Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)

Autores
Rafi, Nitha; Dominguez, Matías; Mathew, Febina M.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium root rot is a yield-limiting disease of soybean (Glycine max L.) in the United States and Canada (Ontario). Among the species of Fusarium causing root rot, F. proliferatum is a virulent pathogen. Sources of resistance to F. proliferatum have been identified; however, additional screening of soybean accessions is necessary to identify quantitative trait loci (QTLs) associated with resistance to F. proliferatum. The objective of this study was to evaluate 268 soybean accessions obtained from the USDA Germplasm Collection belonging to maturity groups 000 to IX for resistance to a single isolate of F. proliferatum under greenhouse conditions. Additionally, the study sought to identify QTLs, single-nucleotide polymorphism (SNP) markers, and candidate genes associated with the F. proliferatum resistance through a genome-wide association study (GWAS). The experiment was conducted in a completely randomized design using a layer inoculation method and repeated once. The root rot severity was assessed 21 days postinoculation and expressed as the relative treatment effect (RTE). Fifty-two accessions had a significantly lower RTE compared with the susceptible variety ‘Williams 82’ (ATS = 37.03; df = 7.30; P = 2.47 × 10⁻⁵⁴). GWAS analysis using 36,071 SNP markers identified one major QTL on chromosome 11 that explained 30.95% of the phenotype variance, three strongly associated SNP markers, and three candidate genes that could be involved in resistance to F. proliferatum. This study identified soybean accessions with resistance to F. proliferatum, along with novel SNP markers, which could significantly enhance breeding programs aimed at developing cultivars with resistance to Fusarium root rot.
EEA Pergamino
Fil: Rafi, Nitha. North Dakota State University. Department of Plant Pathology; Estados Unidos
Fil: Dominguez, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sector Girasol; Argentina
Fil: Mathew, Febina Merlin. North Dakota State University. Department of Plant Pathology; Estados Unidos
Fuente
Plant Health Progress : journal of applied plant health : 1-7. (September 2025)
Materia
Enfermedades de las Plantas
Hongos
Fusarium proliferatum
Soja
Resistencia a las Plagas
Plant Diseases
Fungi
Soybeans
Genes
Pest Resistance
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Host Resistance
Root Rot
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23843
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oai_identifier_str oai:localhost:20.500.12123/23843
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)Rafi, NithaDominguez, MatíasMathew, Febina M.Enfermedades de las PlantasHongosFusarium proliferatumSojaResistencia a las PlagasPlant DiseasesFungiSoybeansGenesPest ResistanceQuantitative Trait LociLoci de Rasgos CuantitativosHost ResistanceRoot RotFusarium root rot is a yield-limiting disease of soybean (Glycine max L.) in the United States and Canada (Ontario). Among the species of Fusarium causing root rot, F. proliferatum is a virulent pathogen. Sources of resistance to F. proliferatum have been identified; however, additional screening of soybean accessions is necessary to identify quantitative trait loci (QTLs) associated with resistance to F. proliferatum. The objective of this study was to evaluate 268 soybean accessions obtained from the USDA Germplasm Collection belonging to maturity groups 000 to IX for resistance to a single isolate of F. proliferatum under greenhouse conditions. Additionally, the study sought to identify QTLs, single-nucleotide polymorphism (SNP) markers, and candidate genes associated with the F. proliferatum resistance through a genome-wide association study (GWAS). The experiment was conducted in a completely randomized design using a layer inoculation method and repeated once. The root rot severity was assessed 21 days postinoculation and expressed as the relative treatment effect (RTE). Fifty-two accessions had a significantly lower RTE compared with the susceptible variety ‘Williams 82’ (ATS = 37.03; df = 7.30; P = 2.47 × 10⁻⁵⁴). GWAS analysis using 36,071 SNP markers identified one major QTL on chromosome 11 that explained 30.95% of the phenotype variance, three strongly associated SNP markers, and three candidate genes that could be involved in resistance to F. proliferatum. This study identified soybean accessions with resistance to F. proliferatum, along with novel SNP markers, which could significantly enhance breeding programs aimed at developing cultivars with resistance to Fusarium root rot.EEA PergaminoFil: Rafi, Nitha. North Dakota State University. Department of Plant Pathology; Estados UnidosFil: Dominguez, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sector Girasol; ArgentinaFil: Mathew, Febina Merlin. North Dakota State University. Department of Plant Pathology; Estados UnidosAmerican Phytopathological Society2025-09-18T10:27:33Z2025-09-18T10:27:33Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23843https://apsjournals.apsnet.org/doi/10.1094/PHP-03-25-0100-RS1535-1025 (online)https://doi.org/10.1094/PHP-03-25-0100-RSPlant Health Progress : journal of applied plant health : 1-7. (September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:35Zoai:localhost:20.500.12123/23843instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:35.804INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
title Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
spellingShingle Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
Rafi, Nitha
Enfermedades de las Plantas
Hongos
Fusarium proliferatum
Soja
Resistencia a las Plagas
Plant Diseases
Fungi
Soybeans
Genes
Pest Resistance
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Host Resistance
Root Rot
title_short Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
title_full Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
title_fullStr Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
title_full_unstemmed Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
title_sort Genome-wide association study identified one major quantitative trait locus associated with resistance to Fusarium proliferatum in soybean (Glycine max)
dc.creator.none.fl_str_mv Rafi, Nitha
Dominguez, Matías
Mathew, Febina M.
