Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil

• Autores: Ortiz, Alex Henrique Tiene; Rocha, Vanesca Priscila Camargo; Moiana, Leonel Domingos; Vidigal, Maria Celeste Gonçalves; Galvan, Marta Zulema; Vidigal Filho, Pedro Soares
• Año de publicación: 2016
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plant Molecular Biology Reporter 34 (6) : 1153–1166 (December 2016)
• Materia: Mandioca; Variedades; Genética; Variación Genética; Marcadores Genéticos; Polimorfismo; Cassava; Varieties; Genetics; Genetic Variation; Genetic Markers; Polymorphism; Marcadores Moleculares; Mandioca Dulce; Brasil
• Institución: Instituto Nacional de Tecnología Agropecuaria

The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The F is (0.170) and F it (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá.
EEA Salta
Fil: Ortiz, Alex Henrique Tiene. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil
Fil: Rocha, Vanesca Priscila Camargo. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil
Fil: Moiana, Leonel Domingos. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil. Instituto de Investigação Agrária; Mozambique
Fil: Vidigal, Maria Celeste Gonçalves. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil
Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina.
Fil: Vidigal Filho, Pedro Soares. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil