Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived pla...

Autores: Ayala, Lilian P.; Luna, Claudia V.; Brugnoli, Elsa Andrea; Espasandin, Fabiana Daniela; Duarte, María José; González, Ana María; Gauchat, Maria Elena; Moncaleán Guillén, Paloma; Sansberro, Pedro Alfonso
Año de publicación: 2022
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The low seed production of the interspecific hybrid Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis restricts its commercial expansion, making it necessary to ensure efficient cryopreservation and a propagation protocol with no genetic variability. Mature zygotic embryos (MZEs) were cultured in Murashige and Skoog (MS) medium containing 6-benzyladenine (BA) and thidiazuron (TDZ). After 45 days in culture, the highest rate of regeneration (86.7 ± 8.8%) and the maximum number of differentiated buds per responsive explant (15.5 ± 2.8) were achieved from explants cultivated on MS with BA and TDZ (0.5 μM each). Cryopreservation of zygotic embryos using a simple desiccation step and a direct immersion into liquid nitrogen did not affect regeneration and would enhance embryo storage duration. Half-strength MS enriched with sucrose (0.09 M) and gelled with gellan gum (4 g L− 1) under forced ventilation culture was used for shoot elongation. Subsequently, 73 ± 6.7% of shoots produced roots after pretreatment with 1.25 mM indole-3-butyric acid solution for 5 min and culture on quarter-strength MS with sucrose (0.045 M). The regenerated plantlets were successfully transferred to ex vitro conditions. The procedure took 160 days and comprised the adventitious bud formation from cryopreserved MZEs, shoot elongation, rooting, and plantlet acclimation. Considering that water deficit is the major strain during forest establishment, a controlled experiment was carried out to determine the competence of plantlets to overcome this stress. Next, field studies assessed the survival rate and growth of 16-month-old plants. Our results indicated that the field performance of tissue-culture-derived plants is similar to seedlings and rooted cutting plants. Additionally, inter-simple sequence repeat marker analysis revealed the genetic uniformity among the in vitro-raised plants, demonstrating the reliability and validity of the procedure. Thus, the developed regeneration and cryopreservation protocol for mature zygotic embryo explants is a valuable alternative for breeding programs and commercial P. elliottii x P. caribaea propagation.
EEA Montecarlo
Fil: Ayala, Lilian. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Luna, Claudia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Brugnoli, Elsa. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Espasandin, Fabiana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Gauchat, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina
Fil: Moncaleán Guillén, Paloma. Centro de Arkaute, Neiker-BRTA. Departamento de Ciencias Forestales; España
Fil: Sansberro, Pedro. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fuente: Trees Structure and Function : 37 : 417-433. (2023)
Materia: Híbridos
Criopreservación
Organogénesis
Hybrids
Pinus
Cryopreservation
Organogenesis
Pinus elliottii
Pinus caribaea
Híbrido P. elliottii x P. caribaea
P. elliottii x P. caribaea Hybrids
Nivel de accesibilidad: acceso restringido
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/21350

Acceder

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spelling	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plantsAyala, Lilian P.Luna, Claudia V.Brugnoli, Elsa AndreaEspasandin, Fabiana DanielaDuarte, María JoséGonzález, Ana MaríaGauchat, Maria ElenaMoncaleán Guillén, PalomaSansberro, Pedro AlfonsoHíbridosCriopreservaciónOrganogénesisHybridsPinusCryopreservationOrganogenesisPinus elliottiiPinus caribaeaHíbrido P. elliottii x P. caribaeaP. elliottii x P. caribaea HybridsThe low seed production of the interspecific hybrid Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis restricts its commercial expansion, making it necessary to ensure efficient cryopreservation and a propagation protocol with no genetic variability. Mature zygotic embryos (MZEs) were cultured in Murashige and Skoog (MS) medium containing 6-benzyladenine (BA) and thidiazuron (TDZ). After 45 days in culture, the highest rate of regeneration (86.7 ± 8.8%) and the maximum number of differentiated buds per responsive explant (15.5 ± 2.8) were achieved from explants cultivated on MS with BA and TDZ (0.5 μM each). Cryopreservation of zygotic embryos using a simple desiccation step and a direct immersion into liquid nitrogen did not affect regeneration and would enhance embryo storage duration. Half-strength MS enriched with sucrose (0.09 M) and gelled with gellan gum (4 g L− 1) under forced ventilation culture was used for shoot elongation. Subsequently, 73 ± 6.7% of shoots produced roots after pretreatment with 1.25 mM indole-3-butyric acid solution for 5 min and culture on quarter-strength MS with sucrose (0.045 M). The regenerated plantlets were successfully transferred to ex vitro conditions. The procedure took 160 days