A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis

Autores
Yanniccari, Marcos; Vázquez García, José Guadalupe; Gigon, Ramon; Palma Bautista, Candelario; Vila-Aiub, Martín Miguel; de Prado, Rafael
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
BACKGROUND: Digitaria sanguinalis has been identified as a species at high risk of evolving herbicide resistance, but thus far, there are no records of resistance to glyphosate. This species is one of the most common weeds of summer crops in extensive cropping areas in Argentina. This weed shows an extended period of seedling emergence with several overlapping cohorts during spring and summer, and it is commonly controlled with glyphosate. However, a D. sanguinalis population was implicated as a putative glyphosate-resistant biotype based on poor control at recommended glyphosate doses. RESULTS: The field-collected D. sanguinalis population (Dgs R) from the Rolling Pampas has evolved glyphosate resistance. Differences in plant survival and shikimate levels after field recommended and higher glyphosate doses were evident between Dgs R and the known susceptible (Dgs S) population, and the resistance index was 5.1. No evidence of differential glyphosate absorption, translocation, metabolism, or basal EPSPS activity was found between Dgs S and Dgs R populations; however, a novel EPSPS Pro-106-His point substitution is likely the primary glyphosate resistance endowing mechanism. EPSPS in vitro enzymatic activity demonstrated that an 80-fold higher concentration of glyphosate is required in Dgs R to achieve similar EPSPS activity inhibition as in the Dgs S population. CONCLUSION: This study reports the first global case of glyphosate resistance in D. sanguinalis. This yet novel transversion at the second position of the EPSPS 106 codon demonstrates the intensity of glyphosate pressure in selecting unexpected glyphosate resistance alleles if they retain EPSPS functionality.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología y Genética Vegetal; Argentina.
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Vila Aiub, Martin Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fuente
Pest Management Science (First published: 22 April 2022)
Materia
Digitaria sanguinalis
Glifosato
Resistencia a los Herbicidas
Malezas
Escarda
Genética
Glyphosate
Resistance to Herbicides
Weeds
Weed Control
Genetics
Control de Malezas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11736
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11736
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalisYanniccari, MarcosVázquez García, José GuadalupeGigon, RamonPalma Bautista, CandelarioVila-Aiub, Martín Miguelde Prado, RafaelDigitaria sanguinalisGlifosatoResistencia a los HerbicidasMalezasEscardaGenéticaGlyphosateResistance to HerbicidesWeedsWeed ControlGeneticsControl de MalezasBACKGROUND: Digitaria sanguinalis has been identified as a species at high risk of evolving herbicide resistance, but thus far, there are no records of resistance to glyphosate. This species is one of the most common weeds of summer crops in extensive cropping areas in Argentina. This weed shows an extended period of seedling emergence with several overlapping cohorts during spring and summer, and it is commonly controlled with glyphosate. However, a D. sanguinalis population was implicated as a putative glyphosate-resistant biotype based on poor control at recommended glyphosate doses. RESULTS: The field-collected D. sanguinalis population (Dgs R) from the Rolling Pampas has evolved glyphosate resistance. Differences in plant survival and shikimate levels after field recommended and higher glyphosate doses were evident between Dgs R and the known susceptible (Dgs S) population, and the resistance index was 5.1. No evidence of differential glyphosate absorption, translocation, metabolism, or basal EPSPS activity was found between Dgs S and Dgs R populations; however, a novel EPSPS Pro-106-His point substitution is likely the primary glyphosate resistance endowing mechanism. EPSPS in vitro enzymatic activity demonstrated that an 80-fold higher concentration of glyphosate is required in Dgs R to achieve similar EPSPS activity inhibition as in the Dgs S population. CONCLUSION: This study reports the first global case of glyphosate resistance in D. sanguinalis. This yet novel transversion at the second position of the EPSPS 106 codon demonstrates the intensity of glyphosate pressure in selecting unexpected glyphosate resistance alleles if they retain EPSPS functionality.EEA BarrowFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología y Genética Vegetal; Argentina.Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Gigón, Ramón. Consultor privado en Control de Malezas; ArgentinaFil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.Fil: Vila Aiub, Martin Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaWileyinfo:eu-repo/date/embargoEnd/2023-04-262022-04-26T12:05:53Z2022-04-26T12:05:53Z2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11736https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.69401526-498X1526-4998https://doi.org/10.1002/ps.6940Pest Management Science (First published: 22 April 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:08:34Zoai:localhost:20.500.12123/11736instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:08:35.128INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
title A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
spellingShingle A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
Yanniccari, Marcos
Digitaria sanguinalis
Glifosato
Resistencia a los Herbicidas
Malezas
