonsson, Nicholas N.
Piper, Emily K.
Cutulle, Christian
Stear, Michael J.
Seddon, Jennifer M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mutation
Amitraz
Pesticide Resistance
Cattle
Mutación
Resistencia a los Plaguicidas
Ganado Bovino
Rhipicephalus
Garrapatas
Ticks
topic Mutation
Amitraz
Pesticide Resistance
Cattle
Mutación
Resistencia a los Plaguicidas
Ganado Bovino
Rhipicephalus
Garrapatas
Ticks
dc.description.none.fl_txt_mv We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the RmβAOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the RmβAOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the RmβAOR gene are likely to confer resistance to amitraz.
Instituto de Patobiología
Fil: Corley, Sean W. University of Queensland. School of Veterinary Science; Australia
Fil: Jonsson, Nicholas N. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Piper, Emily K. University of Queensland. School of Veterinary Science; Australia
Fil: Cutulle, Christian. University of Queensland. School of Veterinary Science; Australia
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Stear, Michael J. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Seddon, Jennifer M. University of Queensland. School of Veterinary Science; Australia
description We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the RmβAOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the RmβAOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the RmβAOR gene are likely to confer resistance to amitraz.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2024-11-07T16:06:25Z
2024-11-07T16:06:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/20165
https://www.pnas.org/doi/full/10.1073/pnas.1309072110
1091-6490
https://doi.org/10.1073/pnas.1309072110
url http://hdl.handle.net/20.500.12123/20165
https://www.pnas.org/doi/full/10.1073/pnas.1309072110
https://doi.org/10.1073/pnas.1309072110
identifier_str_mv 1091-6490
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv Proceedings of the National Academy of Sciences 110 (42) : 16772-16777 (Octubre 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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