Development and assessment of a new cage‐like particle adjuvant

Autores
Bertona, Daiana; Pujato, Nazarena; Bontempi, Iván; González, Verónica Doris Guadalupe; Cabrera, Gabriel Gustavo; Gugliotta, Luis Marcelino; Hozbor, Daniela Flavia; Nicastro, Alcides; Calvinho, Luis Fernando; Marcipar, Iván Sergio
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.
EEA Rafaela
Fil: Bertona, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Bontempi, Iván. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Cabrera, Gabriel Gustavo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Hozbor, Daniela Flavia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nicastro, Alcides. LIPOMIZE SRL Santa Fe; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Pharmacy and Pharmacology 69 (10) : 1293-1303 (October 2017)
Materia
Ganado Bovino
Trypanosoma cruzi
Coadyuvantes
Respuesta Inmunológica
Cattle
Adjuvants
Immune Response
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2813
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2813
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network_name_str INTA Digital (INTA)
spelling Development and assessment of a new cage‐like particle adjuvantBertona, DaianaPujato, NazarenaBontempi, IvánGonzález, Verónica Doris GuadalupeCabrera, Gabriel GustavoGugliotta, Luis MarcelinoHozbor, Daniela FlaviaNicastro, AlcidesCalvinho, Luis FernandoMarcipar, Iván SergioGanado BovinoTrypanosoma cruziCoadyuvantesRespuesta InmunológicaCattleAdjuvantsImmune ResponseObjectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.EEA RafaelaFil: Bertona, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Bontempi, Iván. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Cabrera, Gabriel Gustavo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Hozbor, Daniela Flavia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nicastro, Alcides. LIPOMIZE SRL Santa Fe; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-07-18T14:14:26Z2018-07-18T14:14:26Z2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768http://hdl.handle.net/20.500.12123/28130022-35732042-7158https://doi.org/10.1111/jphp.12768Journal of Pharmacy and Pharmacology 69 (10) : 1293-1303 (October 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-06T09:39:36Zoai:localhost:20.500.12123/2813instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:39:37.573INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and assessment of a new cage‐like particle adjuvant
title Development and assessment of a new cage‐like particle adjuvant
spellingShingle Development and assessment of a new cage‐like particle adjuvant
Bertona, Daiana
Ganado Bovino
Trypanosoma cruzi
Coadyuvantes
Respuesta Inmunológica
Cattle
Adjuvants
Immune Response
title_short Development and assessment of a new cage‐like particle adjuvant
title_full Development and assessment of a new cage‐like particle adjuvant
title_fullStr Development and assessment of a new cage‐like particle adjuvant
title_full_unstemmed Development and assessment of a new cage‐like particle adjuvant
title_sort Development and assessment of a new cage‐like particle adjuvant
dc.creator.none.fl_str_mv Bertona, Daiana
Pujato, Nazarena
Bontempi, Iván
González, Verónica Doris Guadalupe
Cabrera, Gabriel Gustavo
Gugliotta, Luis Marcelino
Hozbor, Daniela Flavia
Nicastro, Alcides
Calvinho, Luis Fernando
Marcipar, Iván Sergio
author Bertona, Daiana
author_facet Bertona, Daiana
Pujato, Nazarena
Bontempi, Iván
González, Verónica Doris Guadalupe
Cabrera, Gabriel Gustavo
Gugliotta, Luis Marcelino
Hozbor, Daniela Flavia
Nicastro, Alcides
Calvinho, Luis Fernando
Marcipar, Iván Sergio
author_role author
author2 Pujato, Nazarena
Bontempi, Iván
González, Verónica Doris Guadalupe
Cabrera, Gabriel Gustavo
Gugliotta, Luis Marcelino
Hozbor, Daniela Flavia
Nicastro, Alcides
Calvinho, Luis Fernando
Marcipar, Iván Sergio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Trypanosoma cruzi
Coadyuvantes
Respuesta Inmunológica
Cattle
Adjuvants
Immune Response
topic Ganado Bovino
Trypanosoma cruzi
Coadyuvantes
Respuesta Inmunológica
Cattle
Adjuvants
Immune Response
dc.description.none.fl_txt_mv Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.
EEA Rafaela
Fil: Bertona, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Bontempi, Iván. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Cabrera, Gabriel Gustavo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Hozbor, Daniela Flavia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nicastro, Alcides. LIPOMIZE SRL Santa Fe; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
2018-07-18T14:14:26Z
2018-07-18T14:14:26Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768
http://hdl.handle.net/20.500.12123/2813
0022-3573
2042-7158
https://doi.org/10.1111/jphp.12768
url https://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768
http://hdl.handle.net/20.500.12123/2813
https://doi.org/10.1111/jphp.12768
identifier_str_mv 0022-3573
2042-7158
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Pharmacy and Pharmacology 69 (10) : 1293-1303 (October 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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