Development and assessment of a new cage-like particle adjuvant

Autores
Bertona, Daiana; Pujato, Nazarena; Bontempi, Iván; González, Verónica Doris Guadalupe; Cabrera, Gabriel Gustavo; Gugliotta, Luis Marcelino; Hozbor, Daniela Flavia; Nicastro, Alcides; Calvinho, Luis Fernando; Marcipar, Iván Sergio
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: To obtain and assess stable cage-like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage-like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX? performance. Particle size and adjuvant performance were conserved during the 6-month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans-sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.
Fil: Bertona, Daiana. Universidad Nacional del Litoral; Argentina
Fil: Pujato, Nazarena. Universidad Nacional del Litoral; Argentina
Fil: Bontempi, Iván. Universidad Nacional del Litoral; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nicastro, Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral; Argentina
Materia
Adjuvant
Cage-Like Particle
Cellular Response
Humoral Response
Low Charge
Trypanosoma Cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43671
Acceder
id CONICETDig_9babfdfe8ec48a9a243f913fee91500a
oai_identifier_str oai:ri.conicet.gov.ar:11336/43671
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and assessment of a new cage-like particle adjuvantBertona, DaianaPujato, NazarenaBontempi, IvánGonzález, Verónica Doris GuadalupeCabrera, Gabriel GustavoGugliotta, Luis MarcelinoHozbor, Daniela FlaviaNicastro, AlcidesCalvinho, Luis FernandoMarcipar, Iván SergioAdjuvantCage-Like ParticleCellular ResponseHumoral ResponseLow ChargeTrypanosoma Cruzihttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Objectives: To obtain and assess stable cage-like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage-like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX? performance. Particle size and adjuvant performance were conserved during the 6-month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans-sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.Fil: Bertona, Daiana. Universidad Nacional del Litoral; ArgentinaFil: Pujato, Nazarena. Universidad Nacional del Litoral; ArgentinaFil: Bontempi, Iván. Universidad Nacional del Litoral; ArgentinaFil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Cabrera, Gabriel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nicastro, Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Marcipar, Iván Sergio. Universidad Nacional del Litoral; ArgentinaPharmaceutical Press-Royal Pharmaceutical Society Great Britian2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43671Bertona, Daiana; Pujato, Nazarena; Bontempi, Iván; González, Verónica Doris Guadalupe; Cabrera, Gabriel Gustavo; et al.; Development and assessment of a new cage-like particle adjuvant; Pharmaceutical Press-Royal Pharmaceutical Society Great Britian; Journal of Pharmacy and Pharmacology; 69; 10; 10-2017; 1293-13030022-3573CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/jphp.12768info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:39:32Zoai:ri.conicet.gov.ar:11336/43671instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:39:33.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and assessment of a new cage-like particle adjuvant
title Development and assessment of a new cage-like particle adjuvant
spellingShingle Development and assessment of a new cage-like particle adjuvant
Bertona, Daiana
Adjuvant
Cage-Like Particle
Cellular Response
Humoral Response
Low Charge
Trypanosoma Cruzi
title_short Development and assessment of a new cage-like particle adjuvant
title_full Development and assessment of a new cage-like particle adjuvant
title_fullStr Development and assessment of a new cage-like particle adjuvant
title_full_unstemmed Development and assessment of a new cage-like particle adjuvant
title_sort Development and assessment of a new cage-like particle adjuvant
dc.creator.none.fl_str_mv Bertona, Daiana
Pujato, Nazarena
Bontempi, Iván
González, Verónica Doris Guadalupe
Cabrera, Gabriel Gustavo
Gugliotta, Luis Marcelino
Hozbor, Daniela Flavia
Nicastro, Alcides
Calvinho, Luis Fernando
Marcipar, Iván Sergio
author Bertona, Daiana
author_facet Bertona, Daiana
Pujato, Nazarena
Bontempi, Iván
González, Verónica Doris Guadalupe
Cabrera, Gabriel Gustavo
Gugliotta, Luis Marcelino
Hozbor, Daniela Flavia
Nicastro, Alcides
Calvinho, Luis Fernando
Marcipar, Iván Sergio
author_role author
author2 Pujato, Nazarena
Bontempi, Iván
González, Verónica Doris Guadalupe
Cabrera, Gabriel Gustavo
Gugliotta, Luis Marcelino
Hozbor, Daniela Flavia
Nicastro, Alcides
Calvinho, Luis Fernando
Marcipar, Iván Sergio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adjuvant
Cage-Like Particle
Cellular Response
Humoral Response
Low Charge
Trypanosoma Cruzi
topic Adjuvant
Cage-Like Particle
Cellular Response
Humoral Response
Low Charge
Trypanosoma Cruzi
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Objectives: To obtain and assess stable cage-like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage-like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX? performance. Particle size and adjuvant performance were conserved during the 6-month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans-sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.
Fil: Bertona, Daiana. Universidad Nacional del Litoral; Argentina
Fil: Pujato, Nazarena. Universidad Nacional del Litoral; Argentina
Fil: Bontempi, Iván. Universidad Nacional del Litoral; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nicastro, Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral; Argentina
description Objectives: To obtain and assess stable cage-like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage-like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX? performance. Particle size and adjuvant performance were conserved during the 6-month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans-sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43671
Bertona, Daiana; Pujato, Nazarena; Bontempi, Iván; González, Verónica Doris Guadalupe; Cabrera, Gabriel Gustavo; et al.; Development and assessment of a new cage-like particle adjuvant; Pharmaceutical Press-Royal Pharmaceutical Society Great Britian; Journal of Pharmacy and Pharmacology; 69; 10; 10-2017; 1293-1303
0022-3573
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43671
identifier_str_mv Bertona, Daiana; Pujato, Nazarena; Bontempi, Iván; González, Verónica Doris Guadalupe; Cabrera, Gabriel Gustavo; et al.; Development and assessment of a new cage-like particle adjuvant; Pharmaceutical Press-Royal Pharmaceutical Society Great Britian; Journal of Pharmacy and Pharmacology; 69; 10; 10-2017; 1293-1303
0022-3573
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/jphp.12768
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pharmaceutical Press-Royal Pharmaceutical Society Great Britian
publisher.none.fl_str_mv Pharmaceutical Press-Royal Pharmaceutical Society Great Britian
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597449265905664
score 12.976206

Publicaciones similares