author Rafi, Nitha
author_facet Rafi, Nitha
Dominguez, Matías
Mathew, Febina M.
author_role author
author2 Dominguez, Matías
Mathew, Febina M.
author2_role author
author
dc.subject.none.fl_str_mv Enfermedades de las Plantas
Hongos
Fusarium proliferatum
Soja
Resistencia a las Plagas
Plant Diseases
Fungi
Soybeans
Genes
Pest Resistance
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Host Resistance
Root Rot
topic Enfermedades de las Plantas
Hongos
Fusarium proliferatum
Soja
Resistencia a las Plagas
Plant Diseases
Fungi
Soybeans
Genes
Pest Resistance
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Host Resistance
Root Rot
dc.description.none.fl_txt_mv Fusarium root rot is a yield-limiting disease of soybean (Glycine max L.) in the United States and Canada (Ontario). Among the species of Fusarium causing root rot, F. proliferatum is a virulent pathogen. Sources of resistance to F. proliferatum have been identified; however, additional screening of soybean accessions is necessary to identify quantitative trait loci (QTLs) associated with resistance to F. proliferatum. The objective of this study was to evaluate 268 soybean accessions obtained from the USDA Germplasm Collection belonging to maturity groups 000 to IX for resistance to a single isolate of F. proliferatum under greenhouse conditions. Additionally, the study sought to identify QTLs, single-nucleotide polymorphism (SNP) markers, and candidate genes associated with the F. proliferatum resistance through a genome-wide association study (GWAS). The experiment was conducted in a completely randomized design using a layer inoculation method and repeated once. The root rot severity was assessed 21 days postinoculation and expressed as the relative treatment effect (RTE). Fifty-two accessions had a significantly lower RTE compared with the susceptible variety ‘Williams 82’ (ATS = 37.03; df = 7.30; P = 2.47 × 10⁻⁵⁴). GWAS analysis using 36,071 SNP markers identified one major QTL on chromosome 11 that explained 30.95% of the phenotype variance, three strongly associated SNP markers, and three candidate genes that could be involved in resistance to F. proliferatum. This study identified soybean accessions with resistance to F. proliferatum, along with novel SNP markers, which could significantly enhance breeding programs aimed at developing cultivars with resistance to Fusarium root rot.
EEA Pergamino
Fil: Rafi, Nitha. North Dakota State University. Department of Plant Pathology; Estados Unidos
Fil: Dominguez, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sector Girasol; Argentina
Fil: Mathew, Febina Merlin. North Dakota State University. Department of Plant Pathology; Estados Unidos
description Fusarium root rot is a yield-limiting disease of soybean (Glycine max L.) in the United States and Canada (Ontario). Among the species of Fusarium causing root rot, F. proliferatum is a virulent pathogen. Sources of resistance to F. proliferatum have been identified; however, additional screening of soybean accessions is necessary to identify quantitative trait loci (QTLs) associated with resistance to F. proliferatum. The objective of this study was to evaluate 268 soybean accessions obtained from the USDA Germplasm Collection belonging to maturity groups 000 to IX for resistance to a single isolate of F. proliferatum under greenhouse conditions. Additionally, the study sought to identify QTLs, single-nucleotide polymorphism (SNP) markers, and candidate genes associated with the F. proliferatum resistance through a genome-wide association study (GWAS). The experiment was conducted in a completely randomized design using a layer inoculation method and repeated once. The root rot severity was assessed 21 days postinoculation and expressed as the relative treatment effect (RTE). Fifty-two accessions had a significantly lower RTE compared with the susceptible variety ‘Williams 82’ (ATS = 37.03; df = 7.30; P = 2.47 × 10⁻⁵⁴). GWAS analysis using 36,071 SNP markers identified one major QTL on chromosome 11 that explained 30.95% of the phenotype variance, three strongly associated SNP markers, and three candidate genes that could be involved in resistance to F. proliferatum. This study identified soybean accessions with resistance to F. proliferatum, along with novel SNP markers, which could significantly enhance breeding programs aimed at developing cultivars with resistance to Fusarium root rot.
publishDate 2025
dc.date.none.fl_str_mv 2025-09-18T10:27:33Z
2025-09-18T10:27:33Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23843
https://apsjournals.apsnet.org/doi/10.1094/PHP-03-25-0100-RS
1535-1025 (online)
https://doi.org/10.1094/PHP-03-25-0100-RS
url http://hdl.handle.net/20.500.12123/23843
https://apsjournals.apsnet.org/doi/10.1094/PHP-03-25-0100-RS
https://doi.org/10.1094/PHP-03-25-0100-RS
identifier_str_mv 1535-1025 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Plant Health Progress : journal of applied plant health : 1-7. (September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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