Plant microRNAs: Recent Advances and Future Challenges

Autores
Debat, Humberto Julio; Ducasse, Daniel Adrian
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MicroRNAs (miRNAs) are small ∼20–24 nt species of non-coding RNAs that modulate plant gene expression by means of gene silencing through sequence-specific inhibition of target mRNAs. MiRNAs derive from pol-II transcription of non-coding genes that are precisely processed in nuclear Dicing bodies by a microprocessor complex (dicer-like1–serrate–hyponastic leaves 1: DCL1-SE-HYL1), which recognizes stem-loop secondary-structure features of primary precursor miRNA transcripts (pri-miRNA). The proper processing of the pri-miRNAs results in a double-stranded small RNA that will eventually exit the nucleus and be loaded predominantly onto the effector complex Argonaute1 (Ago1). The single-stranded mature miRNA will guide AGO1, leading to cleavage or translational arrest of complementary mRNAs. MiRNA steady-state levels and activity are regulated not only by transcription rate of precursor transcripts, but also by direct degradation mediated by small RNA degrading nuclease1 (SDN1). miRNAs are retailored by 3′ editing through 2-O-methylation, uridylation and adenlylation, involving Hua enhancer1 (HEN1), HEN1 suppressor1 (HESO1) and probably the exosome—a phenomenon that has been elucidated only scarcely to date in Arabidopsis. MiRNA activity is involved not only in plant development, but also in signaling, abiotic stresses such as drought, heat and metal toxicity, pathogen interaction and symbiotic relationship regulation, among others. The engineering of miRNAs is paving the way to next-generation plant biotechnology by means of over-expression of natural miRNAs, generation of artificial microRNAs and inhibition of miRNA activity by target mimicry. This review highlights the importance of miRNAs in plant sciences by describing the latest updates in this research field.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Plant Molecular Biology Reporter 32 (6) : 1257–1269 (December 2014)
Materia
ARN
Genética
Expresión Génica
Biotecnología
RNA
Genetics
Gene Expression
Biotechnology
Ácido Ribonucléico
MicroRNA
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2239
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2239
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network_name_str INTA Digital (INTA)
spelling Plant microRNAs: Recent Advances and Future ChallengesDebat, Humberto JulioDucasse, Daniel AdrianARNGenéticaExpresión GénicaBiotecnologíaRNAGeneticsGene ExpressionBiotechnologyÁcido RibonucléicoMicroRNAMicroRNAs (miRNAs) are small ∼20–24 nt species of non-coding RNAs that modulate plant gene expression by means of gene silencing through sequence-specific inhibition of target mRNAs. MiRNAs derive from pol-II transcription of non-coding genes that are precisely processed in nuclear Dicing bodies by a microprocessor complex (dicer-like1–serrate–hyponastic leaves 1: DCL1-SE-HYL1), which recognizes stem-loop secondary-structure features of primary precursor miRNA transcripts (pri-miRNA). The proper processing of the pri-miRNAs results in a double-stranded small RNA that will eventually exit the nucleus and be loaded predominantly onto the effector complex Argonaute1 (Ago1). The single-stranded mature miRNA will guide AGO1, leading to cleavage or translational arrest of complementary mRNAs. MiRNA steady-state levels and activity are regulated not only by transcription rate of precursor transcripts, but also by direct degradation mediated by small RNA degrading nuclease1 (SDN1). miRNAs are retailored by 3′ editing through 2-O-methylation, uridylation and adenlylation, involving Hua enhancer1 (HEN1), HEN1 suppressor1 (HESO1) and probably the exosome—a phenomenon that has been elucidated only scarcely to date in Arabidopsis. MiRNA activity is involved not only in plant development, but also in signaling, abiotic stresses such as drought, heat and metal toxicity, pathogen interaction and symbiotic relationship regulation, among others. The engineering of miRNAs is paving the way to next-generation plant biotechnology by means of over-expression of natural miRNAs, generation of artificial microRNAs and inhibition of miRNA activity by target mimicry. This review highlights the importance of miRNAs in plant sciences by describing the latest updates in this research field.Instituto de Patología VegetalFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina2018-04-13T12:06:18Z2018-04-13T12:06:18Z2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s11105-014-0727-zhttp://hdl.handle.net/20.500.12123/22390735-9640 (Print)1572-9818 (Online)https://doi.org/10.1007/s11105-014-0727-zPlant Molecular Biology Reporter 32 (6) : 1257–1269 (December 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:30Zoai:localhost:20.500.12123/2239instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:30.77INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Plant microRNAs: Recent Advances and Future Challenges
title Plant microRNAs: Recent Advances and Future Challenges
spellingShingle Plant microRNAs: Recent Advances and Future Challenges
Debat, Humberto Julio
ARN
Genética
Expresión Génica
Biotecnología
RNA
Genetics
