First Report of Tobacco Streak Virus Infecting Sunflower in Argentina

Autores
Cabrera Mederos, Dariel; Trucco, Veronica Milagros; Bejerman, Nicolas Esteban; Lenardon, Sergio Luis; Giolitti, Fabian
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fuente
Plant Disease 103 (12) : 3290 (December 2019)
Materia
Helianthus Annuus
Virus de las Plantas
Argentina
Plant Viruses
Tobacco Streak Virus
Sunflower
Girasol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8096
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8096
network_acronym_str INTADig
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spelling First Report of Tobacco Streak Virus Infecting Sunflower in ArgentinaCabrera Mederos, DarielTrucco, Veronica MilagrosBejerman, Nicolas EstebanLenardon, Sergio LuisGiolitti, FabianHelianthus AnnuusVirus de las PlantasArgentinaPlant VirusesTobacco Streak VirusSunflowerGirasolSunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directionsInstituto de Patología VegetalFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; ArgentinaAmerican Phytopathological Society2020-10-21T16:41:30Z2020-10-21T16:41:30Z2019-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDNhttp://hdl.handle.net/20.500.12123/80960191-29171943-7692https://doi.org/10.1094/PDIS-04-19-0849-PDNPlant Disease 103 (12) : 3290 (December 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-30T11:23:30Zoai:localhost:20.500.12123/8096instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-30 11:23:30.558INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
title First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
spellingShingle First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
Cabrera Mederos, Dariel
Helianthus Annuus
Virus de las Plantas
Argentina
Plant Viruses
Tobacco Streak Virus
Sunflower
Girasol
title_short First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
title_full First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
title_fullStr First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
title_full_unstemmed First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
title_sort First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
dc.creator.none.fl_str_mv Cabrera Mederos, Dariel
Trucco, Veronica Milagros
Bejerman, Nicolas Esteban
Lenardon, Sergio Luis
Giolitti, Fabian
author Cabrera Mederos, Dariel
author_facet Cabrera Mederos, Dariel
Trucco, Veronica Milagros
Bejerman, Nicolas Esteban
Lenardon, Sergio Luis
Giolitti, Fabian
author_role author
author2 Trucco, Veronica Milagros
Bejerman, Nicolas Esteban
Lenardon, Sergio Luis
Giolitti, Fabian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Helianthus Annuus
Virus de las Plantas
Argentina
Plant Viruses
Tobacco Streak Virus
Sunflower
Girasol
topic Helianthus Annuus
Virus de las Plantas
Argentina
Plant Viruses
Tobacco Streak Virus
Sunflower
Girasol
dc.description.none.fl_txt_mv Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina
description Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions
publishDate 2019
dc.date.none.fl_str_mv 2019-11-21
2020-10-21T16:41:30Z
2020-10-21T16:41:30Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN
http://hdl.handle.net/20.500.12123/8096
0191-2917
1943-7692
https://doi.org/10.1094/PDIS-04-19-0849-PDN
url https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN
http://hdl.handle.net/20.500.12123/8096
https://doi.org/10.1094/PDIS-04-19-0849-PDN
identifier_str_mv 0191-2917
1943-7692
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Plant Disease 103 (12) : 3290 (December 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.589754

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