Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides p...

Autores
Cosson, Patrick; Hastoy, Cécile; Erazzu, Luis Ernesto; Budeguer, Carlos Jorge; Boutié, Philippe; Rolin, Dominique; Schurdi-Levraud, Valérie
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1) germplasm collection and breeding, (2) studies on genetic diversity and its structuring, (3) genomic tools. Results: In this study, we developped 18 EST-SSR from 150,258 EST from The Compositae Genome Project of UC Davis (http://compgenomics.ucdavis.edu/data/). We genotyped 145 S. rebaudiana individuals, issued from thirty-one cultivars and thirty-one landraces of various origins worldwide. Markers polymorphic information content (PIC) ranged between 0.60 and 0.84. An average of 12 alleles per locus and a high observed heterozygoty of 0.69 could be observed. The landraces revealed twice as many private alleles as cultivars. The genotypes could be clustered into 3 genetic populations. The landraces were grouped in the same cluster in which the oldest cultivars “Eirete” and “MoritaIII” type are also found. The other two clusters only include cultivated genotypes. One of them revealed an original genetic variability. SG phenotypes could not discriminate the three genetic clusters but phenotyping showed a wide range of composition in terms of bitter to sweet SGs. Conclusion: This is the first study of genetic diversity in Stevia rebaudiana involving 145 genotypes, including known cultivars as well as landrace populations of different origin. This study pointed out the structuration of S. rebaudiana germplasm and the resource of the landrace populations for genetic improvement, even on the trait of SG’s composition.
EEA Famaillá
Fil: Cosson, Patrick. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia
Fil: Hastoy, Cécile. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia. Oviatis SA; Francia
Fil: Erazzu, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Budeguer, Carlos Jorge. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Boutié, Philippe. Oviatis SA; Francia
Fil: Rolin, Dominique. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia
Fil: Schurdi-Levraud, Valérie. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia
Fuente
BMC Plant Biology 19 : Article number 436 (October 2019)
Materia
Stevia rebaudiana
Genética
Diversidad Genética (como Recurso)
Germoplasma
Glicosidos
Genotipos
Genetics
Genetic Diversity (as Resource)
Germplasm
Glycosides
Genotypes
Yerba Dulce
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6249
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6249
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotypingCosson, PatrickHastoy, CécileErazzu, Luis ErnestoBudeguer, Carlos JorgeBoutié, PhilippeRolin, DominiqueSchurdi-Levraud, ValérieStevia rebaudianaGenéticaDiversidad Genética (como Recurso)GermoplasmaGlicosidosGenotiposGeneticsGenetic Diversity (as Resource)GermplasmGlycosidesGenotypesYerba DulceBackground: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1) germplasm collection and breeding, (2) studies on genetic diversity and its structuring, (3) genomic tools. Results: In this study, we developped 18 EST-SSR from 150,258 EST from The Compositae Genome Project of UC Davis (http://compgenomics.ucdavis.edu/data/). We genotyped 145 S. rebaudiana individuals, issued from thirty-one cultivars and thirty-one landraces of various origins worldwide. Markers polymorphic information content (PIC) ranged between 0.60 and 0.84. An average of 12 alleles per locus and a high observed heterozygoty of 0.69 could be observed. The landraces revealed twice as many private alleles as cultivars. The genotypes could be clustered into 3 genetic populations. The landraces were grouped in the same cluster in which the oldest cultivars “Eirete” and “MoritaIII” type are also found. The other two clusters only include cultivated genotypes. One of them revealed an original genetic variability. SG phenotypes could not discriminate the three genetic clusters but phenotyping showed a wide range of composition in terms of bitter to sweet SGs. Conclusion: This is the first study of genetic diversity in Stevia rebaudiana involving 145 genotypes, including known cultivars as well as landrace populations of different origin. This study pointed out the structuration of S. rebaudiana germplasm and the resource of the landrace populations for genetic improvement, even on the trait of SG’s composition.EEA FamailláFil: Cosson, Patrick. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; FranciaFil: Hastoy, Cécile. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia. Oviatis SA; FranciaFil: Erazzu, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Budeguer, Carlos Jorge. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Boutié, Philippe. Oviatis SA; FranciaFil: Rolin, Dominique. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; FranciaFil: Schurdi-Levraud, Valérie. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; FranciaBMC2019-10-31T12:16:10Z2019-10-31T12:16:10Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2061-yhttp://hdl.handle.net/20.500.12123/62491471-2229https://doi.org/10.1186/s12870-019-2061-yBMC Plant Biology 19 : Article number 436 (October 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:14Zoai:localhost:20.500.12123/6249instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:15.564INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
title Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
spellingShingle Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
Cosson, Patrick
Stevia rebaudiana