Gene Expression
Biotechnology
Ácido Ribonucléico
MicroRNA
title_short Plant microRNAs: Recent Advances and Future Challenges
title_full Plant microRNAs: Recent Advances and Future Challenges
title_fullStr Plant microRNAs: Recent Advances and Future Challenges
title_full_unstemmed Plant microRNAs: Recent Advances and Future Challenges
title_sort Plant microRNAs: Recent Advances and Future Challenges
dc.creator.none.fl_str_mv Debat, Humberto Julio
Ducasse, Daniel Adrian
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Ducasse, Daniel Adrian
author_role author
author2 Ducasse, Daniel Adrian
author2_role author
dc.subject.none.fl_str_mv ARN
Genética
Expresión Génica
Biotecnología
RNA
Genetics
Gene Expression
Biotechnology
Ácido Ribonucléico
MicroRNA
topic ARN
Genética
Expresión Génica
Biotecnología
RNA
Genetics
Gene Expression
Biotechnology
Ácido Ribonucléico
MicroRNA
dc.description.none.fl_txt_mv MicroRNAs (miRNAs) are small ∼20–24 nt species of non-coding RNAs that modulate plant gene expression by means of gene silencing through sequence-specific inhibition of target mRNAs. MiRNAs derive from pol-II transcription of non-coding genes that are precisely processed in nuclear Dicing bodies by a microprocessor complex (dicer-like1–serrate–hyponastic leaves 1: DCL1-SE-HYL1), which recognizes stem-loop secondary-structure features of primary precursor miRNA transcripts (pri-miRNA). The proper processing of the pri-miRNAs results in a double-stranded small RNA that will eventually exit the nucleus and be loaded predominantly onto the effector complex Argonaute1 (Ago1). The single-stranded mature miRNA will guide AGO1, leading to cleavage or translational arrest of complementary mRNAs. MiRNA steady-state levels and activity are regulated not only by transcription rate of precursor transcripts, but also by direct degradation mediated by small RNA degrading nuclease1 (SDN1). miRNAs are retailored by 3′ editing through 2-O-methylation, uridylation and adenlylation, involving Hua enhancer1 (HEN1), HEN1 suppressor1 (HESO1) and probably the exosome—a phenomenon that has been elucidated only scarcely to date in Arabidopsis. MiRNA activity is involved not only in plant development, but also in signaling, abiotic stresses such as drought, heat and metal toxicity, pathogen interaction and symbiotic relationship regulation, among others. The engineering of miRNAs is paving the way to next-generation plant biotechnology by means of over-expression of natural miRNAs, generation of artificial microRNAs and inhibition of miRNA activity by target mimicry. This review highlights the importance of miRNAs in plant sciences by describing the latest updates in this research field.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description MicroRNAs (miRNAs) are small ∼20–24 nt species of non-coding RNAs that modulate plant gene expression by means of gene silencing through sequence-specific inhibition of target mRNAs. MiRNAs derive from pol-II transcription of non-coding genes that are precisely processed in nuclear Dicing bodies by a microprocessor complex (dicer-like1–serrate–hyponastic leaves 1: DCL1-SE-HYL1), which recognizes stem-loop secondary-structure features of primary precursor miRNA transcripts (pri-miRNA). The proper processing of the pri-miRNAs results in a double-stranded small RNA that will eventually exit the nucleus and be loaded predominantly onto the effector complex Argonaute1 (Ago1). The single-stranded mature miRNA will guide AGO1, leading to cleavage or translational arrest of complementary mRNAs. MiRNA steady-state levels and activity are regulated not only by transcription rate of precursor transcripts, but also by direct degradation mediated by small RNA degrading nuclease1 (SDN1). miRNAs are retailored by 3′ editing through 2-O-methylation, uridylation and adenlylation, involving Hua enhancer1 (HEN1), HEN1 suppressor1 (HESO1) and probably the exosome—a phenomenon that has been elucidated only scarcely to date in Arabidopsis. MiRNA activity is involved not only in plant development, but also in signaling, abiotic stresses such as drought, heat and metal toxicity, pathogen interaction and symbiotic relationship regulation, among others. The engineering of miRNAs is paving the way to next-generation plant biotechnology by means of over-expression of natural miRNAs, generation of artificial microRNAs and inhibition of miRNA activity by target mimicry. This review highlights the importance of miRNAs in plant sciences by describing the latest updates in this research field.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
2018-04-13T12:06:18Z
2018-04-13T12:06:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s11105-014-0727-z
http://hdl.handle.net/20.500.12123/2239
0735-9640 (Print)
1572-9818 (Online)
https://doi.org/10.1007/s11105-014-0727-z
url https://link.springer.com/article/10.1007/s11105-014-0727-z
http://hdl.handle.net/20.500.12123/2239
https://doi.org/10.1007/s11105-014-0727-z
identifier_str_mv 0735-9640 (Print)
1572-9818 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Molecular Biology Reporter 32 (6) : 1257–1269 (December 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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