Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus

Autores
Yanniccari, Marcos; Palma Bautista, Candelario; Vázquez García, José Guadalupe; Gigon, Ramon; Mallory-Smith, Carol Ann; de Prado, Rafael
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Background: Glyphosate-resistant Salsola tragus accessions have been identified in the USA and Argentina; however, the mechanisms of glyphosate resistance have not been elucidated. The goal of this study was to determine the mechanism/s of glyphosate resistance involved in two S. tragus populations (R1 and R2) from Argentina. Results: Both glyphosate-resistant populations had a 6-fold lower sensitivity to glyphosate than the S population (i.e. resistance index). No evidence of differential absorption, translocation or metabolism of glyphosate was found in the R1 and R2 populations compared to a susceptible population (S). No EPSPS mutations were detected, but S. tragus R1 and R2 plants had approximately 14-fold higher EPSPS gene relative copy number compared to the S counterpart. In R1 and R2, EPSPS duplication entailed a greater constitutive EPSPS transcript abundance by about 7-fold and a basal EPSPS activity approximately 3-fold higher than the S population. Conclusion: The current study reports EPSPS gene duplication for the first time as a mechanism of glyphosate resistance in S. tragus populations. The increase of glyphosate dose needed to kill R1 and R2 plants was linked to the EPSPS transcript abundance and level of EPSPS activity. This evidence supports the convergent evolution of the overexpression EPSPS gene in several Chenopodiaceae/Amaranthaceae species adapted to drought environments and the role of gene duplication as an adaptive advantage for plants to withstand stress.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fil: Mallory-Smith, Carol Ann. Oregon State University. Department of Crop and Soil Science; Estados Unidos
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fuente
Pest Management Science (First published: 03 November 2022)
Materia
Salsola
Glifosato
Resistencia a los Herbicidas
Escarda
Malezas
Glyphosate
Resistance to Herbicides
Weed Control
Weeds
Salsola tragus
Control de Malezas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13420
Acceder
id INTADig_2a5b1c3c0c51023dd3ee0050b7bb9f37
oai_identifier_str oai:localhost:20.500.12123/13420
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragusYanniccari, MarcosPalma Bautista, CandelarioVázquez García, José GuadalupeGigon, RamonMallory-Smith, Carol Annde Prado, RafaelSalsolaGlifosatoResistencia a los HerbicidasEscardaMalezasGlyphosateResistance to HerbicidesWeed ControlWeedsSalsola tragusControl de MalezasBackground: Glyphosate-resistant Salsola tragus accessions have been identified in the USA and Argentina; however, the mechanisms of glyphosate resistance have not been elucidated. The goal of this study was to determine the mechanism/s of glyphosate resistance involved in two S. tragus populations (R1 and R2) from Argentina. Results: Both glyphosate-resistant populations had a 6-fold lower sensitivity to glyphosate than the S population (i.e. resistance index). No evidence of differential absorption, translocation or metabolism of glyphosate was found in the R1 and R2 populations compared to a susceptible population (S). No EPSPS mutations were detected, but S. tragus R1 and R2 plants had approximately 14-fold higher EPSPS gene relative copy number compared to the S counterpart. In R1 and R2, EPSPS duplication entailed a greater constitutive EPSPS transcript abundance by about 7-fold and a basal EPSPS activity approximately 3-fold higher than the S population. Conclusion: The current study reports EPSPS gene duplication for the first time as a mechanism of glyphosate resistance in S. tragus populations. The increase of glyphosate dose needed to kill R1 and R2 plants was linked to the EPSPS transcript abundance and level of EPSPS activity. This evidence supports the convergent evolution of the overexpression EPSPS gene in several Chenopodiaceae/Amaranthaceae species adapted to drought environments and the role of gene duplication as an adaptive advantage for plants to withstand stress.EEA BarrowFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Gigón, Ramón. Consultor privado en Control de Malezas; ArgentinaFil: Mallory-Smith, Carol Ann. Oregon State University. Department of Crop and Soil Science; Estados UnidosFil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaWileyinfo:eu-repo/date/embargoEnd/2023-11-152022-11-15T12:46:40Z2022-11-15T12:46:40Z2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13420https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.72721526-498X1526-4998https://doi.org/10.1002/ps.7272Pest Management Science (First published: 03 November 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E4-I086-001/2019-PE-E4-I086-001/AR./Monitoreo, caracterización y manejo de plagas resistentes a fitosanitarios y organismos geneticamente modificadosinfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:59Zoai:localhost:20.500.12123/13420instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:00.217INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
title Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
spellingShingle Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
Yanniccari, Marcos
Salsola
Glifosato
Resistencia a los Herbicidas
Escarda
Malezas
Glyphosate
Resistance to Herbicides
