In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
- Autores
- Cutulle, Christian; Lovis, Léonore; D’Agostino, Beatriz Inés; Balbiani, Gabriel; Morici, Gabriel Edgardo; Citroni, Daniel; Reggi, Julio; Caracostantogolo, Jorge Luis
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.
Instituto de Patobiología
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; Suiza
Fil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Reggi, Julio. Novartis Animal Health Argentina; Argentina
Fil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina - Fuente
- Veterinary Parasitology 192 (1-3) : 296-300 (February 2013)
- Materia
-
Rhipicephalus
Control de Plagas
Resistencia a los Acaricidas
Experimentación in Vitro
Ganado Bovino
Argentina
Amitraz
Pest Control
Resistance to Acaricides
In Vitro Experimentation
Cattle
Garrapatas
Rhipicephalus microplus
Santo Tomé, Corrientes
Ticks - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/11123
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In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), ArgentinaCutulle, ChristianLovis, LéonoreD’Agostino, Beatriz InésBalbiani, GabrielMorici, Gabriel EdgardoCitroni, DanielReggi, JulioCaracostantogolo, Jorge LuisRhipicephalusControl de PlagasResistencia a los AcaricidasExperimentación in VitroGanado BovinoArgentinaAmitrazPest ControlResistance to AcaricidesIn Vitro ExperimentationCattleGarrapatasRhipicephalus microplusSanto Tomé, CorrientesTicksIn Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.Instituto de PatobiologíaFil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; SuizaFil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Reggi, Julio. Novartis Animal Health Argentina; ArgentinaFil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaElsevier2022-01-14T12:12:03Z2022-01-14T12:12:03Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11123https://www.sciencedirect.com/science/article/abs/pii/S03044017120056140304-4017https://doi.org/10.1016/j.vetpar.2012.10.014Veterinary Parasitology 192 (1-3) : 296-300 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:27Zoai:localhost:20.500.12123/11123instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:27.813INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
title |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
spellingShingle |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina Cutulle, Christian Rhipicephalus Control de Plagas Resistencia a los Acaricidas Experimentación in Vitro Ganado Bovino Argentina Amitraz Pest Control Resistance to Acaricides In Vitro Experimentation Cattle Garrapatas Rhipicephalus microplus Santo Tomé, Corrientes Ticks |
title_short |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
title_full |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
title_fullStr |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
title_full_unstemmed |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
title_sort |
In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
dc.creator.none.fl_str_mv |
Cutulle, Christian Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis |
author |
Cutulle, Christian |
author_facet |
Cutulle, Christian Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis |
author_role |
author |
author2 |
Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Rhipicephalus Control de Plagas Resistencia a los Acaricidas Experimentación in Vitro Ganado Bovino Argentina Amitraz Pest Control Resistance to Acaricides In Vitro Experimentation Cattle Garrapatas Rhipicephalus microplus Santo Tomé, Corrientes Ticks |
topic |
Rhipicephalus Control de Plagas Resistencia a los Acaricidas Experimentación in Vitro Ganado Bovino Argentina Amitraz Pest Control Resistance to Acaricides In Vitro Experimentation Cattle Garrapatas Rhipicephalus microplus Santo Tomé, Corrientes Ticks |
dc.description.none.fl_txt_mv |
In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina. Instituto de Patobiología Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; Suiza Fil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Reggi, Julio. Novartis Animal Health Argentina; Argentina Fil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina |
description |
In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02 2022-01-14T12:12:03Z 2022-01-14T12:12:03Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/11123 https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614 0304-4017 https://doi.org/10.1016/j.vetpar.2012.10.014 |
url |
http://hdl.handle.net/20.500.12123/11123 https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614 https://doi.org/10.1016/j.vetpar.2012.10.014 |
identifier_str_mv |
0304-4017 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Veterinary Parasitology 192 (1-3) : 296-300 (February 2013) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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