Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection

Autores
Zumarraga, Martin Jose; Meikle, Virginia; Bernardelli, Amelia; Abdala, Alejandro Ariel; Tarabla, Hector Dante; Romano, Maria Isabel; Cataldi, Angel Adrian
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.
Instituto de Biotecnología
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bernardelli, Amelia. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios y Control Técnico; Argentina
Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Journal of Veterinary Diagnostic Investigation 17 (3) : 232-238 (Mayo 2005)
Materia
Mycobacterium bovis
PCR
Técnicas de Diagnosis
Diagnostic Techniques
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8231
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spelling Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detectionZumarraga, Martin JoseMeikle, VirginiaBernardelli, AmeliaAbdala, Alejandro ArielTarabla, Hector DanteRomano, Maria IsabelCataldi, Angel AdrianMycobacterium bovisPCRTécnicas de DiagnosisDiagnostic TechniquesThe confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.Instituto de BiotecnologíaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Bernardelli, Amelia. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios y Control Técnico; ArgentinaFil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaSage Publications2020-11-10T14:55:13Z2020-11-10T14:55:13Z2005-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8231https://journals.sagepub.com/doi/10.1177/1040638705017003031943-4936https://doi.org/10.1177/104063870501700303Journal of Veterinary Diagnostic Investigation 17 (3) : 232-238 (Mayo 2005)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:40Zoai:localhost:20.500.12123/8231instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:42.263INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
title Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
spellingShingle Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
Zumarraga, Martin Jose
Mycobacterium bovis
PCR
Técnicas de Diagnosis
Diagnostic Techniques
title_short Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
title_full Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
title_fullStr Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
title_full_unstemmed Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
title_sort Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
dc.creator.none.fl_str_mv Zumarraga, Martin Jose
Meikle, Virginia
Bernardelli, Amelia
Abdala, Alejandro Ariel
Tarabla, Hector Dante
Romano, Maria Isabel
Cataldi, Angel Adrian
author Zumarraga, Martin Jose
author_facet Zumarraga, Martin Jose
Meikle, Virginia
Bernardelli, Amelia
Abdala, Alejandro Ariel
Tarabla, Hector Dante
Romano, Maria Isabel
Cataldi, Angel Adrian
author_role author
author2 Meikle, Virginia
Bernardelli, Amelia
Abdala, Alejandro Ariel
Tarabla, Hector Dante
Romano, Maria Isabel
Cataldi, Angel Adrian
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium bovis
PCR
Técnicas de Diagnosis
Diagnostic Techniques
topic Mycobacterium bovis
PCR
Técnicas de Diagnosis
Diagnostic Techniques
dc.description.none.fl_txt_mv The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.
Instituto de Biotecnología
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bernardelli, Amelia. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios y Control Técnico; Argentina
Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.
publishDate 2005
dc.date.none.fl_str_mv 2005-05
2020-11-10T14:55:13Z
2020-11-10T14:55:13Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8231
https://journals.sagepub.com/doi/10.1177/104063870501700303
1943-4936
https://doi.org/10.1177/104063870501700303
url http://hdl.handle.net/20.500.12123/8231
https://journals.sagepub.com/doi/10.1177/104063870501700303
https://doi.org/10.1177/104063870501700303
identifier_str_mv 1943-4936
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sage Publications
publisher.none.fl_str_mv Sage Publications
dc.source.none.fl_str_mv Journal of Veterinary Diagnostic Investigation 17 (3) : 232-238 (Mayo 2005)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341382449004544
score 12.623145

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