Weed Control
Weeds
Salsola tragus
Control de Malezas
title_short Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
title_full Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
title_fullStr Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
title_full_unstemmed Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
title_sort Constitutive overexpression of EPSPS by gene duplication is involved in glyphosate resistance in Salsola tragus
dc.creator.none.fl_str_mv Yanniccari, Marcos
Palma Bautista, Candelario
Vázquez García, José Guadalupe
Gigon, Ramon
Mallory-Smith, Carol Ann
de Prado, Rafael
author Yanniccari, Marcos
author_facet Yanniccari, Marcos
Palma Bautista, Candelario
Vázquez García, José Guadalupe
Gigon, Ramon
Mallory-Smith, Carol Ann
de Prado, Rafael
author_role author
author2 Palma Bautista, Candelario
Vázquez García, José Guadalupe
Gigon, Ramon
Mallory-Smith, Carol Ann
de Prado, Rafael
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Salsola
Glifosato
Resistencia a los Herbicidas
Escarda
Malezas
Glyphosate
Resistance to Herbicides
Weed Control
Weeds
Salsola tragus
Control de Malezas
topic Salsola
Glifosato
Resistencia a los Herbicidas
Escarda
Malezas
Glyphosate
Resistance to Herbicides
Weed Control
Weeds
Salsola tragus
Control de Malezas
dc.description.none.fl_txt_mv Background: Glyphosate-resistant Salsola tragus accessions have been identified in the USA and Argentina; however, the mechanisms of glyphosate resistance have not been elucidated. The goal of this study was to determine the mechanism/s of glyphosate resistance involved in two S. tragus populations (R1 and R2) from Argentina. Results: Both glyphosate-resistant populations had a 6-fold lower sensitivity to glyphosate than the S population (i.e. resistance index). No evidence of differential absorption, translocation or metabolism of glyphosate was found in the R1 and R2 populations compared to a susceptible population (S). No EPSPS mutations were detected, but S. tragus R1 and R2 plants had approximately 14-fold higher EPSPS gene relative copy number compared to the S counterpart. In R1 and R2, EPSPS duplication entailed a greater constitutive EPSPS transcript abundance by about 7-fold and a basal EPSPS activity approximately 3-fold higher than the S population. Conclusion: The current study reports EPSPS gene duplication for the first time as a mechanism of glyphosate resistance in S. tragus populations. The increase of glyphosate dose needed to kill R1 and R2 plants was linked to the EPSPS transcript abundance and level of EPSPS activity. This evidence supports the convergent evolution of the overexpression EPSPS gene in several Chenopodiaceae/Amaranthaceae species adapted to drought environments and the role of gene duplication as an adaptive advantage for plants to withstand stress.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fil: Mallory-Smith, Carol Ann. Oregon State University. Department of Crop and Soil Science; Estados Unidos
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
description Background: Glyphosate-resistant Salsola tragus accessions have been identified in the USA and Argentina; however, the mechanisms of glyphosate resistance have not been elucidated. The goal of this study was to determine the mechanism/s of glyphosate resistance involved in two S. tragus populations (R1 and R2) from Argentina. Results: Both glyphosate-resistant populations had a 6-fold lower sensitivity to glyphosate than the S population (i.e. resistance index). No evidence of differential absorption, translocation or metabolism of glyphosate was found in the R1 and R2 populations compared to a susceptible population (S). No EPSPS mutations were detected, but S. tragus R1 and R2 plants had approximately 14-fold higher EPSPS gene relative copy number compared to the S counterpart. In R1 and R2, EPSPS duplication entailed a greater constitutive EPSPS transcript abundance by about 7-fold and a basal EPSPS activity approximately 3-fold higher than the S population. Conclusion: The current study reports EPSPS gene duplication for the first time as a mechanism of glyphosate resistance in S. tragus populations. The increase of glyphosate dose needed to kill R1 and R2 plants was linked to the EPSPS transcript abundance and level of EPSPS activity. This evidence supports the convergent evolution of the overexpression EPSPS gene in several Chenopodiaceae/Amaranthaceae species adapted to drought environments and the role of gene duplication as an adaptive advantage for plants to withstand stress.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-15T12:46:40Z
2022-11-15T12:46:40Z
2022-11
info:eu-repo/date/embargoEnd/2023-11-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13420
https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.7272
1526-498X
1526-4998
https://doi.org/10.1002/ps.7272
url http://hdl.handle.net/20.500.12123/13420
https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.7272
https://doi.org/10.1002/ps.7272
identifier_str_mv 1526-498X
1526-4998
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E4-I086-001/2019-PE-E4-I086-001/AR./Monitoreo, caracterización y manejo de plagas resistentes a fitosanitarios y organismos geneticamente modificados
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Pest Management Science (First published: 03 November 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143554208923648
score 12.711113

Publicaciones similares