Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection

Autores
Gismondi, Maria Ines; Ortiz, Xoana P.; Curra, Anabella Paola; Asurmendi, Sebastian; Taboga, Oscar Alberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA.
Instituto de Biotecnología
Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ortiz, Xoana P. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Journal of virological methods 199 : 1-10. (April 2014)
Materia
ARN
Genética
Virus de las Plantas
Viricidas
RNA
Genetics
Plant Viruses
Antiviral Agents
MicroRNA
Ácido Ribonucléico
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2277
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2277
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network_name_str INTA Digital (INTA)
spelling Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selectionGismondi, Maria InesOrtiz, Xoana P.Curra, Anabella PaolaAsurmendi, SebastianTaboga, Oscar AlbertoARNGenéticaVirus de las PlantasViricidasRNAGeneticsPlant VirusesAntiviral AgentsMicroRNAÁcido RibonucléicoRNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA.Instituto de BiotecnologíaFil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Ortiz, Xoana P. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2018-04-19T13:27:48Z2018-04-19T13:27:48Z2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0166093413005181#!http://hdl.handle.net/20.500.12123/22770166-0934https://doi.org/10.1016/j.jviromet.2013.12.016Journal of virological methods 199 : 1-10. (April 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:12Zoai:localhost:20.500.12123/2277instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:13.311INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
spellingShingle Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
Gismondi, Maria Ines
ARN
Genética
Virus de las Plantas
Viricidas
RNA
Genetics
Plant Viruses
Antiviral Agents
MicroRNA
Ácido Ribonucléico
title_short Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_full Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_fullStr Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_full_unstemmed Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_sort Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
dc.creator.none.fl_str_mv Gismondi, Maria Ines
Ortiz, Xoana P.
Curra, Anabella Paola
Asurmendi, Sebastian
Taboga, Oscar Alberto
author Gismondi, Maria Ines
author_facet Gismondi, Maria Ines
Ortiz, Xoana P.
Curra, Anabella Paola
Asurmendi, Sebastian
Taboga, Oscar Alberto
author_role author
author2 Ortiz, Xoana P.
Curra, Anabella Paola
Asurmendi, Sebastian
Taboga, Oscar Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARN
Genética
Virus de las Plantas
Viricidas
RNA
Genetics
Plant Viruses
Antiviral Agents
MicroRNA
Ácido Ribonucléico
topic ARN
Genética
Virus de las Plantas
Viricidas
RNA
Genetics
Plant Viruses
Antiviral Agents
MicroRNA
Ácido Ribonucléico
dc.description.none.fl_txt_mv RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA.
Instituto de Biotecnología
Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ortiz, Xoana P. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
2018-04-19T13:27:48Z
2018-04-19T13:27:48Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0166093413005181#!
http://hdl.handle.net/20.500.12123/2277
0166-0934
https://doi.org/10.1016/j.jviromet.2013.12.016
url https://www.sciencedirect.com/science/article/pii/S0166093413005181#!
http://hdl.handle.net/20.500.12123/2277
https://doi.org/10.1016/j.jviromet.2013.12.016
identifier_str_mv 0166-0934
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of virological methods 199 : 1-10. (April 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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