Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops

Autores
De Breuil, Soledad; Dottori, Carolina Andrea; Bejerman, Nicolas Esteban; Nome Docampo, Claudia; Giolitti, Fabian; Lenardon, Sergio Luis
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species.
Instituto de Patología Vegetal
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fuente
Journal of Plant Pathology (October 2020)
Materia
Arachis Hypogaea
Argentina
Virus de las Plantas
Plant Viruses
Outbreak
Orthotospovirus
Maní
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Orthotospovirus disease epidemic: molecular characterization and incidence in peanut cropsDe Breuil, SoledadDottori, Carolina AndreaBejerman, Nicolas EstebanNome Docampo, ClaudiaGiolitti, FabianLenardon, Sergio LuisArachis HypogaeaArgentinaVirus de las PlantasPlant VirusesOutbreakOrthotospovirusManíArgentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species.Instituto de Patología VegetalFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaSpringer2020-12-02T14:20:28Z2020-12-02T14:20:28Z2020-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8366https://link.springer.com/article/10.1007/s42161-020-00686-01125-46532239-7264 (online)https://doi.org/10.1007/s42161-020-00686-0Journal of Plant Pathology (October 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:58Zoai:localhost:20.500.12123/8366instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:58.263INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
title Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
spellingShingle Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
De Breuil, Soledad
Arachis Hypogaea
Argentina
Virus de las Plantas
Plant Viruses
Outbreak
Orthotospovirus
Maní
title_short Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
title_full Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
title_fullStr Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
title_full_unstemmed Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
title_sort Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
dc.creator.none.fl_str_mv De Breuil, Soledad
Dottori, Carolina Andrea
Bejerman, Nicolas Esteban
Nome Docampo, Claudia
Giolitti, Fabian
Lenardon, Sergio Luis
author De Breuil, Soledad
author_facet De Breuil, Soledad
Dottori, Carolina Andrea
Bejerman, Nicolas Esteban
Nome Docampo, Claudia
Giolitti, Fabian
Lenardon, Sergio Luis
author_role author
author2 Dottori, Carolina Andrea
Bejerman, Nicolas Esteban
Nome Docampo, Claudia
Giolitti, Fabian
Lenardon, Sergio Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Arachis Hypogaea
Argentina
Virus de las Plantas
Plant Viruses
Outbreak
Orthotospovirus
Maní
topic Arachis Hypogaea
Argentina
Virus de las Plantas
Plant Viruses
Outbreak
Orthotospovirus
Maní
dc.description.none.fl_txt_mv Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species.
Instituto de Patología Vegetal
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
description Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-02T14:20:28Z
2020-12-02T14:20:28Z
2020-10-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8366
https://link.springer.com/article/10.1007/s42161-020-00686-0
1125-4653
2239-7264 (online)
https://doi.org/10.1007/s42161-020-00686-0
url http://hdl.handle.net/20.500.12123/8366
https://link.springer.com/article/10.1007/s42161-020-00686-0
https://doi.org/10.1007/s42161-020-00686-0
identifier_str_mv 1125-4653
2239-7264 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Plant Pathology (October 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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