Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture

Autores
Yanniccari, Marcos; Gigon, Ramon
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart. RESULTS: Plants of the EPSPS overexpression biotype were most sensitive to glyphosate when it was combined with clethodim, and the dose required to halve the maximal response (I50) calculated for glyphosate was reduced to a third. However, the ACCase inhibitor seems to be uniquely responsible for weed control in the P106S biotype. In response to clethodim, EPSPS expression was inhibited in the glyphosate-resistant EPSPS overexpression biotype to a level like or lower than that of susceptible plants. The glyphosate plus clethodim mixture caused a 1.7-fold increase in shikimate concentration in the leaves of susceptible and EPSPS overexpression biotypes compared to glyphosate alone. CONCLUSION: The effects of glyphosate plus clethodim impact in different senses according to the mechanism of resistance involved, where the convergent action of both herbicides controlled the susceptible and EPSPS overexpression biotypes. In contrast, the P106S biotype died because of the clethodim component in the mixture. Mixing herbicides should not be considered a complete solution to herbicide resistance management, and its potential seems dependent on the resistance mechanism involved.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fuente
Pest Management Science : 1-7 (First published: 12 February 2025)
Materia
Lolium perenne
Glyphosate
Clethodim
Herbicides
Resistance to Herbicides
Weeds
Glifosato
Cletodim
Herbicidas
Resistencia a los Herbicidas
Malezas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21346
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21346
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixtureYanniccari, MarcosGigon, RamonLolium perenneGlyphosateClethodimHerbicidesResistance to HerbicidesWeedsGlifosatoCletodimHerbicidasResistencia a los HerbicidasMalezasBACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart. RESULTS: Plants of the EPSPS overexpression biotype were most sensitive to glyphosate when it was combined with clethodim, and the dose required to halve the maximal response (I50) calculated for glyphosate was reduced to a third. However, the ACCase inhibitor seems to be uniquely responsible for weed control in the P106S biotype. In response to clethodim, EPSPS expression was inhibited in the glyphosate-resistant EPSPS overexpression biotype to a level like or lower than that of susceptible plants. The glyphosate plus clethodim mixture caused a 1.7-fold increase in shikimate concentration in the leaves of susceptible and EPSPS overexpression biotypes compared to glyphosate alone. CONCLUSION: The effects of glyphosate plus clethodim impact in different senses according to the mechanism of resistance involved, where the convergent action of both herbicides controlled the susceptible and EPSPS overexpression biotypes. In contrast, the P106S biotype died because of the clethodim component in the mixture. Mixing herbicides should not be considered a complete solution to herbicide resistance management, and its potential seems dependent on the resistance mechanism involved.EEA BarrowFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Gigón, Ramón. Consultor privado en Control de Malezas; ArgentinaWiley2025-02-19T11:29:34Z2025-02-19T11:29:34Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21346https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.87191526-498X1526-4998https://doi.org/10.1002/ps.8719Pest Management Science : 1-7 (First published: 12 February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-30T11:25:10Zoai:localhost:20.500.12123/21346instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-30 11:25:11.019INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
title Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
spellingShingle Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
Yanniccari, Marcos
Lolium perenne
Glyphosate
Clethodim
Herbicides
Resistance to Herbicides
Weeds
Glifosato
Cletodim
Herbicidas
Resistencia a los Herbicidas
Malezas
title_short Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
title_full Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
title_fullStr Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
title_full_unstemmed Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
title_sort Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
dc.creator.none.fl_str_mv Yanniccari, Marcos
Gigon, Ramon
author Yanniccari, Marcos
author_facet Yanniccari, Marcos
Gigon, Ramon
author_role author
author2 Gigon, Ramon
author2_role author
dc.subject.none.fl_str_mv Lolium perenne
Glyphosate
Clethodim
Herbicides
Resistance to Herbicides
Weeds
Glifosato
Cletodim
Herbicidas
Resistencia a los Herbicidas
Malezas
topic Lolium perenne
Glyphosate
Clethodim
Herbicides
Resistance to Herbicides
Weeds
Glifosato
Cletodim
Herbicidas
Resistencia a los Herbicidas
Malezas
dc.description.none.fl_txt_mv BACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart. RESULTS: Plants of the EPSPS overexpression biotype were most sensitive to glyphosate when it was combined with clethodim, and the dose required to halve the maximal response (I50) calculated for glyphosate was reduced to a third. However, the ACCase inhibitor seems to be uniquely responsible for weed control in the P106S biotype. In response to clethodim, EPSPS expression was inhibited in the glyphosate-resistant EPSPS overexpression biotype to a level like or lower than that of susceptible plants. The glyphosate plus clethodim mixture caused a 1.7-fold increase in shikimate concentration in the leaves of susceptible and EPSPS overexpression biotypes compared to glyphosate alone. CONCLUSION: The effects of glyphosate plus clethodim impact in different senses according to the mechanism of resistance involved, where the convergent action of both herbicides controlled the susceptible and EPSPS overexpression biotypes. In contrast, the P106S biotype died because of the clethodim component in the mixture. Mixing herbicides should not be considered a complete solution to herbicide resistance management, and its potential seems dependent on the resistance mechanism involved.
EEA Barrow
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
description BACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart. RESULTS: Plants of the EPSPS overexpression biotype were most sensitive to glyphosate when it was combined with clethodim, and the dose required to halve the maximal response (I50) calculated for glyphosate was reduced to a third. However, the ACCase inhibitor seems to be uniquely responsible for weed control in the P106S biotype. In response to clethodim, EPSPS expression was inhibited in the glyphosate-resistant EPSPS overexpression biotype to a level like or lower than that of susceptible plants. The glyphosate plus clethodim mixture caused a 1.7-fold increase in shikimate concentration in the leaves of susceptible and EPSPS overexpression biotypes compared to glyphosate alone. CONCLUSION: The effects of glyphosate plus clethodim impact in different senses according to the mechanism of resistance involved, where the convergent action of both herbicides controlled the susceptible and EPSPS overexpression biotypes. In contrast, the P106S biotype died because of the clethodim component in the mixture. Mixing herbicides should not be considered a complete solution to herbicide resistance management, and its potential seems dependent on the resistance mechanism involved.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-19T11:29:34Z
2025-02-19T11:29:34Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21346
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8719
1526-498X
1526-4998
https://doi.org/10.1002/ps.8719
url http://hdl.handle.net/20.500.12123/21346
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8719
https://doi.org/10.1002/ps.8719
identifier_str_mv 1526-498X
1526-4998
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Pest Management Science : 1-7 (First published: 12 February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.589754

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