Analysis of irradiated Argentinean fetal bovine serum for adventitious agents

Autores
Pecora, Andrea; Perez Lopez, Jorgelina; Jordan, Maximiliano Jesus; Franco, Lautaro Nahuel; Politzki, Romina Paula; Ruiz, Vanesa; Alvarez, Irene
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them.
Instituto de Virología
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Lopez, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Jordan, Maximiliano Jesus. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Franco, Lautaro Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fuente
Journal of Veterinary Diagnostic Investigation 32 (6) : 892–897 (Noviembre 2020)
Materia
Argentina
Contaminación
Mycoplasma
Virus
Contamination
Viruses
Fetal Bovine Serum
Adventitious Agents
Pestiviruses
Suero Fetal Bovino
Agentes Adventicios
Pestivirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8671
Acceder
id INTADig_2d8306725167e185c69a9a473d7f54bf
oai_identifier_str oai:localhost:20.500.12123/8671
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Analysis of irradiated Argentinean fetal bovine serum for adventitious agentsPecora, AndreaPerez Lopez, JorgelinaJordan, Maximiliano JesusFranco, Lautaro NahuelPolitzki, Romina PaulaRuiz, VanesaAlvarez, IreneArgentinaContaminaciónMycoplasmaVirusContaminationVirusesFetal Bovine SerumAdventitious AgentsPestivirusesSuero Fetal BovinoAgentes AdventiciosPestivirusFetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them.Instituto de VirologíaFil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Lopez, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Jordan, Maximiliano Jesus. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Franco, Lautaro Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaSage Publications2021-02-17T16:31:29Z2021-02-17T16:31:29Z2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8671https://journals.sagepub.com/doi/abs/10.1177/10406387209515561943-4936https://doi.org/10.1177/1040638720951556Journal of Veterinary Diagnostic Investigation 32 (6) : 892–897 (Noviembre 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repo/semantics/restrictedAccess2025-11-27T08:38:42Zoai:localhost:20.500.12123/8671instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-27 08:38:43.285INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
title Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
spellingShingle Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
Pecora, Andrea
Argentina
Contaminación
Mycoplasma
Virus
Contamination
Viruses
Fetal Bovine Serum
Adventitious Agents
Pestiviruses
Suero Fetal Bovino
Agentes Adventicios
Pestivirus
title_short Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
title_full Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
title_fullStr Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
title_full_unstemmed Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
title_sort Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
dc.creator.none.fl_str_mv Pecora, Andrea
Perez Lopez, Jorgelina
Jordan, Maximiliano Jesus
Franco, Lautaro Nahuel
Politzki, Romina Paula
Ruiz, Vanesa
Alvarez, Irene
author Pecora, Andrea
author_facet Pecora, Andrea
Perez Lopez, Jorgelina
Jordan, Maximiliano Jesus
Franco, Lautaro Nahuel
Politzki, Romina Paula
Ruiz, Vanesa
Alvarez, Irene
author_role author
author2 Perez Lopez, Jorgelina
Jordan, Maximiliano Jesus
Franco, Lautaro Nahuel
Politzki, Romina Paula
Ruiz, Vanesa
Alvarez, Irene
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Argentina
Contaminación
Mycoplasma
Virus
Contamination
Viruses
Fetal Bovine Serum
Adventitious Agents
Pestiviruses
Suero Fetal Bovino
Agentes Adventicios
Pestivirus
topic Argentina
Contaminación
Mycoplasma
Virus
Contamination
Viruses
Fetal Bovine Serum
Adventitious Agents
Pestiviruses
Suero Fetal Bovino
Agentes Adventicios
Pestivirus
dc.description.none.fl_txt_mv Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them.
Instituto de Virología
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Lopez, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Jordan, Maximiliano Jesus. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Franco, Lautaro Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
description Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
2021-02-17T16:31:29Z
2021-02-17T16:31:29Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8671
https://journals.sagepub.com/doi/abs/10.1177/1040638720951556
1943-4936
https://doi.org/10.1177/1040638720951556
url http://hdl.handle.net/20.500.12123/8671
https://journals.sagepub.com/doi/abs/10.1177/1040638720951556
https://doi.org/10.1177/1040638720951556
identifier_str_mv 1943-4936
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sage Publications
publisher.none.fl_str_mv Sage Publications
dc.source.none.fl_str_mv Journal of Veterinary Diagnostic Investigation 32 (6) : 892–897 (Noviembre 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1849949283964420096
score 13.011256

Publicaciones similares