Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome

Autores
Berruezo, Silvia; Ferreyra, Susana; Fontana, Ariel; Paola, Matilde de; Perez, María Belén; Scapini, Celina; Cremer, Cecilia; Castro, Claudia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditionally used as hyperglycemic and anti-inflammatory, could have beneficial effects on the treatment of MetS. Purpose: The present study was aimed to characterize the composition of M. spinosum roots decoction, and to evaluate antidiabetic, antilipemic and antioxidant effects in an animal model of MetS. Study design and methods: M. spinosum roots extract was characterized using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method. Total Phenolic Content (TPC) was spectrophotometrically measured and the antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC) assay. To generate the MetS model, adult male C57BL/6 mice were fed with a 20% w/v fructose (Fru) in drinking water combined with 30% w/w high fat diet (HFD) over a period of 12 weeks. M. spinosum aqueous extracts (3 or 6 g/kg/day) were administered in drinking water together with Fru-HFD. At the end of the exposure period, mice were weighed and glucose tolerance test was determined. After sacrifice, adipose tissues were isolated and blood samples were collected to evaluate lipid profile, lipid peroxidation level (LPO) and ferric reducing antioxidant power (FRAP). Results: The characterization of M. spinosum revealed the presence of caffeic acid, trans-resveratrol, kaempferol-3-glucoside, (-)-epicatechin, (-)-gallocatechin gallate, (-)-epigallocatechin and (+)-catechin. A high antioxidant capacity of the extract was revealed by ORAC test. Mice fed a Fru-HF diet and treated with M. spinosum, reduced diet-induced weight gain and significantly decreased mesenteric fat compared with Fru-HFD group (p<0.01). Post prandial glycaemia significantly diminished when mice were treated with M. spinosum, and also total cholesterol, LDL-cholesterol and triglycerides (TG) were reduced. Regarding the antioxidant effect of the consumption of M. spinosum, we found a decrease in plasmatic LPO, and a significant increase in plasmatic FRAP. Conclusion: To our knowledge, the phenolic composition and the antioxidant capacity of M. spinosum roots decoction is reported for the first time. In addition, we provide evidence that M. spinosum root extract is a promising source of antioxidants able to alleviates Fru-HFD-induced metabolic alterations and slow the progression of MetS.
EEA La Consulta
Fil: Berruezo, Silvia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina
Fil: Ferreyra, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ferreyra, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Paola, Matilde de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Paola, Matilde de. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; Argentina
Fil: Pérez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Scapini, Celina. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina
Fil: Cremer, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina
Fil: Castro, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Castro, Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; Argentina
Fuente
Phytomedicine Plus 2 (1) : 100169 (February 2022)
Materia
Extractos Vegetales
Control de Enfermedades
Trastornos Metabólicos
Obesidad
Propiedades Antioxidantes
Plant Extracts
Disease Control
Metabolic Disorders
Obesity
Antioxidant Properties
Síndrome Metabólico
Mulinum spinosum
Metabolic Syndrome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10852
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10852
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndromeBerruezo, SilviaFerreyra, SusanaFontana, ArielPaola, Matilde dePerez, María BelénScapini, CelinaCremer, CeciliaCastro, ClaudiaExtractos VegetalesControl de EnfermedadesTrastornos MetabólicosObesidadPropiedades AntioxidantesPlant ExtractsDisease ControlMetabolic DisordersObesityAntioxidant PropertiesSíndrome MetabólicoMulinum spinosumMetabolic SyndromeBackground: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditionally used as hyperglycemic and anti-inflammatory, could have beneficial effects on the treatment of MetS. Purpose: The present study was aimed to characterize the composition of M. spinosum roots decoction, and to evaluate antidiabetic, antilipemic and antioxidant effects in an animal model of MetS. Study design and methods: M. spinosum roots extract was characterized using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method. Total Phenolic Content (TPC) was spectrophotometrically measured and the antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC) assay. To generate the MetS model, adult male C57BL/6 mice were fed with a 20% w/v fructose (Fru) in drinking water combined with 30% w/w high fat diet (HFD) over a period of 12 weeks. M. spinosum aqueous extracts (3 or 6 g/kg/day) were administered in drinking water together with Fru-HFD. At the end of the exposure period, mice were weighed and glucose tolerance test was determined. After sacrifice, adipose tissues were isolated and blood