In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina

Autores
Cutulle, Christian; Lovis, Léonore; D’Agostino, Beatriz Inés; Balbiani, Gabriel; Morici, Gabriel Edgardo; Citroni, Daniel; Reggi, Julio; Caracostantogolo, Jorge Luis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.
Instituto de Patobiología
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; Suiza
Fil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Reggi, Julio. Novartis Animal Health Argentina; Argentina
Fil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fuente
Veterinary Parasitology 192 (1-3) : 296-300 (February 2013)
Materia
Rhipicephalus
Control de Plagas
Resistencia a los Acaricidas
Experimentación in Vitro
Ganado Bovino
Argentina
Amitraz
Pest Control
Resistance to Acaricides
In Vitro Experimentation
Cattle
Garrapatas
Rhipicephalus microplus
Santo Tomé, Corrientes
Ticks
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11123
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11123
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spelling In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), ArgentinaCutulle, ChristianLovis, LéonoreD’Agostino, Beatriz InésBalbiani, GabrielMorici, Gabriel EdgardoCitroni, DanielReggi, JulioCaracostantogolo, Jorge LuisRhipicephalusControl de PlagasResistencia a los AcaricidasExperimentación in VitroGanado BovinoArgentinaAmitrazPest ControlResistance to AcaricidesIn Vitro ExperimentationCattleGarrapatasRhipicephalus microplusSanto Tomé, CorrientesTicksIn Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.Instituto de PatobiologíaFil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; SuizaFil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Reggi, Julio. Novartis Animal Health Argentina; ArgentinaFil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaElsevier2022-01-14T12:12:03Z2022-01-14T12:12:03Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11123https://www.sciencedirect.com/science/article/abs/pii/S03044017120056140304-4017https://doi.org/10.1016/j.vetpar.2012.10.014Veterinary Parasitology 192 (1-3) : 296-300 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:50Zoai:localhost:20.500.12123/11123instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:51.332INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
title In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
spellingShingle In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
Cutulle, Christian
Rhipicephalus
Control de Plagas
Resistencia a los Acaricidas
Experimentación in Vitro
Ganado Bovino
Argentina
Amitraz
Pest Control
Resistance to Acaricides
In Vitro Experimentation
Cattle
Garrapatas
Rhipicephalus microplus
Santo Tomé, Corrientes
Ticks
title_short In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
title_full In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
title_fullStr In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
title_full_unstemmed In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
title_sort In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
dc.creator.none.fl_str_mv Cutulle, Christian
Lovis, Léonore
D’Agostino, Beatriz Inés
Balbiani, Gabriel
Morici, Gabriel Edgardo
Citroni, Daniel
Reggi, Julio
Caracostantogolo, Jorge Luis
author Cutulle, Christian
author_facet Cutulle, Christian
Lovis, Léonore
D’Agostino, Beatriz Inés
Balbiani, Gabriel
Morici, Gabriel Edgardo
Citroni, Daniel
Reggi, Julio
Caracostantogolo, Jorge Luis
author_role author
author2 Lovis, Léonore
D’Agostino, Beatriz Inés
Balbiani, Gabriel
Morici, Gabriel Edgardo
Citroni, Daniel
Reggi, Julio
Caracostantogolo, Jorge Luis
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rhipicephalus
Control de Plagas
Resistencia a los Acaricidas
Experimentación in Vitro
Ganado Bovino
Argentina
Amitraz
Pest Control
Resistance to Acaricides
In Vitro Experimentation
Cattle
Garrapatas
Rhipicephalus microplus
Santo Tomé, Corrientes
Ticks
topic Rhipicephalus
Control de Plagas
Resistencia a los Acaricidas
Experimentación in Vitro
Ganado Bovino
Argentina
Amitraz
Pest Control
Resistance to Acaricides
In Vitro Experimentation
Cattle
Garrapatas
Rhipicephalus microplus
Santo Tomé, Corrientes
Ticks
dc.description.none.fl_txt_mv In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.
Instituto de Patobiología
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; Suiza
Fil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Reggi, Julio. Novartis Animal Health Argentina; Argentina
Fil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
description In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2022-01-14T12:12:03Z
2022-01-14T12:12:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11123
https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614
0304-4017
https://doi.org/10.1016/j.vetpar.2012.10.014
url http://hdl.handle.net/20.500.12123/11123
https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614
https://doi.org/10.1016/j.vetpar.2012.10.014
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology 192 (1-3) : 296-300 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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