Escarda
Genética
Glyphosate
Resistance to Herbicides
Weeds
Weed Control
Genetics
Control de Malezas
title_short A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
title_full A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
title_fullStr A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
title_full_unstemmed A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
title_sort A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis
dc.creator.none.fl_str_mv Yanniccari, Marcos
Vázquez García, José Guadalupe
Gigon, Ramon
Palma Bautista, Candelario
Vila-Aiub, Martín Miguel
de Prado, Rafael
author Yanniccari, Marcos
author_facet Yanniccari, Marcos
Vázquez García, José Guadalupe
Gigon, Ramon
Palma Bautista, Candelario
Vila-Aiub, Martín Miguel
de Prado, Rafael
author_role author
author2 Vázquez García, José Guadalupe
Gigon, Ramon
Palma Bautista, Candelario
Vila-Aiub, Martín Miguel
de Prado, Rafael
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Digitaria sanguinalis
Glifosato
Resistencia a los Herbicidas
Malezas
Escarda
Genética
Glyphosate
Resistance to Herbicides
Weeds
Weed Control
Genetics
Control de Malezas
topic Digitaria sanguinalis
Glifosato
Resistencia a los Herbicidas
Malezas
Escarda
Genética
Glyphosate
Resistance to Herbicides
Weeds
Weed Control
Genetics
Control de Malezas
dc.description.none.fl_txt_mv BACKGROUND: Digitaria sanguinalis has been identified as a species at high risk of evolving herbicide resistance, but thus far, there are no records of resistance to glyphosate. This species is one of the most common weeds of summer crops in extensive cropping areas in Argentina. This weed shows an extended period of seedling emergence with several overlapping cohorts during spring and summer, and it is commonly controlled with glyphosate. However, a D. sanguinalis population was implicated as a putative glyphosate-resistant biotype based on poor control at recommended glyphosate doses. RESULTS: The field-collected D. sanguinalis population (Dgs R) from the Rolling Pampas has evolved glyphosate resistance. Differences in plant survival and shikimate levels after field recommended and higher glyphosate doses were evident between Dgs R and the known susceptible (Dgs S) population, and the resistance index was 5.1. No evidence of differential glyphosate absorption, translocation, metabolism, or basal EPSPS activity was found between Dgs S and Dgs R populations; however, a novel EPSPS Pro-106-His point substitution is likely the primary glyphosate resistance endowing mechanism. EPSPS in vitro enzymatic activity demonstrated that an 80-fold higher concentration of glyphosate is required in Dgs R to achieve similar EPSPS activity inhibition as in the Dgs S population. CONCLUSION: This study reports the first global case of glyphosate resistance in D. sanguinalis. This yet novel transversion at the second position of the EPSPS 106 codon demonstrates the intensity of glyphosate pressure in selecting unexpected glyphosate resistance alleles if they retain EPSPS functionality.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología y Genética Vegetal; Argentina.
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Vila Aiub, Martin Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
description BACKGROUND: Digitaria sanguinalis has been identified as a species at high risk of evolving herbicide resistance, but thus far, there are no records of resistance to glyphosate. This species is one of the most common weeds of summer crops in extensive cropping areas in Argentina. This weed shows an extended period of seedling emergence with several overlapping cohorts during spring and summer, and it is commonly controlled with glyphosate. However, a D. sanguinalis population was implicated as a putative glyphosate-resistant biotype based on poor control at recommended glyphosate doses. RESULTS: The field-collected D. sanguinalis population (Dgs R) from the Rolling Pampas has evolved glyphosate resistance. Differences in plant survival and shikimate levels after field recommended and higher glyphosate doses were evident between Dgs R and the known susceptible (Dgs S) population, and the resistance index was 5.1. No evidence of differential glyphosate absorption, translocation, metabolism, or basal EPSPS activity was found between Dgs S and Dgs R populations; however, a novel EPSPS Pro-106-His point substitution is likely the primary glyphosate resistance endowing mechanism. EPSPS in vitro enzymatic activity demonstrated that an 80-fold higher concentration of glyphosate is required in Dgs R to achieve similar EPSPS activity inhibition as in the Dgs S population. CONCLUSION: This study reports the first global case of glyphosate resistance in D. sanguinalis. This yet novel transversion at the second position of the EPSPS 106 codon demonstrates the intensity of glyphosate pressure in selecting unexpected glyphosate resistance alleles if they retain EPSPS functionality.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-26T12:05:53Z
2022-04-26T12:05:53Z
2022-04
info:eu-repo/date/embargoEnd/2023-04-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11736
https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.6940
1526-498X
1526-4998
https://doi.org/10.1002/ps.6940
url http://hdl.handle.net/20.500.12123/11736
https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.6940
https://doi.org/10.1002/ps.6940
identifier_str_mv 1526-498X
1526-4998
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Pest Management Science (First published: 22 April 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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