Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds

Autores
Irazoqui, Jose Matias; Eberhardt, María Florencia; Santiago, Gonzalo Manuel; Amadio, Ariel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent a large segment of industrial enzymes, since they are used in several industries, including food. In this work, we describe three novel enzymes identified using a metagenomic approach. Metagenomic DNA from dairy industry stabilization ponds were sequenced, and the predicted genes were compared against the MEROPS database, focusing on families commercially used to produce whey protein hydrolysates. From a total of 849 candidates, 10 were selected for cloning and expression and three showed activities with both the chromogenic substrate, azocasein, and whey proteins. Particularly, Pr05, an enzyme from the yet uncultured phylum Patescibacteria, showed activity that is comparable to a commercial protease. All these novel enzymes could represent an alternative for dairy industries to produce value-added products from industrial by-products.
ANPCyT PICT 2017–0191, ASACTeI IO-2017–00172
EEA Rafaela
Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Eberhardt, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Santiago, Gonzalo Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Amadio, Ariel​. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Amadio, Ariel​. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fuente
Applied Microbiology and Biotechnology 107 : 4291-4300 (2023)
Materia
Proteases
Metagenomics
Whey Protein
Ponds
Proteasa
Metagenómica
Proteínas de Suero de Leche
Estanque
Whey Protein Hydrolysates
Bioprospection
Hidrolizados de Proteína de Suero
Bioprospección
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18638
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18638
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Characterization of novel proteases identified by metagenomic analysis from dairy stabilization pondsIrazoqui, Jose MatiasEberhardt, María FlorenciaSantiago, Gonzalo ManuelAmadio, ArielProteasesMetagenomicsWhey ProteinPondsProteasaMetagenómicaProteínas de Suero de LecheEstanqueWhey Protein HydrolysatesBioprospectionHidrolizados de Proteína de SueroBioprospecciónCheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent a large segment of industrial enzymes, since they are used in several industries, including food. In this work, we describe three novel enzymes identified using a metagenomic approach. Metagenomic DNA from dairy industry stabilization ponds were sequenced, and the predicted genes were compared against the MEROPS database, focusing on families commercially used to produce whey protein hydrolysates. From a total of 849 candidates, 10 were selected for cloning and expression and three showed activities with both the chromogenic substrate, azocasein, and whey proteins. Particularly, Pr05, an enzyme from the yet uncultured phylum Patescibacteria, showed activity that is comparable to a commercial protease. All these novel enzymes could represent an alternative for dairy industries to produce value-added products from industrial by-products.ANPCyT PICT 2017–0191, ASACTeI IO-2017–00172EEA RafaelaFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Eberhardt, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Santiago, Gonzalo Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Amadio, Ariel​. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Amadio, Ariel​. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaSpringer2024-07-24T12:39:18Z2024-07-24T12:39:18Z2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18638https://link.springer.com/article/10.1007/s00253-023-12591-41432-0614 (electronic)0175-7598https://doi.org/10.1007/s00253-023-12591-4Applied Microbiology and Biotechnology 107 : 4291-4300 (2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I106-001, Estudios metagenómicos en animales y medio ambiente para modular la microbiota, desarrollar probióticos y mitigar el impacto ambiental de la producción pecuariainfo:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicadainfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrialinfo:eu-repograntAgreement/INTA/2019-PE-E7-I150-001, Aprovechamiento de residuos, descartes y subproductos agroalimentarios y agropecuarios: tecnologías para la obtención de alimentos y bioproductos para cadenas productivasinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-18T10:09:34Zoai:localhost:20.500.12123/18638instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:09:35.036INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
title Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
spellingShingle Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
Irazoqui, Jose Matias
Proteases
Metagenomics
Whey Protein
Ponds
Proteasa
Metagenómica
Proteínas de Suero de Leche
Estanque
Whey Protein Hydrolysates
Bioprospection
Hidrolizados de Proteína de Suero
Bioprospección
title_short Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
title_full Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
title_fullStr Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
title_full_unstemmed Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
title_sort Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
dc.creator.none.fl_str_mv Irazoqui, Jose Matias
Eberhardt, María Florencia
Santiago, Gonzalo Manuel
Amadio, Ariel
author Irazoqui, Jose Matias
author_facet Irazoqui, Jose Matias
Eberhardt, María Florencia
Santiago, Gonzalo Manuel
Amadio, Ariel
author_role author
author2 Eberhardt, María Florencia
Santiago, Gonzalo Manuel
Amadio, Ariel
author2_role author
author
author
dc.subject.none.fl_str_mv Proteases
Metagenomics
Whey Protein
Ponds
Proteasa
Metagenómica
Proteínas de Suero de Leche
Estanque
Whey Protein Hydrolysates
Bioprospection
Hidrolizados de Proteína de Suero
Bioprospección
topic Proteases
Metagenomics
Whey Protein
Ponds
Proteasa
Metagenómica
Proteínas de Suero de Leche
Estanque
Whey Protein Hydrolysates
Bioprospection
Hidrolizados de Proteína de Suero
Bioprospección
dc.description.none.fl_txt_mv Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent a large segment of industrial enzymes, since they are used in several industries, including food. In this work, we describe three novel enzymes identified using a metagenomic approach. Metagenomic DNA from dairy industry stabilization ponds were sequenced, and the predicted genes were compared against the MEROPS database, focusing on families commercially used to produce whey protein hydrolysates. From a total of 849 candidates, 10 were selected for cloning and expression and three showed activities with both the chromogenic substrate, azocasein, and whey proteins. Particularly, Pr05, an enzyme from the yet uncultured phylum Patescibacteria, showed activity that is comparable to a commercial protease. All these novel enzymes could represent an alternative for dairy industries to produce value-added products from industrial by-products.
ANPCyT PICT 2017–0191, ASACTeI IO-2017–00172
EEA Rafaela
Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Eberhardt, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Santiago, Gonzalo Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Amadio, Ariel​. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Amadio, Ariel​. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
description Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent a large segment of industrial enzymes, since they are used in several industries, including food. In this work, we describe three novel enzymes identified using a metagenomic approach. Metagenomic DNA from dairy industry stabilization ponds were sequenced, and the predicted genes were compared against the MEROPS database, focusing on families commercially used to produce whey protein hydrolysates. From a total of 849 candidates, 10 were selected for cloning and expression and three showed activities with both the chromogenic substrate, azocasein, and whey proteins. Particularly, Pr05, an enzyme from the yet uncultured phylum Patescibacteria, showed activity that is comparable to a commercial protease. All these novel enzymes could represent an alternative for dairy industries to produce value-added products from industrial by-products.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
2024-07-24T12:39:18Z
2024-07-24T12:39:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18638
https://link.springer.com/article/10.1007/s00253-023-12591-4
1432-0614 (electronic)
0175-7598
https://doi.org/10.1007/s00253-023-12591-4
url http://hdl.handle.net/20.500.12123/18638
https://link.springer.com/article/10.1007/s00253-023-12591-4
https://doi.org/10.1007/s00253-023-12591-4
identifier_str_mv 1432-0614 (electronic)
0175-7598
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I106-001, Estudios metagenómicos en animales y medio ambiente para modular la microbiota, desarrollar probióticos y mitigar el impacto ambiental de la producción pecuaria
info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada
info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial
info:eu-repograntAgreement/INTA/2019-PE-E7-I150-001, Aprovechamiento de residuos, descartes y subproductos agroalimentarios y agropecuarios: tecnologías para la obtención de alimentos y bioproductos para cadenas productivas
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Applied Microbiology and Biotechnology 107 : 4291-4300 (2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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