First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
- Autores
- Cabrera Mederos, Dariel; Trucco, Veronica Milagros; Bejerman, Nicolas Esteban; Lenardon, Sergio Luis; Giolitti, Fabian
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina - Fuente
- Plant Disease 103 (12) : 3290 (December 2019)
- Materia
-
Helianthus Annuus
Virus de las Plantas
Argentina
Plant Viruses
Tobacco Streak Virus
Sunflower
Girasol - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8096
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First Report of Tobacco Streak Virus Infecting Sunflower in ArgentinaCabrera Mederos, DarielTrucco, Veronica MilagrosBejerman, Nicolas EstebanLenardon, Sergio LuisGiolitti, FabianHelianthus AnnuusVirus de las PlantasArgentinaPlant VirusesTobacco Streak VirusSunflowerGirasolSunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directionsInstituto de Patología VegetalFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaAmerican Phytopathological Society2020-10-21T16:41:30Z2020-10-21T16:41:30Z2019-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDNhttp://hdl.handle.net/20.500.12123/80960191-29171943-7692https://doi.org/10.1094/PDIS-04-19-0849-PDNPlant Disease 103 (12) : 3290 (December 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:03Zoai:localhost:20.500.12123/8096instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:03.342INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
spellingShingle |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina Cabrera Mederos, Dariel Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol |
title_short |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_full |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_fullStr |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_full_unstemmed |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_sort |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
dc.creator.none.fl_str_mv |
Cabrera Mederos, Dariel Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author |
Cabrera Mederos, Dariel |
author_facet |
Cabrera Mederos, Dariel Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author_role |
author |
author2 |
Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol |
topic |
Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol |
dc.description.none.fl_txt_mv |
Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions Instituto de Patología Vegetal Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina |
description |
Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-21 2020-10-21T16:41:30Z 2020-10-21T16:41:30Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN http://hdl.handle.net/20.500.12123/8096 0191-2917 1943-7692 https://doi.org/10.1094/PDIS-04-19-0849-PDN |
url |
https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN http://hdl.handle.net/20.500.12123/8096 https://doi.org/10.1094/PDIS-04-19-0849-PDN |
identifier_str_mv |
0191-2917 1943-7692 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Phytopathological Society |
publisher.none.fl_str_mv |
American Phytopathological Society |
dc.source.none.fl_str_mv |
Plant Disease 103 (12) : 3290 (December 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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