Genética
Diversidad Genética (como Recurso)
Germoplasma
Glicosidos
Genotipos
Genetics
Genetic Diversity (as Resource)
Germplasm
Glycosides
Genotypes
Yerba Dulce
title_short Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
title_full Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
title_fullStr Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
title_full_unstemmed Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
title_sort Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
dc.creator.none.fl_str_mv Cosson, Patrick
Hastoy, Cécile
Erazzu, Luis Ernesto
Budeguer, Carlos Jorge
Boutié, Philippe
Rolin, Dominique
Schurdi-Levraud, Valérie
author Cosson, Patrick
author_facet Cosson, Patrick
Hastoy, Cécile
Erazzu, Luis Ernesto
Budeguer, Carlos Jorge
Boutié, Philippe
Rolin, Dominique
Schurdi-Levraud, Valérie
author_role author
author2 Hastoy, Cécile
Erazzu, Luis Ernesto
Budeguer, Carlos Jorge
Boutié, Philippe
Rolin, Dominique
Schurdi-Levraud, Valérie
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Stevia rebaudiana
Genética
Diversidad Genética (como Recurso)
Germoplasma
Glicosidos
Genotipos
Genetics
Genetic Diversity (as Resource)
Germplasm
Glycosides
Genotypes
Yerba Dulce
topic Stevia rebaudiana
Genética
Diversidad Genética (como Recurso)
Germoplasma
Glicosidos
Genotipos
Genetics
Genetic Diversity (as Resource)
Germplasm
Glycosides
Genotypes
Yerba Dulce
dc.description.none.fl_txt_mv Background: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1) germplasm collection and breeding, (2) studies on genetic diversity and its structuring, (3) genomic tools. Results: In this study, we developped 18 EST-SSR from 150,258 EST from The Compositae Genome Project of UC Davis (http://compgenomics.ucdavis.edu/data/). We genotyped 145 S. rebaudiana individuals, issued from thirty-one cultivars and thirty-one landraces of various origins worldwide. Markers polymorphic information content (PIC) ranged between 0.60 and 0.84. An average of 12 alleles per locus and a high observed heterozygoty of 0.69 could be observed. The landraces revealed twice as many private alleles as cultivars. The genotypes could be clustered into 3 genetic populations. The landraces were grouped in the same cluster in which the oldest cultivars “Eirete” and “MoritaIII” type are also found. The other two clusters only include cultivated genotypes. One of them revealed an original genetic variability. SG phenotypes could not discriminate the three genetic clusters but phenotyping showed a wide range of composition in terms of bitter to sweet SGs. Conclusion: This is the first study of genetic diversity in Stevia rebaudiana involving 145 genotypes, including known cultivars as well as landrace populations of different origin. This study pointed out the structuration of S. rebaudiana germplasm and the resource of the landrace populations for genetic improvement, even on the trait of SG’s composition.
EEA Famaillá
Fil: Cosson, Patrick. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia
Fil: Hastoy, Cécile. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia. Oviatis SA; Francia
Fil: Erazzu, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Budeguer, Carlos Jorge. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Boutié, Philippe. Oviatis SA; Francia
Fil: Rolin, Dominique. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia
Fil: Schurdi-Levraud, Valérie. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia
description Background: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1) germplasm collection and breeding, (2) studies on genetic diversity and its structuring, (3) genomic tools. Results: In this study, we developped 18 EST-SSR from 150,258 EST from The Compositae Genome Project of UC Davis (http://compgenomics.ucdavis.edu/data/). We genotyped 145 S. rebaudiana individuals, issued from thirty-one cultivars and thirty-one landraces of various origins worldwide. Markers polymorphic information content (PIC) ranged between 0.60 and 0.84. An average of 12 alleles per locus and a high observed heterozygoty of 0.69 could be observed. The landraces revealed twice as many private alleles as cultivars. The genotypes could be clustered into 3 genetic populations. The landraces were grouped in the same cluster in which the oldest cultivars “Eirete” and “MoritaIII” type are also found. The other two clusters only include cultivated genotypes. One of them revealed an original genetic variability. SG phenotypes could not discriminate the three genetic clusters but phenotyping showed a wide range of composition in terms of bitter to sweet SGs. Conclusion: This is the first study of genetic diversity in Stevia rebaudiana involving 145 genotypes, including known cultivars as well as landrace populations of different origin. This study pointed out the structuration of S. rebaudiana germplasm and the resource of the landrace populations for genetic improvement, even on the trait of SG’s composition.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-31T12:16:10Z
2019-10-31T12:16:10Z
2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2061-y
http://hdl.handle.net/20.500.12123/6249
1471-2229
https://doi.org/10.1186/s12870-019-2061-y
url https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2061-y
http://hdl.handle.net/20.500.12123/6249
https://doi.org/10.1186/s12870-019-2061-y
identifier_str_mv 1471-2229
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv BMC Plant Biology 19 : Article number 436 (October 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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