samples were collected to evaluate lipid profile, lipid peroxidation level (LPO) and ferric reducing antioxidant power (FRAP). Results: The characterization of M. spinosum revealed the presence of caffeic acid, trans-resveratrol, kaempferol-3-glucoside, (-)-epicatechin, (-)-gallocatechin gallate, (-)-epigallocatechin and (+)-catechin. A high antioxidant capacity of the extract was revealed by ORAC test. Mice fed a Fru-HF diet and treated with M. spinosum, reduced diet-induced weight gain and significantly decreased mesenteric fat compared with Fru-HFD group (p<0.01). Post prandial glycaemia significantly diminished when mice were treated with M. spinosum, and also total cholesterol, LDL-cholesterol and triglycerides (TG) were reduced. Regarding the antioxidant effect of the consumption of M. spinosum, we found a decrease in plasmatic LPO, and a significant increase in plasmatic FRAP. Conclusion: To our knowledge, the phenolic composition and the antioxidant capacity of M. spinosum roots decoction is reported for the first time. In addition, we provide evidence that M. spinosum root extract is a promising source of antioxidants able to alleviates Fru-HFD-induced metabolic alterations and slow the progression of MetS.EEA La ConsultaFil: Berruezo, Silvia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; ArgentinaFil: Ferreyra, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ferreyra, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fontana, Ariel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Paola, Matilde de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Paola, Matilde de. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; ArgentinaFil: Pérez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Scapini, Celina. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; ArgentinaFil: Cremer, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; ArgentinaFil: Castro, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Castro, Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; ArgentinaElsevier2021-12-06T11:52:22Z2021-12-06T11:52:22Z2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10852https://www.sciencedirect.com/science/article/pii/S26670313210015122667-0313https://doi.org/10.1016/j.phyplu.2021.100169Phytomedicine Plus 2 (1) : 100169 (February 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:12Zoai:localhost:20.500.12123/10852instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:12.76INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
title Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
spellingShingle Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
Berruezo, Silvia
Extractos Vegetales
Control de Enfermedades
Trastornos Metabólicos
Obesidad
Propiedades Antioxidantes
Plant Extracts
Disease Control
Metabolic Disorders
Obesity
Antioxidant Properties
Síndrome Metabólico
Mulinum spinosum
Metabolic Syndrome
title_short Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
title_full Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
title_fullStr Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
title_full_unstemmed Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
title_sort Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
dc.creator.none.fl_str_mv Berruezo, Silvia
Ferreyra, Susana
Fontana, Ariel
Paola, Matilde de
Perez, María Belén
Scapini, Celina
Cremer, Cecilia
Castro, Claudia
author Berruezo, Silvia
author_facet Berruezo, Silvia
Ferreyra, Susana
Fontana, Ariel
Paola, Matilde de
Perez, María Belén
Scapini, Celina
Cremer, Cecilia
Castro, Claudia
author_role author
author2 Ferreyra, Susana
Fontana, Ariel
Paola, Matilde de
Perez, María Belén
Scapini, Celina
Cremer, Cecilia
Castro, Claudia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Extractos Vegetales
Control de Enfermedades
Trastornos Metabólicos
Obesidad
Propiedades Antioxidantes
Plant Extracts
Disease Control
Metabolic Disorders
Obesity
Antioxidant Properties
Síndrome Metabólico
Mulinum spinosum
Metabolic Syndrome
topic Extractos Vegetales
Control de Enfermedades
Trastornos Metabólicos
Obesidad
Propiedades Antioxidantes
Plant Extracts
Disease Control
Metabolic Disorders
Obesity
Antioxidant Properties
Síndrome Metabólico
Mulinum spinosum
Metabolic Syndrome
dc.description.none.fl_txt_mv Background: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditionally used as hyperglycemic and anti-inflammatory, could have beneficial effects on the treatment of MetS. Purpose: The present study was aimed to characterize the composition of M. spinosum roots decoction, and to evaluate antidiabetic, antilipemic and antioxidant effects in an animal model of MetS. Study design and methods: M. spinosum roots extract was characterized using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method. Total Phenolic Content (TPC) was spectrophotometrically measured and the antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC) assay. To generate the MetS model, adult male C57BL/6 mice were fed with a 20% w/v fructose (Fru) in drinking water combined with 30% w/w high fat diet (HFD) over a period of 12 weeks. M. spinosum aqueous extracts (3 or 6 g/kg/day) were