Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant

Autores
Camussone, Cecilia; Veaute, Carolina Melania Isabel; Pujato, Nazarena; Morein, Bror; Marcipar, Iván Sergio; Calvinho, Luis Fernando
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine.
EEA Rafaela
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pujato, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Morein, Bror. Uppsala University. Department of Clinical Virology; Suecia
Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fuente
Research in Veterinary Science 96 (1) : 86-94 (February 2014)
Materia
Novilla
Ganado Bovino
Enfermedades de los Animales
Staphylococcus aureus
Vacuna
Respuesta Inmunológica
Coadyuvantes
Heifers
Cattle
Animal Diseases
Vaccines
Immune Response
Adjuvants
ISCOM Matrix
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2834
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2834
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network_name_str INTA Digital (INTA)
spelling Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvantCamussone, CeciliaVeaute, Carolina Melania IsabelPujato, NazarenaMorein, BrorMarcipar, Iván SergioCalvinho, Luis FernandoNovillaGanado BovinoEnfermedades de los AnimalesStaphylococcus aureusVacunaRespuesta InmunológicaCoadyuvantesHeifersCattleAnimal DiseasesVaccinesImmune ResponseAdjuvantsISCOM MatrixStaphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine.EEA RafaelaFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Pujato, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Morein, Bror. Uppsala University. Department of Clinical Virology; SueciaFil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina2018-07-20T12:09:39Z2018-07-20T12:09:39Z2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0034528813003214http://hdl.handle.net/20.500.12123/28340034-52881532-2661https://doi.org/10.1016/j.rvsc.2013.10.004Research in Veterinary Science 96 (1) : 86-94 (February 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:22Zoai:localhost:20.500.12123/2834instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:22.454INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
title Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
spellingShingle Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
Camussone, Cecilia
Novilla
Ganado Bovino
Enfermedades de los Animales
Staphylococcus aureus
Vacuna
Respuesta Inmunológica
Coadyuvantes
Heifers
Cattle
Animal Diseases
Vaccines
Immune Response
Adjuvants
ISCOM Matrix
title_short Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
title_full Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
title_fullStr Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
title_full_unstemmed Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
title_sort Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
dc.creator.none.fl_str_mv Camussone, Cecilia
Veaute, Carolina Melania Isabel
Pujato, Nazarena
Morein, Bror
Marcipar, Iván Sergio
Calvinho, Luis Fernando
author Camussone, Cecilia
author_facet Camussone, Cecilia
Veaute, Carolina Melania Isabel
Pujato, Nazarena
Morein, Bror
Marcipar, Iván Sergio
Calvinho, Luis Fernando
author_role author
author2 Veaute, Carolina Melania Isabel
Pujato, Nazarena
Morein, Bror
Marcipar, Iván Sergio
Calvinho, Luis Fernando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Novilla
Ganado Bovino
Enfermedades de los Animales
Staphylococcus aureus
Vacuna
Respuesta Inmunológica
Coadyuvantes
Heifers
Cattle
Animal Diseases
Vaccines
Immune Response
Adjuvants
ISCOM Matrix
topic Novilla
Ganado Bovino
Enfermedades de los Animales
Staphylococcus aureus
Vacuna
Respuesta Inmunológica
Coadyuvantes
Heifers
Cattle
Animal Diseases
Vaccines
Immune Response
Adjuvants
ISCOM Matrix
dc.description.none.fl_txt_mv Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine.
EEA Rafaela
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pujato, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Morein, Bror. Uppsala University. Department of Clinical Virology; Suecia
Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
description Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
2018-07-20T12:09:39Z
2018-07-20T12:09:39Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0034528813003214
http://hdl.handle.net/20.500.12123/2834
0034-5288
1532-2661
https://doi.org/10.1016/j.rvsc.2013.10.004
url https://www.sciencedirect.com/science/article/pii/S0034528813003214
http://hdl.handle.net/20.500.12123/2834
https://doi.org/10.1016/j.rvsc.2013.10.004
identifier_str_mv 0034-5288
1532-2661
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Research in Veterinary Science 96 (1) : 86-94 (February 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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