A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle

Autores
Muntaabski, Irina; Russo, Romina Maria; Liendo, María Clara; Landi, Lucas; Lanzavecchia, Silvia Beatriz; Scannapieco, Alejandra Carla
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments.
Instituto de Investigación Genética
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; (CONICET) Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina
Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
Acarologia 63 (2) : 383-389 (Marzo 2023)
Materia
Varroa destructor
Apis mellifera
Relaciones Huésped Parásito
Ácaros
Host Parasite Relations
Mites
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15173
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15173
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycleMuntaabski, IrinaRusso, Romina MariaLiendo, María ClaraLandi, LucasLanzavecchia, Silvia BeatrizScannapieco, Alejandra CarlaVarroa destructorApis melliferaRelaciones Huésped ParásitoÁcarosHost Parasite RelationsMitesVarroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments.Instituto de Investigación GenéticaFil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; (CONICET) ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); ArgentinaFil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaLes Amis d'Acarologia2023-09-12T10:06:22Z2023-09-12T10:06:22Z2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15173https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=45912107-7207https://doi.org/10.24349/eyu1-ewegAcarologia 63 (2) : 383-389 (Marzo 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E1-I017-001, DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVOinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:56Zoai:localhost:20.500.12123/15173instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:56.418INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
title A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
spellingShingle A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
Muntaabski, Irina
Varroa destructor
Apis mellifera
Relaciones Huésped Parásito
Ácaros
Host Parasite Relations
Mites
title_short A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
title_full A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
title_fullStr A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
title_full_unstemmed A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
title_sort A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
dc.creator.none.fl_str_mv Muntaabski, Irina
Russo, Romina Maria
Liendo, María Clara
Landi, Lucas
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author Muntaabski, Irina
author_facet Muntaabski, Irina
Russo, Romina Maria
Liendo, María Clara
Landi, Lucas
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author_role author
author2 Russo, Romina Maria
Liendo, María Clara
Landi, Lucas
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Varroa destructor
Apis mellifera
Relaciones Huésped Parásito
Ácaros
Host Parasite Relations
Mites
topic Varroa destructor
Apis mellifera
Relaciones Huésped Parásito
Ácaros
Host Parasite Relations
Mites
dc.description.none.fl_txt_mv Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments.
Instituto de Investigación Genética
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; (CONICET) Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina
Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-12T10:06:22Z
2023-09-12T10:06:22Z
2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15173
https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4591
2107-7207
https://doi.org/10.24349/eyu1-eweg
url http://hdl.handle.net/20.500.12123/15173
https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4591
https://doi.org/10.24349/eyu1-eweg
identifier_str_mv 2107-7207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001, DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVO
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Les Amis d'Acarologia
publisher.none.fl_str_mv Les Amis d'Acarologia
dc.source.none.fl_str_mv Acarologia 63 (2) : 383-389 (Marzo 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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