Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil

Autores
Ortiz, Alex Henrique Tiene; Rocha, Vanesca Priscila Camargo; Moiana, Leonel Domingos; Gonçalves Vidigal, Maria Celeste; Galván, Marta Zulema; Vidigal Filho, Pedro Soares
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The Fis (0.170) and Fit (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá.
Fil: Ortiz, Alex Henrique Tiene. Universidade Estadual de Maringá; Brasil
Fil: Rocha, Vanesca Priscila Camargo. Universidade Estadual de Maringá; Brasil
Fil: Moiana, Leonel Domingos. Universidade Estadual de Maringá; Brasil. Instituto de Investigação Agrária; Mozambique
Fil: Gonçalves Vidigal, Maria Celeste. Universidade Estadual de Maringá; Brasil
Fil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina
Fil: Vidigal Filho, Pedro Soares. Universidade Estadual de Maringá; Brasil
Materia
Genetic Diversity
Manihot Esculenta
Polymorphism
Ssr Markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59172
Acceder
id CONICETDig_840bb214d3560785e3b23d0ab54d7cab
oai_identifier_str oai:ri.conicet.gov.ar:11336/59172
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, BrazilOrtiz, Alex Henrique TieneRocha, Vanesca Priscila CamargoMoiana, Leonel DomingosGonçalves Vidigal, Maria CelesteGalván, Marta ZulemaVidigal Filho, Pedro SoaresGenetic DiversityManihot EsculentaPolymorphismSsr Markershttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The Fis (0.170) and Fit (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá.Fil: Ortiz, Alex Henrique Tiene. Universidade Estadual de Maringá; BrasilFil: Rocha, Vanesca Priscila Camargo. Universidade Estadual de Maringá; BrasilFil: Moiana, Leonel Domingos. Universidade Estadual de Maringá; Brasil. Instituto de Investigação Agrária; MozambiqueFil: Gonçalves Vidigal, Maria Celeste. Universidade Estadual de Maringá; BrasilFil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; ArgentinaFil: Vidigal Filho, Pedro Soares. Universidade Estadual de Maringá; BrasilSpringer2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59172Ortiz, Alex Henrique Tiene; Rocha, Vanesca Priscila Camargo; Moiana, Leonel Domingos; Gonçalves Vidigal, Maria Celeste; Galván, Marta Zulema; et al.; Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil; Springer; Plant Molecular Biology Reporter; 34; 6; 12-2016; 1153-11660735-96401572-9818CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11105-016-0995-xinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11105-016-0995-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:41Zoai:ri.conicet.gov.ar:11336/59172instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:41.612CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
title Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
spellingShingle Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
Ortiz, Alex Henrique Tiene
Genetic Diversity
Manihot Esculenta
Polymorphism
Ssr Markers
title_short Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
title_full Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
title_fullStr Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
title_full_unstemmed Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
title_sort Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
dc.creator.none.fl_str_mv Ortiz, Alex Henrique Tiene
Rocha, Vanesca Priscila Camargo
Moiana, Leonel Domingos
Gonçalves Vidigal, Maria Celeste
Galván, Marta Zulema
Vidigal Filho, Pedro Soares
author Ortiz, Alex Henrique Tiene
author_facet Ortiz, Alex Henrique Tiene
Rocha, Vanesca Priscila Camargo
Moiana, Leonel Domingos
Gonçalves Vidigal, Maria Celeste
Galván, Marta Zulema
Vidigal Filho, Pedro Soares
author_role author
author2 Rocha, Vanesca Priscila Camargo
Moiana, Leonel Domingos
Gonçalves Vidigal, Maria Celeste
Galván, Marta Zulema
Vidigal Filho, Pedro Soares
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Genetic Diversity
Manihot Esculenta
Polymorphism
Ssr Markers
topic Genetic Diversity
Manihot Esculenta
Polymorphism
Ssr Markers
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The Fis (0.170) and Fit (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá.
Fil: Ortiz, Alex Henrique Tiene. Universidade Estadual de Maringá; Brasil
Fil: Rocha, Vanesca Priscila Camargo. Universidade Estadual de Maringá; Brasil
Fil: Moiana, Leonel Domingos. Universidade Estadual de Maringá; Brasil. Instituto de Investigação Agrária; Mozambique
Fil: Gonçalves Vidigal, Maria Celeste. Universidade Estadual de Maringá; Brasil
Fil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina
Fil: Vidigal Filho, Pedro Soares. Universidade Estadual de Maringá; Brasil
description The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The Fis (0.170) and Fit (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59172
Ortiz, Alex Henrique Tiene; Rocha, Vanesca Priscila Camargo; Moiana, Leonel Domingos; Gonçalves Vidigal, Maria Celeste; Galván, Marta Zulema; et al.; Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil; Springer; Plant Molecular Biology Reporter; 34; 6; 12-2016; 1153-1166
0735-9640
1572-9818
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59172
identifier_str_mv Ortiz, Alex Henrique Tiene; Rocha, Vanesca Priscila Camargo; Moiana, Leonel Domingos; Gonçalves Vidigal, Maria Celeste; Galván, Marta Zulema; et al.; Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil; Springer; Plant Molecular Biology Reporter; 34; 6; 12-2016; 1153-1166
0735-9640
1572-9818
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11105-016-0995-x
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11105-016-0995-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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