and comprised the adventitious bud formation from cryopreserved MZEs, shoot elongation, rooting, and plantlet acclimation. Considering that water deficit is the major strain during forest establishment, a controlled experiment was carried out to determine the competence of plantlets to overcome this stress. Next, field studies assessed the survival rate and growth of 16-month-old plants. Our results indicated that the field performance of tissue-culture-derived plants is similar to seedlings and rooted cutting plants. Additionally, inter-simple sequence repeat marker analysis revealed the genetic uniformity among the in vitro-raised plants, demonstrating the reliability and validity of the procedure. Thus, the developed regeneration and cryopreservation protocol for mature zygotic embryo explants is a valuable alternative for breeding programs and commercial P. elliottii x P. caribaea propagation.EEA MontecarloFil: Ayala, Lilian. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Luna, Claudia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Brugnoli, Elsa. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Espasandin, Fabiana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Gauchat, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; ArgentinaFil: Moncaleán Guillén, Paloma. Centro de Arkaute, Neiker-BRTA. Departamento de Ciencias Forestales; EspañaFil: Sansberro, Pedro. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaSpringer Nature2025-02-19T13:02:52Z2025-02-19T13:02:52Z2022-10-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21350https://link.springer.com/article/10.1007/s00468-022-02359-0Ayala, L.P.E., Luna, C.V., Brugnoli, E.A. et al. Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants. Trees 37, 417–433 (2023). https://doi.org/10.1007/s00468-022-02359-01432-2285https://doi.org/10.1007/s00468-022-02359-0Trees Structure and Function : 37 : 417-433. (2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:09Zoai:localhost:20.500.12123/21350instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:09.468INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
title	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
spellingShingle	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants Ayala, Lilian P. Híbridos Criopreservación Organogénesis Hybrids Pinus Cryopreservation Organogenesis Pinus elliottii Pinus caribaea Híbrido P. elliottii x P. caribaea P. elliottii x P. caribaea Hybrids
title_short	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
title_full	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
title_fullStr	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
title_full_unstemmed	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
title_sort	Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants
dc.creator.none.fl_str_mv	Ayala, Lilian P. Luna, Claudia V. Brugnoli, Elsa Andrea Espasandin, Fabiana Daniela Duarte, María José González, Ana María Gauchat, Maria Elena Moncaleán Guillén, Paloma Sansberro, Pedro Alfonso
author	Ayala, Lilian P.
author_facet	Ayala, Lilian P. Luna, Claudia V. Brugnoli, Elsa Andrea Espasandin, Fabiana Daniela Duarte, María José González, Ana María Gauchat, Maria Elena Moncaleán Guillén, Paloma Sansberro, Pedro Alfonso
author_role	author
author2	Luna, Claudia V. Brugnoli, Elsa Andrea Espasandin, Fabiana Daniela Duarte, María José González, Ana María Gauchat, Maria Elena Moncaleán Guillén, Paloma Sansberro, Pedro Alfonso
author2_role	author author author author author author author author
dc.subject.none.fl_str_mv	Híbridos Criopreservación Organogénesis Hybrids Pinus Cryopreservation Organogenesis Pinus elliottii Pinus caribaea Híbrido P. elliottii x P. caribaea P. elliottii x P. caribaea Hybrids
topic	Híbridos Criopreservación Organogénesis Hybrids Pinus Cryopreservation Organogenesis Pinus elliottii Pinus caribaea Híbrido P. elliottii x P. caribaea P. elliottii x P. caribaea Hybrids
dc.description.none.fl_txt_mv	The low seed production of the interspecific hybrid Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis restricts its commercial expansion, making it necessary to ensure efficient cryopreservation and a propagation protocol with no genetic variability. Mature zygotic embryos (MZEs) were cultured in Murashige and Skoog (MS) medium containing 6-benzyladenine (BA) and thidiazuron (TDZ). After 45 days in culture, the highest rate of regeneration (86.7 ± 8.8%) and the maximum number of differentiated buds per responsive explant (15.5 ± 2.8) were achieved from explants cultivated on MS with BA and TDZ (0.5 μM each). Cryopreservation of zygotic embryos using a simple desiccation step and a direct immersion into liquid nitrogen did not affect regeneration and would enhance embryo storage duration. Half-strength MS enriched with sucrose (0.09 M) and gelled with gellan gum (4 g L− 1) under forced ventilation culture was used for shoot elongation. Subsequently, 73 ± 6.7% of shoots produced roots after pretreatment with 1.25 mM indole-3-butyric acid solution for 5 min and culture on quarter-strength MS with sucrose (0.045 M). The regenerated plantlets were successfully transferred