Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants

Autores
Armendariz, Ana Laura; Talano, Melina Andrea; Wevar Oller, Ana Laura; Medina, Maria Ines; Agostini, Elizabeth
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacterial ability to colonize the rhizosphere of plants in arsenic (As) contaminated soils is highly important for symbiotic and free-living plant growth-promoting rhizobacteria (PGPR) used as inoculants, since they can contribute to enhance plant As tolerance and limit metalloid uptake by plants. The aim of this work was to study the effect of As on growth, exopolysaccharide (EPS) production, biofilm formation and motility of two strains used as soybean inoculants, Bradyrhizobium japonicum E109 and Azospirillum brasilense Az39. The metabolism of arsenate (As(V)) and arsenite (As(III)) and their removal and/or possible accumulation were also evaluated. The behavior of both bacteria under As treatment was compared and discussed in relation to their potential for colonizing plant rhizosphere with high content of the metalloid. B. japonicum E109 growth was reduced with As(III) concentration from 10. μM while A. brasilense Az39 showed a reduction of growth with As(III) from 500. μM. EPS and biofilm production increased significantly under 25. μM As(III) for both strains. Moreover, this was more notorious for Azospirillum under 500. μM As(III), where motility was seriously affected. Both bacterial strains showed a similar ability to reduce As(V). However, Azospirillum was able to oxidize more As(III) (around 53%) than Bradyrhizobium (17%). In addition, both strains accumulated As in cell biomass. The behavior of Azospirillum under As treatments suggests that this strain would be able to colonize efficiently As contaminated soils. In this way, inoculation with A. brasilense Az39 would positively contribute to promoting growth of different plant species under As treatment.
Fil: Armendariz, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wevar Oller, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Maria Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Agostini, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Río Cuarto; Argentina
Materia
ACCUMULATION
ARSENIC
BIOFILM
COLONIZATION
EXOPOLYSACCHARIDES
PLANT GROWTH-PROMOTING RHIZOBACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61781
Acceder
id CONICETDig_7d0a253417047ae1990cfe8fd1b0b50c
oai_identifier_str oai:ri.conicet.gov.ar:11336/61781
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculantsArmendariz, Ana LauraTalano, Melina AndreaWevar Oller, Ana LauraMedina, Maria InesAgostini, ElizabethACCUMULATIONARSENICBIOFILMCOLONIZATIONEXOPOLYSACCHARIDESPLANT GROWTH-PROMOTING RHIZOBACTERIAhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Bacterial ability to colonize the rhizosphere of plants in arsenic (As) contaminated soils is highly important for symbiotic and free-living plant growth-promoting rhizobacteria (PGPR) used as inoculants, since they can contribute to enhance plant As tolerance and limit metalloid uptake by plants. The aim of this work was to study the effect of As on growth, exopolysaccharide (EPS) production, biofilm formation and motility of two strains used as soybean inoculants, Bradyrhizobium japonicum E109 and Azospirillum brasilense Az39. The metabolism of arsenate (As(V)) and arsenite (As(III)) and their removal and/or possible accumulation were also evaluated. The behavior of both bacteria under As treatment was compared and discussed in relation to their potential for colonizing plant rhizosphere with high content of the metalloid. B. japonicum E109 growth was reduced with As(III) concentration from 10. μM while A. brasilense Az39 showed a reduction of growth with As(III) from 500. μM. EPS and biofilm production increased significantly under 25. μM As(III) for both strains. Moreover, this was more notorious for Azospirillum under 500. μM As(III), where motility was seriously affected. Both bacterial strains showed a similar ability to reduce As(V). However, Azospirillum was able to oxidize more As(III) (around 53%) than Bradyrhizobium (17%). In addition, both strains accumulated As in cell biomass. The behavior of Azospirillum under As treatments suggests that this strain would be able to colonize efficiently As contaminated soils. In this way, inoculation with A. brasilense Az39 would positively contribute to promoting growth of different plant species under As treatment.Fil: Armendariz, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wevar Oller, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medina, Maria Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Agostini, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Río Cuarto; ArgentinaScience Press2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61781Armendariz, Ana Laura; Talano, Melina Andrea; Wevar Oller, Ana Laura; Medina, Maria Ines; Agostini, Elizabeth; Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants; Science Press; Journal of Environmental Sciences; 33; 7-2015; 203-2101001-0742CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jes.2014.12.024info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1001074215001618info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:25Zoai:ri.conicet.gov.ar:11336/61781instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:25.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
title Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
spellingShingle Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
Armendariz, Ana Laura
ACCUMULATION
ARSENIC
BIOFILM
COLONIZATION
EXOPOLYSACCHARIDES
PLANT GROWTH-PROMOTING RHIZOBACTERIA
