Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
- Autores
- Canal, Ana María; Pezzone, Natalia; Cataldi, Angel Adrian; Zumarraga, Martin Jose; Larzabal, Mariano; Garbaccio, Sergio Gabriel; Fernández, Analía; Dominguez, Lucas; Aranaz, Alicia; Rodriguez-Bertos, Antonio
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.
Inst. de Biotecnología
Fil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
Fil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina
Fil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
Fil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
Fil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España - Fuente
- Research in veterinary science 110 : 34-39. (2017)
- Materia
-
Enfermedades de los Animales
Mycobacterium bovis
Ganado Bovino
Citoquinas
Infección por Mycobacterium bovis
Animal Diseases
Cattle
Cytokines
Mycobacterium bovis infections - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/956
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Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infectionCanal, Ana MaríaPezzone, NataliaCataldi, Angel AdrianZumarraga, Martin JoseLarzabal, MarianoGarbaccio, Sergio GabrielFernández, AnalíaDominguez, LucasAranaz, AliciaRodriguez-Bertos, AntonioEnfermedades de los AnimalesMycobacterium bovisGanado BovinoCitoquinasInfección por Mycobacterium bovisAnimal DiseasesCattleCytokinesMycobacterium bovis infectionsCellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.Inst. de BiotecnologíaFil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; ArgentinaFil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; ArgentinaFil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; EspañaFil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; EspañaFil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España2017-08-11T14:13:26Z2017-08-11T14:13:26Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/956http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa0034-5288https://doi.org/10.1016/j.rvsc.2016.10.006Research in veterinary science 110 : 34-39. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:46:57Zoai:localhost:20.500.12123/956instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:46:58.265INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
title |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
spellingShingle |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection Canal, Ana María Enfermedades de los Animales Mycobacterium bovis Ganado Bovino Citoquinas Infección por Mycobacterium bovis Animal Diseases Cattle Cytokines Mycobacterium bovis infections |
title_short |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
title_full |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
title_fullStr |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
title_full_unstemmed |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
title_sort |
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
dc.creator.none.fl_str_mv |
Canal, Ana María Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio |
author |
Canal, Ana María |
author_facet |
Canal, Ana María Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio |
author_role |
author |
author2 |
Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Enfermedades de los Animales Mycobacterium bovis Ganado Bovino Citoquinas Infección por Mycobacterium bovis Animal Diseases Cattle Cytokines Mycobacterium bovis infections |
topic |
Enfermedades de los Animales Mycobacterium bovis Ganado Bovino Citoquinas Infección por Mycobacterium bovis Animal Diseases Cattle Cytokines Mycobacterium bovis infections |
dc.description.none.fl_txt_mv |
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas. Inst. de Biotecnología Fil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina Fil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina Fil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España Fil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España Fil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España |
description |
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-11T14:13:26Z 2017-08-11T14:13:26Z 2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/956 http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa 0034-5288 https://doi.org/10.1016/j.rvsc.2016.10.006 |
url |
http://hdl.handle.net/20.500.12123/956 http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa https://doi.org/10.1016/j.rvsc.2016.10.006 |
identifier_str_mv |
0034-5288 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Research in veterinary science 110 : 34-39. (2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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