to ex vitro conditions. The procedure took 160 days and comprised the adventitious bud formation from cryopreserved MZEs, shoot elongation, rooting, and plantlet acclimation. Considering that water deficit is the major strain during forest establishment, a controlled experiment was carried out to determine the competence of plantlets to overcome this stress. Next, field studies assessed the survival rate and growth of 16-month-old plants. Our results indicated that the field performance of tissue-culture-derived plants is similar to seedlings and rooted cutting plants. Additionally, inter-simple sequence repeat marker analysis revealed the genetic uniformity among the in vitro-raised plants, demonstrating the reliability and validity of the procedure. Thus, the developed regeneration and cryopreservation protocol for mature zygotic embryo explants is a valuable alternative for breeding programs and commercial P. elliottii x P. caribaea propagation. EEA Montecarlo Fil: Ayala, Lilian. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Luna, Claudia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Brugnoli, Elsa. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Espasandin, Fabiana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Gauchat, María E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina Fil: Moncaleán Guillén, Paloma. Centro de Arkaute, Neiker-BRTA. Departamento de Ciencias Forestales; España Fil: Sansberro, Pedro. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
description	The low seed production of the interspecific hybrid Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis restricts its commercial expansion, making it necessary to ensure efficient cryopreservation and a propagation protocol with no genetic variability. Mature zygotic embryos (MZEs) were cultured in Murashige and Skoog (MS) medium containing 6-benzyladenine (BA) and thidiazuron (TDZ). After 45 days in culture, the highest rate of regeneration (86.7 ± 8.8%) and the maximum number of differentiated buds per responsive explant (15.5 ± 2.8) were achieved from explants cultivated on MS with BA and TDZ (0.5 μM each). Cryopreservation of zygotic embryos using a simple desiccation step and a direct immersion into liquid nitrogen did not affect regeneration and would enhance embryo storage duration. Half-strength MS enriched with sucrose (0.09 M) and gelled with gellan gum (4 g L− 1) under forced ventilation culture was used for shoot elongation. Subsequently, 73 ± 6.7% of shoots produced roots after pretreatment with 1.25 mM indole-3-butyric acid solution for 5 min and culture on quarter-strength MS with sucrose (0.045 M). The regenerated plantlets were successfully transferred to ex vitro conditions. The procedure took 160 days and comprised the adventitious bud formation from cryopreserved MZEs, shoot elongation, rooting, and plantlet acclimation. Considering that water deficit is the major strain during forest establishment, a controlled experiment was carried out to determine the competence of plantlets to overcome this stress. Next, field studies assessed the survival rate and growth of 16-month-old plants. Our results indicated that the field performance of tissue-culture-derived plants is similar to seedlings and rooted cutting plants. Additionally, inter-simple sequence repeat marker analysis revealed the genetic uniformity among the in vitro-raised plants, demonstrating the reliability and validity of the procedure. Thus, the developed regeneration and cryopreservation protocol for mature zygotic embryo explants is a valuable alternative for breeding programs and commercial P. elliottii x P. caribaea propagation.
publishDate	2022
dc.date.none.fl_str_mv	2022-10-22 2025-02-19T13:02:52Z 2025-02-19T13:02:52Z
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/21350 https://link.springer.com/article/10.1007/s00468-022-02359-0 Ayala, L.P.E., Luna, C.V., Brugnoli, E.A. et al. Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants. Trees 37, 417–433 (2023). https://doi.org/10.1007/s00468-022-02359-0 1432-2285 https://doi.org/10.1007/s00468-022-02359-0
url	http://hdl.handle.net/20.500.12123/21350 https://link.springer.com/article/10.1007/s00468-022-02359-0 https://doi.org/10.1007/s00468-022-02359-0
identifier_str_mv	Ayala, L.P.E., Luna, C.V., Brugnoli, E.A. et al. Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived plants. Trees 37, 417–433 (2023). https://doi.org/10.1007/s00468-022-02359-0 1432-2285
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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dc.publisher.none.fl_str_mv	Springer Nature
publisher.none.fl_str_mv	Springer Nature
dc.source.none.fl_str_mv	Trees Structure and Function : 37 : 417-433. (2023) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
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repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Cryopreservation of mature zygotic embryos, shoot bud regeneration, and field establishment of Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis in vitro-derived pla...

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