title_short Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
title_full Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
title_fullStr Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
title_full_unstemmed Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
title_sort Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants
dc.creator.none.fl_str_mv Armendariz, Ana Laura
Talano, Melina Andrea
Wevar Oller, Ana Laura
Medina, Maria Ines
Agostini, Elizabeth
author Armendariz, Ana Laura
author_facet Armendariz, Ana Laura
Talano, Melina Andrea
Wevar Oller, Ana Laura
Medina, Maria Ines
Agostini, Elizabeth
author_role author
author2 Talano, Melina Andrea
Wevar Oller, Ana Laura
Medina, Maria Ines
Agostini, Elizabeth
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACCUMULATION
ARSENIC
BIOFILM
COLONIZATION
EXOPOLYSACCHARIDES
PLANT GROWTH-PROMOTING RHIZOBACTERIA
topic ACCUMULATION
ARSENIC
BIOFILM
COLONIZATION
EXOPOLYSACCHARIDES
PLANT GROWTH-PROMOTING RHIZOBACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacterial ability to colonize the rhizosphere of plants in arsenic (As) contaminated soils is highly important for symbiotic and free-living plant growth-promoting rhizobacteria (PGPR) used as inoculants, since they can contribute to enhance plant As tolerance and limit metalloid uptake by plants. The aim of this work was to study the effect of As on growth, exopolysaccharide (EPS) production, biofilm formation and motility of two strains used as soybean inoculants, Bradyrhizobium japonicum E109 and Azospirillum brasilense Az39. The metabolism of arsenate (As(V)) and arsenite (As(III)) and their removal and/or possible accumulation were also evaluated. The behavior of both bacteria under As treatment was compared and discussed in relation to their potential for colonizing plant rhizosphere with high content of the metalloid. B. japonicum E109 growth was reduced with As(III) concentration from 10. μM while A. brasilense Az39 showed a reduction of growth with As(III) from 500. μM. EPS and biofilm production increased significantly under 25. μM As(III) for both strains. Moreover, this was more notorious for Azospirillum under 500. μM As(III), where motility was seriously affected. Both bacterial strains showed a similar ability to reduce As(V). However, Azospirillum was able to oxidize more As(III) (around 53%) than Bradyrhizobium (17%). In addition, both strains accumulated As in cell biomass. The behavior of Azospirillum under As treatments suggests that this strain would be able to colonize efficiently As contaminated soils. In this way, inoculation with A. brasilense Az39 would positively contribute to promoting growth of different plant species under As treatment.
Fil: Armendariz, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wevar Oller, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Maria Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Agostini, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Río Cuarto; Argentina
description Bacterial ability to colonize the rhizosphere of plants in arsenic (As) contaminated soils is highly important for symbiotic and free-living plant growth-promoting rhizobacteria (PGPR) used as inoculants, since they can contribute to enhance plant As tolerance and limit metalloid uptake by plants. The aim of this work was to study the effect of As on growth, exopolysaccharide (EPS) production, biofilm formation and motility of two strains used as soybean inoculants, Bradyrhizobium japonicum E109 and Azospirillum brasilense Az39. The metabolism of arsenate (As(V)) and arsenite (As(III)) and their removal and/or possible accumulation were also evaluated. The behavior of both bacteria under As treatment was compared and discussed in relation to their potential for colonizing plant rhizosphere with high content of the metalloid. B. japonicum E109 growth was reduced with As(III) concentration from 10. μM while A. brasilense Az39 showed a reduction of growth with As(III) from 500. μM. EPS and biofilm production increased significantly under 25. μM As(III) for both strains. Moreover, this was more notorious for Azospirillum under 500. μM As(III), where motility was seriously affected. Both bacterial strains showed a similar ability to reduce As(V). However, Azospirillum was able to oxidize more As(III) (around 53%) than Bradyrhizobium (17%). In addition, both strains accumulated As in cell biomass. The behavior of Azospirillum under As treatments suggests that this strain would be able to colonize efficiently As contaminated soils. In this way, inoculation with A. brasilense Az39 would positively contribute to promoting growth of different plant species under As treatment.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61781
Armendariz, Ana Laura; Talano, Melina Andrea; Wevar Oller, Ana Laura; Medina, Maria Ines; Agostini, Elizabeth; Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants; Science Press; Journal of Environmental Sciences; 33; 7-2015; 203-210
1001-0742
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61781
identifier_str_mv Armendariz, Ana Laura; Talano, Melina Andrea; Wevar Oller, Ana Laura; Medina, Maria Ines; Agostini, Elizabeth; Effect of arsenic on tolerance mechanisms of two plant growth-promoting bacteria used as biological inoculants; Science Press; Journal of Environmental Sciences; 33; 7-2015; 203-210
1001-0742
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jes.2014.12.024
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1001074215001618
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Press
publisher.none.fl_str_mv Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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