administered in drinking water together with Fru-HFD. At the end of the exposure period, mice were weighed and glucose tolerance test was determined. After sacrifice, adipose tissues were isolated and blood samples were collected to evaluate lipid profile, lipid peroxidation level (LPO) and ferric reducing antioxidant power (FRAP). Results: The characterization of M. spinosum revealed the presence of caffeic acid, trans-resveratrol, kaempferol-3-glucoside, (-)-epicatechin, (-)-gallocatechin gallate, (-)-epigallocatechin and (+)-catechin. A high antioxidant capacity of the extract was revealed by ORAC test. Mice fed a Fru-HF diet and treated with M. spinosum, reduced diet-induced weight gain and significantly decreased mesenteric fat compared with Fru-HFD group (p<0.01). Post prandial glycaemia significantly diminished when mice were treated with M. spinosum, and also total cholesterol, LDL-cholesterol and triglycerides (TG) were reduced. Regarding the antioxidant effect of the consumption of M. spinosum, we found a decrease in plasmatic LPO, and a significant increase in plasmatic FRAP. Conclusion: To our knowledge, the phenolic composition and the antioxidant capacity of M. spinosum roots decoction is reported for the first time. In addition, we provide evidence that M. spinosum root extract is a promising source of antioxidants able to alleviates Fru-HFD-induced metabolic alterations and slow the progression of MetS.
EEA La Consulta
Fil: Berruezo, Silvia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina
Fil: Ferreyra, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ferreyra, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Paola, Matilde de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Paola, Matilde de. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; Argentina
Fil: Pérez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Scapini, Celina. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina
Fil: Cremer, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina
Fil: Castro, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Castro, Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; Argentina
description Background: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditionally used as hyperglycemic and anti-inflammatory, could have beneficial effects on the treatment of MetS. Purpose: The present study was aimed to characterize the composition of M. spinosum roots decoction, and to evaluate antidiabetic, antilipemic and antioxidant effects in an animal model of MetS. Study design and methods: M. spinosum roots extract was characterized using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method. Total Phenolic Content (TPC) was spectrophotometrically measured and the antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC) assay. To generate the MetS model, adult male C57BL/6 mice were fed with a 20% w/v fructose (Fru) in drinking water combined with 30% w/w high fat diet (HFD) over a period of 12 weeks. M. spinosum aqueous extracts (3 or 6 g/kg/day) were administered in drinking water together with Fru-HFD. At the end of the exposure period, mice were weighed and glucose tolerance test was determined. After sacrifice, adipose tissues were isolated and blood samples were collected to evaluate lipid profile, lipid peroxidation level (LPO) and ferric reducing antioxidant power (FRAP). Results: The characterization of M. spinosum revealed the presence of caffeic acid, trans-resveratrol, kaempferol-3-glucoside, (-)-epicatechin, (-)-gallocatechin gallate, (-)-epigallocatechin and (+)-catechin. A high antioxidant capacity of the extract was revealed by ORAC test. Mice fed a Fru-HF diet and treated with M. spinosum, reduced diet-induced weight gain and significantly decreased mesenteric fat compared with Fru-HFD group (p<0.01). Post prandial glycaemia significantly diminished when mice were treated with M. spinosum, and also total cholesterol, LDL-cholesterol and triglycerides (TG) were reduced. Regarding the antioxidant effect of the consumption of M. spinosum, we found a decrease in plasmatic LPO, and a significant increase in plasmatic FRAP. Conclusion: To our knowledge, the phenolic composition and the antioxidant capacity of M. spinosum roots decoction is reported for the first time. In addition, we provide evidence that M. spinosum root extract is a promising source of antioxidants able to alleviates Fru-HFD-induced metabolic alterations and slow the progression of MetS.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-06T11:52:22Z
2021-12-06T11:52:22Z
2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10852
https://www.sciencedirect.com/science/article/pii/S2667031321001512
2667-0313
https://doi.org/10.1016/j.phyplu.2021.100169
url http://hdl.handle.net/20.500.12123/10852
https://www.sciencedirect.com/science/article/pii/S2667031321001512
https://doi.org/10.1016/j.phyplu.2021.100169
identifier_str_mv 2667-0313
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Phytomedicine Plus 2 (1) : 100169 (February 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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