Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
- Autores
- De Breuil, Soledad; Dottori, Carolina Andrea; Bejerman, Nicolas Esteban; Nome Docampo, Claudia; Giolitti, Fabian; Lenardon, Sergio Luis
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species.
Instituto de Patología Vegetal
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina - Fuente
- Journal of Plant Pathology (October 2020)
- Materia
-
Arachis Hypogaea
Argentina
Virus de las Plantas
Plant Viruses
Outbreak
Orthotospovirus
Maní - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8366
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Orthotospovirus disease epidemic: molecular characterization and incidence in peanut cropsDe Breuil, SoledadDottori, Carolina AndreaBejerman, Nicolas EstebanNome Docampo, ClaudiaGiolitti, FabianLenardon, Sergio LuisArachis HypogaeaArgentinaVirus de las PlantasPlant VirusesOutbreakOrthotospovirusManíArgentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species.Instituto de Patología VegetalFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaSpringer2020-12-02T14:20:28Z2020-12-02T14:20:28Z2020-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8366https://link.springer.com/article/10.1007/s42161-020-00686-01125-46532239-7264 (online)https://doi.org/10.1007/s42161-020-00686-0Journal of Plant Pathology (October 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:05Zoai:localhost:20.500.12123/8366instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:05.914INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
title |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
spellingShingle |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops De Breuil, Soledad Arachis Hypogaea Argentina Virus de las Plantas Plant Viruses Outbreak Orthotospovirus Maní |
title_short |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
title_full |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
title_fullStr |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
title_full_unstemmed |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
title_sort |
Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
dc.creator.none.fl_str_mv |
De Breuil, Soledad Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis |
author |
De Breuil, Soledad |
author_facet |
De Breuil, Soledad Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis |
author_role |
author |
author2 |
Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Arachis Hypogaea Argentina Virus de las Plantas Plant Viruses Outbreak Orthotospovirus Maní |
topic |
Arachis Hypogaea Argentina Virus de las Plantas Plant Viruses Outbreak Orthotospovirus Maní |
dc.description.none.fl_txt_mv |
Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species. Instituto de Patología Vegetal Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina |
description |
Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-02T14:20:28Z 2020-12-02T14:20:28Z 2020-10-13 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/8366 https://link.springer.com/article/10.1007/s42161-020-00686-0 1125-4653 2239-7264 (online) https://doi.org/10.1007/s42161-020-00686-0 |
url |
http://hdl.handle.net/20.500.12123/8366 https://link.springer.com/article/10.1007/s42161-020-00686-0 https://doi.org/10.1007/s42161-020-00686-0 |
identifier_str_mv |
1125-4653 2239-7264 (online) |
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eng |
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eng |
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info:eu-repo/semantics/restrictedAccess |
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restrictedAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
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Springer |
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Journal of Plant Pathology (October 2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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