Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)

Autores
Bejerman, Nicolas Esteban; Giolitti, Fabian; De Breuil, Soledad; Lenardon, Sergio Luis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
Instituto de Patología Vegetal
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fuente
Archives of virology 158 (2) : 485–490. (February 2013)
Materia
Helianthus Annuus
Virus de las Plantas
Clones
Potyvirus
Inoculación
Plant Viruses
Potyviruses
Inoculation
Girasol
Virus del Moteado Clorótico del Girasol
Sunflower
SuCMoV
Sunflower Chlorotic Mottle Virus
Infectious Clone
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3711
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3711
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)Bejerman, Nicolas EstebanGiolitti, FabianDe Breuil, SoledadLenardon, Sergio LuisHelianthus AnnuusVirus de las PlantasClonesPotyvirusInoculaciónPlant VirusesPotyvirusesInoculationGirasolVirus del Moteado Clorótico del GirasolSunflowerSuCMoVSunflower Chlorotic Mottle VirusInfectious CloneA full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.Instituto de Patología VegetalFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaSpringer; Virology Division, International Union of Microbiological Societies2018-10-25T15:25:00Z2018-10-25T15:25:00Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3711https://link.springer.com/article/10.1007/s00705-012-1497-20304-86081432-8798 (Online)10.1007/s00705-012-1497-2Archives of virology 158 (2) : 485–490. (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:28Zoai:localhost:20.500.12123/3711instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:29.131INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
title Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
spellingShingle Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
Bejerman, Nicolas Esteban
Helianthus Annuus
Virus de las Plantas
Clones
Potyvirus
Inoculación
Plant Viruses
Potyviruses
Inoculation
Girasol
Virus del Moteado Clorótico del Girasol
Sunflower
SuCMoV
Sunflower Chlorotic Mottle Virus
Infectious Clone
title_short Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
title_full Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
title_fullStr Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
title_full_unstemmed Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
title_sort Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
dc.creator.none.fl_str_mv Bejerman, Nicolas Esteban
Giolitti, Fabian
De Breuil, Soledad
Lenardon, Sergio Luis
author Bejerman, Nicolas Esteban
author_facet Bejerman, Nicolas Esteban
Giolitti, Fabian
De Breuil, Soledad
Lenardon, Sergio Luis
author_role author
author2 Giolitti, Fabian
De Breuil, Soledad
Lenardon, Sergio Luis
author2_role author
author
author
dc.subject.none.fl_str_mv Helianthus Annuus
Virus de las Plantas
Clones
Potyvirus
Inoculación
Plant Viruses
Potyviruses
Inoculation
Girasol
Virus del Moteado Clorótico del Girasol
Sunflower
SuCMoV
Sunflower Chlorotic Mottle Virus
Infectious Clone
topic Helianthus Annuus
Virus de las Plantas
Clones
Potyvirus
Inoculación
Plant Viruses
Potyviruses
Inoculation
Girasol
Virus del Moteado Clorótico del Girasol
Sunflower
SuCMoV
Sunflower Chlorotic Mottle Virus
Infectious Clone
dc.description.none.fl_txt_mv A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
Instituto de Patología Vegetal
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
description A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2018-10-25T15:25:00Z
2018-10-25T15:25:00Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3711
https://link.springer.com/article/10.1007/s00705-012-1497-2
0304-8608
1432-8798 (Online)
10.1007/s00705-012-1497-2
url http://hdl.handle.net/20.500.12123/3711
https://link.springer.com/article/10.1007/s00705-012-1497-2
identifier_str_mv 0304-8608
1432-8798 (Online)
10.1007/s00705-012-1497-2
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer; Virology Division, International Union of Microbiological Societies
publisher.none.fl_str_mv Springer; Virology Division, International Union of Microbiological Societies
dc.source.none.fl_str_mv Archives of virology 158 (2) : 485–490. (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619127318118400
score 12.559606

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