Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection

Autores
Canal, Ana María; Pezzone, Natalia; Cataldi, Angel Adrian; Zumarraga, Martin Jose; Larzabal, Mariano; Garbaccio, Sergio Gabriel; Fernández, Analía; Dominguez, Lucas; Aranaz, Alicia; Rodriguez-Bertos, Antonio
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.
Inst. de Biotecnología
Fil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
Fil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina
Fil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
Fil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
Fil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España
Fuente
Research in veterinary science 110 : 34-39. (2017)
Materia
Enfermedades de los Animales
Mycobacterium bovis
Ganado Bovino
Citoquinas
Infección por Mycobacterium bovis
Animal Diseases
Cattle
Cytokines
Mycobacterium bovis infections
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/956
Acceder
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oai_identifier_str oai:localhost:20.500.12123/956
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infectionCanal, Ana MaríaPezzone, NataliaCataldi, Angel AdrianZumarraga, Martin JoseLarzabal, MarianoGarbaccio, Sergio GabrielFernández, AnalíaDominguez, LucasAranaz, AliciaRodriguez-Bertos, AntonioEnfermedades de los AnimalesMycobacterium bovisGanado BovinoCitoquinasInfección por Mycobacterium bovisAnimal DiseasesCattleCytokinesMycobacterium bovis infectionsCellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.Inst. de BiotecnologíaFil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; ArgentinaFil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; ArgentinaFil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; EspañaFil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; EspañaFil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España2017-08-11T14:13:26Z2017-08-11T14:13:26Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/956http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa0034-5288https://doi.org/10.1016/j.rvsc.2016.10.006Research in veterinary science 110 : 34-39. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:08Zoai:localhost:20.500.12123/956instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:09.27INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
title Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
spellingShingle Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
Canal, Ana María
Enfermedades de los Animales
Mycobacterium bovis
Ganado Bovino
Citoquinas
Infección por Mycobacterium bovis
Animal Diseases
Cattle
Cytokines
Mycobacterium bovis infections
title_short Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
title_full Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
title_fullStr Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
title_full_unstemmed Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
title_sort Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
dc.creator.none.fl_str_mv Canal, Ana María
Pezzone, Natalia
Cataldi, Angel Adrian
Zumarraga, Martin Jose
Larzabal, Mariano
Garbaccio, Sergio Gabriel
Fernández, Analía
Dominguez, Lucas
Aranaz, Alicia
Rodriguez-Bertos, Antonio
author Canal, Ana María
author_facet Canal, Ana María
Pezzone, Natalia
Cataldi, Angel Adrian
Zumarraga, Martin Jose
Larzabal, Mariano
Garbaccio, Sergio Gabriel
Fernández, Analía
Dominguez, Lucas
Aranaz, Alicia
Rodriguez-Bertos, Antonio
author_role author
author2 Pezzone, Natalia
Cataldi, Angel Adrian
Zumarraga, Martin Jose
Larzabal, Mariano
Garbaccio, Sergio Gabriel
Fernández, Analía
Dominguez, Lucas
Aranaz, Alicia
Rodriguez-Bertos, Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Mycobacterium bovis
Ganado Bovino
Citoquinas
Infección por Mycobacterium bovis
Animal Diseases
Cattle
Cytokines
Mycobacterium bovis infections
topic Enfermedades de los Animales
Mycobacterium bovis
Ganado Bovino
Citoquinas
Infección por Mycobacterium bovis
Animal Diseases
Cattle
Cytokines
Mycobacterium bovis infections
dc.description.none.fl_txt_mv Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.
Inst. de Biotecnología
Fil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
Fil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina
Fil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
Fil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
Fil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España
description Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-11T14:13:26Z
2017-08-11T14:13:26Z
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/956
http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa
0034-5288
https://doi.org/10.1016/j.rvsc.2016.10.006
url http://hdl.handle.net/20.500.12123/956
http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa
https://doi.org/10.1016/j.rvsc.2016.10.006
identifier_str_mv 0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Research in veterinary science 110 : 34-39. (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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