Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine

Autores
Barandiaran, Soledad; Perez Aguirreburualde, María Sol; Marfil, María Jimena; Martinez Vivot, Marcela; Aznar, Maria Natalia; Zumarraga, Martin Jose; Pérez, Andrés M.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infection with the Mycobacterium bovis (M. bovis) causes a disease referred to as bovine tuberculosis (bTB), which affects a wide range of mammal hosts. Many countries have implemented control and eradication plans that have resulted in variable levels of efficacy and success. Although bTB is a notifiable disease in Argentina, and a control plan that targets cattle herds has been in place for decades, M. bovis is still prevalent in cattle, swine, and certain wild species. The aim of the paper here was to assess the sensitivity (Se), specificity (Sp), and positive and negative predictive values (PPV and NPV) of PCR from tissue, which is a test for rapid M. bovis detection in swine. Bacteriological culture was also performed for comparison purposes. A Bayesian approach was applied to estimate the accuracy of the diagnostic tests, PCR and bacteriological culture, in 266 swine samples with bTB-like lesions recovered during routine official inspections at slaughterhouses. A one-population model, assuming conditional dependence between test results, and incorporating prior information on the performance of the tests obtained from the literature, was used to estimate the tests Se and Sp. The accuracy of the combined (in parallel) application of both tests was also estimated. The Se of the PCR (82.9%) was higher than the Se of the bacteriological culture (79.9%), whereas the Sp of both tests was similar (88.5 and 89.0%, respectively). Furthermore, when both techniques were assessed in parallel, the Se of the diagnostic system increased substantially (Se = 96.6%) with a moderate Sp loss (Sp = 78.8%; PPV = 92.8%; NPV = 89%). Results suggest that the PCR, or the combined application of bacteriological culture and PCR, may serve as an accurate diagnostic tool to confirm bTB in swine samples. Results here will help the design and implementation of effective surveillance strategies for the disease in swine of Argentina and other settings in which the disease is prevalent.
Instituto de Patobiología
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Aguirreburualde, María Sol. University of Minnesota. College of Veterinary Medicine; Estados Unidos
Fil: Marfil, María Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Aznar, Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Andrés M. University of Minnesota. College of Veterinary Medicine; Estados Unidos
Fuente
Frontiers in Veterinary Science 6 : 204 (Junio 2019)
Materia
PCR
Técnicas de Diagnosis
Tuberculosis
Cerdo
Diagnostic Techniques
Swine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swineBarandiaran, SoledadPerez Aguirreburualde, María SolMarfil, María JimenaMartinez Vivot, MarcelaAznar, Maria NataliaZumarraga, Martin JosePérez, Andrés M.PCRTécnicas de DiagnosisTuberculosisCerdoDiagnostic TechniquesSwineInfection with the Mycobacterium bovis (M. bovis) causes a disease referred to as bovine tuberculosis (bTB), which affects a wide range of mammal hosts. Many countries have implemented control and eradication plans that have resulted in variable levels of efficacy and success. Although bTB is a notifiable disease in Argentina, and a control plan that targets cattle herds has been in place for decades, M. bovis is still prevalent in cattle, swine, and certain wild species. The aim of the paper here was to assess the sensitivity (Se), specificity (Sp), and positive and negative predictive values (PPV and NPV) of PCR from tissue, which is a test for rapid M. bovis detection in swine. Bacteriological culture was also performed for comparison purposes. A Bayesian approach was applied to estimate the accuracy of the diagnostic tests, PCR and bacteriological culture, in 266 swine samples with bTB-like lesions recovered during routine official inspections at slaughterhouses. A one-population model, assuming conditional dependence between test results, and incorporating prior information on the performance of the tests obtained from the literature, was used to estimate the tests Se and Sp. The accuracy of the combined (in parallel) application of both tests was also estimated. The Se of the PCR (82.9%) was higher than the Se of the bacteriological culture (79.9%), whereas the Sp of both tests was similar (88.5 and 89.0%, respectively). Furthermore, when both techniques were assessed in parallel, the Se of the diagnostic system increased substantially (Se = 96.6%) with a moderate Sp loss (Sp = 78.8%; PPV = 92.8%; NPV = 89%). Results suggest that the PCR, or the combined application of bacteriological culture and PCR, may serve as an accurate diagnostic tool to confirm bTB in swine samples. Results here will help the design and implementation of effective surveillance strategies for the disease in swine of Argentina and other settings in which the disease is prevalent.Instituto de PatobiologíaFil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Aguirreburualde, María Sol. University of Minnesota. College of Veterinary Medicine; Estados UnidosFil: Marfil, María Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Aznar, Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, Andrés M. University of Minnesota. College of Veterinary Medicine; Estados UnidosFrontiers Media2020-10-29T17:29:57Z2020-10-29T17:29:57Z2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8155https://www.frontiersin.org/articles/10.3389/fvets.2019.00204/full2297-1769https://doi.org/10.3389/fvets.2019.00204Frontiers in Veterinary Science 6 : 204 (Junio 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:03Zoai:localhost:20.500.12123/8155instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:03.538INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
title Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
spellingShingle Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
Barandiaran, Soledad
PCR
Técnicas de Diagnosis
Tuberculosis
Cerdo
Diagnostic Techniques
Swine
title_short Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
title_full Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
title_fullStr Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
title_full_unstemmed Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
title_sort Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
dc.creator.none.fl_str_mv Barandiaran, Soledad
Perez Aguirreburualde, María Sol
Marfil, María Jimena
Martinez Vivot, Marcela
Aznar, Maria Natalia
Zumarraga, Martin Jose
Pérez, Andrés M.
