Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots

Autores
Vazquez, Maria Magdalena; Casalongue, Claudia; Paris, Ramiro
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant roots respond positively to gravity force and orientate it growth providing anchorage to the soil and gathering water and nutrient sources. The gravitropic response is a complex process wherein nitric oxide (NO) participates as a key signaling molecule. Here, we used genetically impaired genotypes todemonstrate the role of the nitrate reductase (NR) enzyme as a possible source of endogenous NO during gravitropic response in Arabidopsis thaliana (A. thaliana) roots. A. thaliana has two NR genes, NIA1and NIA2. The single mutants nia1 and nia2, and the double mutant nia1/nia2 showed perturbed gravitropism. Complementation with the exogenous NO donor, S-nitroso-L-cysteine, partially rescued the wild-type phenotype in nia2 and nia1/nia2 but not in the nia1 mutant. Our findings showed that each NR gene differentially contributes to reaching the optimum level of NO during the gravitropic response, suggesting that NIA1 and NIA2 isoforms are not equivalent and have potential regulatory feedback to each other during the gravitropic response in A. thaliana roots.
Fil: Vazquez, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Paris, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
ARABIDOPSIS THALIANA
GRAVITROPISM
NITRATE REDUCTASE
NITRIC OXIDE
ROOT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103541
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana rootsVazquez, Maria MagdalenaCasalongue, ClaudiaParis, RamiroARABIDOPSIS THALIANAGRAVITROPISMNITRATE REDUCTASENITRIC OXIDEROOThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant roots respond positively to gravity force and orientate it growth providing anchorage to the soil and gathering water and nutrient sources. The gravitropic response is a complex process wherein nitric oxide (NO) participates as a key signaling molecule. Here, we used genetically impaired genotypes todemonstrate the role of the nitrate reductase (NR) enzyme as a possible source of endogenous NO during gravitropic response in Arabidopsis thaliana (A. thaliana) roots. A. thaliana has two NR genes, NIA1and NIA2. The single mutants nia1 and nia2, and the double mutant nia1/nia2 showed perturbed gravitropism. Complementation with the exogenous NO donor, S-nitroso-L-cysteine, partially rescued the wild-type phenotype in nia2 and nia1/nia2 but not in the nia1 mutant. Our findings showed that each NR gene differentially contributes to reaching the optimum level of NO during the gravitropic response, suggesting that NIA1 and NIA2 isoforms are not equivalent and have potential regulatory feedback to each other during the gravitropic response in A. thaliana roots.Fil: Vazquez, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Paris, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaTaylor & Francis Group2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103541Vazquez, Maria Magdalena; Casalongue, Claudia; Paris, Ramiro; Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots; Taylor & Francis Group; Plant Signaling and Behaivor; 2-2019; 1-61559-2324CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/15592324.2019.1578631info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15592324.2019.1578631info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:45Zoai:ri.conicet.gov.ar:11336/103541instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:45.404CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
title Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
spellingShingle Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
Vazquez, Maria Magdalena
ARABIDOPSIS THALIANA
GRAVITROPISM
NITRATE REDUCTASE
NITRIC OXIDE
ROOT
title_short Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
title_full Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
title_fullStr Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
title_full_unstemmed Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
title_sort Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots
dc.creator.none.fl_str_mv Vazquez, Maria Magdalena
Casalongue, Claudia
Paris, Ramiro
author Vazquez, Maria Magdalena
author_facet Vazquez, Maria Magdalena
Casalongue, Claudia
Paris, Ramiro
author_role author
author2 Casalongue, Claudia
Paris, Ramiro
author2_role author
author
dc.subject.none.fl_str_mv ARABIDOPSIS THALIANA
GRAVITROPISM
NITRATE REDUCTASE
NITRIC OXIDE
ROOT
topic ARABIDOPSIS THALIANA
GRAVITROPISM
NITRATE REDUCTASE
NITRIC OXIDE
ROOT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant roots respond positively to gravity force and orientate it growth providing anchorage to the soil and gathering water and nutrient sources. The gravitropic response is a complex process wherein nitric oxide (NO) participates as a key signaling molecule. Here, we used genetically impaired genotypes todemonstrate the role of the nitrate reductase (NR) enzyme as a possible source of endogenous NO during gravitropic response in Arabidopsis thaliana (A. thaliana) roots. A. thaliana has two NR genes, NIA1and NIA2. The single mutants nia1 and nia2, and the double mutant nia1/nia2 showed perturbed gravitropism. Complementation with the exogenous NO donor, S-nitroso-L-cysteine, partially rescued the wild-type phenotype in nia2 and nia1/nia2 but not in the nia1 mutant. Our findings showed that each NR gene differentially contributes to reaching the optimum level of NO during the gravitropic response, suggesting that NIA1 and NIA2 isoforms are not equivalent and have potential regulatory feedback to each other during the gravitropic response in A. thaliana roots.
Fil: Vazquez, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Paris, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Plant roots respond positively to gravity force and orientate it growth providing anchorage to the soil and gathering water and nutrient sources. The gravitropic response is a complex process wherein nitric oxide (NO) participates as a key signaling molecule. Here, we used genetically impaired genotypes todemonstrate the role of the nitrate reductase (NR) enzyme as a possible source of endogenous NO during gravitropic response in Arabidopsis thaliana (A. thaliana) roots. A. thaliana has two NR genes, NIA1and NIA2. The single mutants nia1 and nia2, and the double mutant nia1/nia2 showed perturbed gravitropism. Complementation with the exogenous NO donor, S-nitroso-L-cysteine, partially rescued the wild-type phenotype in nia2 and nia1/nia2 but not in the nia1 mutant. Our findings showed that each NR gene differentially contributes to reaching the optimum level of NO during the gravitropic response, suggesting that NIA1 and NIA2 isoforms are not equivalent and have potential regulatory feedback to each other during the gravitropic response in A. thaliana roots.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103541
Vazquez, Maria Magdalena; Casalongue, Claudia; Paris, Ramiro; Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots; Taylor & Francis Group; Plant Signaling and Behaivor; 2-2019; 1-6
1559-2324
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103541
identifier_str_mv Vazquez, Maria Magdalena; Casalongue, Claudia; Paris, Ramiro; Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots; Taylor & Francis Group; Plant Signaling and Behaivor; 2-2019; 1-6
1559-2324
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/15592324.2019.1578631
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15592324.2019.1578631
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Group
publisher.none.fl_str_mv Taylor & Francis Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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