author Barandiaran, Soledad
author_facet Barandiaran, Soledad
Perez Aguirreburualde, María Sol
Marfil, María Jimena
Martinez Vivot, Marcela
Aznar, Maria Natalia
Zumarraga, Martin Jose
Pérez, Andrés M.
author_role author
author2 Perez Aguirreburualde, María Sol
Marfil, María Jimena
Martinez Vivot, Marcela
Aznar, Maria Natalia
Zumarraga, Martin Jose
Pérez, Andrés M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PCR
Técnicas de Diagnosis
Tuberculosis
Cerdo
Diagnostic Techniques
Swine
topic PCR
Técnicas de Diagnosis
Tuberculosis
Cerdo
Diagnostic Techniques
Swine
dc.description.none.fl_txt_mv Infection with the Mycobacterium bovis (M. bovis) causes a disease referred to as bovine tuberculosis (bTB), which affects a wide range of mammal hosts. Many countries have implemented control and eradication plans that have resulted in variable levels of efficacy and success. Although bTB is a notifiable disease in Argentina, and a control plan that targets cattle herds has been in place for decades, M. bovis is still prevalent in cattle, swine, and certain wild species. The aim of the paper here was to assess the sensitivity (Se), specificity (Sp), and positive and negative predictive values (PPV and NPV) of PCR from tissue, which is a test for rapid M. bovis detection in swine. Bacteriological culture was also performed for comparison purposes. A Bayesian approach was applied to estimate the accuracy of the diagnostic tests, PCR and bacteriological culture, in 266 swine samples with bTB-like lesions recovered during routine official inspections at slaughterhouses. A one-population model, assuming conditional dependence between test results, and incorporating prior information on the performance of the tests obtained from the literature, was used to estimate the tests Se and Sp. The accuracy of the combined (in parallel) application of both tests was also estimated. The Se of the PCR (82.9%) was higher than the Se of the bacteriological culture (79.9%), whereas the Sp of both tests was similar (88.5 and 89.0%, respectively). Furthermore, when both techniques were assessed in parallel, the Se of the diagnostic system increased substantially (Se = 96.6%) with a moderate Sp loss (Sp = 78.8%; PPV = 92.8%; NPV = 89%). Results suggest that the PCR, or the combined application of bacteriological culture and PCR, may serve as an accurate diagnostic tool to confirm bTB in swine samples. Results here will help the design and implementation of effective surveillance strategies for the disease in swine of Argentina and other settings in which the disease is prevalent.
Instituto de Patobiología
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Aguirreburualde, María Sol. University of Minnesota. College of Veterinary Medicine; Estados Unidos
Fil: Marfil, María Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Aznar, Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Andrés M. University of Minnesota. College of Veterinary Medicine; Estados Unidos
description Infection with the Mycobacterium bovis (M. bovis) causes a disease referred to as bovine tuberculosis (bTB), which affects a wide range of mammal hosts. Many countries have implemented control and eradication plans that have resulted in variable levels of efficacy and success. Although bTB is a notifiable disease in Argentina, and a control plan that targets cattle herds has been in place for decades, M. bovis is still prevalent in cattle, swine, and certain wild species. The aim of the paper here was to assess the sensitivity (Se), specificity (Sp), and positive and negative predictive values (PPV and NPV) of PCR from tissue, which is a test for rapid M. bovis detection in swine. Bacteriological culture was also performed for comparison purposes. A Bayesian approach was applied to estimate the accuracy of the diagnostic tests, PCR and bacteriological culture, in 266 swine samples with bTB-like lesions recovered during routine official inspections at slaughterhouses. A one-population model, assuming conditional dependence between test results, and incorporating prior information on the performance of the tests obtained from the literature, was used to estimate the tests Se and Sp. The accuracy of the combined (in parallel) application of both tests was also estimated. The Se of the PCR (82.9%) was higher than the Se of the bacteriological culture (79.9%), whereas the Sp of both tests was similar (88.5 and 89.0%, respectively). Furthermore, when both techniques were assessed in parallel, the Se of the diagnostic system increased substantially (Se = 96.6%) with a moderate Sp loss (Sp = 78.8%; PPV = 92.8%; NPV = 89%). Results suggest that the PCR, or the combined application of bacteriological culture and PCR, may serve as an accurate diagnostic tool to confirm bTB in swine samples. Results here will help the design and implementation of effective surveillance strategies for the disease in swine of Argentina and other settings in which the disease is prevalent.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
2020-10-29T17:29:57Z
2020-10-29T17:29:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8155
https://www.frontiersin.org/articles/10.3389/fvets.2019.00204/full
2297-1769
https://doi.org/10.3389/fvets.2019.00204
url http://hdl.handle.net/20.500.12123/8155
https://www.frontiersin.org/articles/10.3389/fvets.2019.00204/full
https://doi.org/10.3389/fvets.2019.00204
identifier_str_mv 2297-1769
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Veterinary Science 6 : 204 (Junio 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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