Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum

Autores
Lomonaco, Marina; Sowul, Mariana; Gutierrez, Gerónimo; Malacari, Darío Amilcar; Alvarez, Irene; Porta, Natalia Gabriela; Zabal, Osvaldo Alfredo; Trono, Karina Gabriela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spray-dried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated.
Instituto de Virología
Fil: Lomonaco, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Sowul, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Porta, Natalia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Sowul, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Porta, Natalia Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fuente
Journal of dairy science 103 (7) : 6504-6510. (Julio 2020)
Materia
Bovine Leukaemia Virus
Colostrum
Spray Drying
Virus Leucemia Bovina
Calostro
Secado por Pulverización
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8601
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8601
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network_name_str INTA Digital (INTA)
spelling Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrumLomonaco, MarinaSowul, MarianaGutierrez, GerónimoMalacari, Darío AmilcarAlvarez, IrenePorta, Natalia GabrielaZabal, Osvaldo AlfredoTrono, Karina GabrielaBovine Leukaemia VirusColostrumSpray DryingVirus Leucemia BovinaCalostroSecado por PulverizaciónPrevious studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spray-dried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated.Instituto de VirologíaFil: Lomonaco, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Sowul, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Porta, Natalia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Sowul, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaFil: Porta, Natalia Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaFil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaElsevier2021-01-15T13:11:01Z2021-01-15T13:11:01Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8601https://www.sciencedirect.com/science/article/abs/pii/S00220302203034901525-3198https://doi.org/10.3168/jds.2019-17854Journal of dairy science 103 (7) : 6504-6510. (Julio 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.info:eu-repo/semantics/restrictedAccess2025-09-04T09:48:45Zoai:localhost:20.500.12123/8601instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:45.813INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
title Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
spellingShingle Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
Lomonaco, Marina
Bovine Leukaemia Virus
Colostrum
Spray Drying
Virus Leucemia Bovina
Calostro
Secado por Pulverización
title_short Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
title_full Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
title_fullStr Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
title_full_unstemmed Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
title_sort Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
dc.creator.none.fl_str_mv Lomonaco, Marina
Sowul, Mariana
Gutierrez, Gerónimo
Malacari, Darío Amilcar
Alvarez, Irene
Porta, Natalia Gabriela
Zabal, Osvaldo Alfredo
Trono, Karina Gabriela
author Lomonaco, Marina
author_facet Lomonaco, Marina
Sowul, Mariana
Gutierrez, Gerónimo
Malacari, Darío Amilcar
Alvarez, Irene
Porta, Natalia Gabriela
Zabal, Osvaldo Alfredo
Trono, Karina Gabriela
author_role author
author2 Sowul, Mariana
Gutierrez, Gerónimo
Malacari, Darío Amilcar
Alvarez, Irene
Porta, Natalia Gabriela
Zabal, Osvaldo Alfredo
Trono, Karina Gabriela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovine Leukaemia Virus
Colostrum
Spray Drying
Virus Leucemia Bovina
Calostro
Secado por Pulverización
topic Bovine Leukaemia Virus
Colostrum
Spray Drying
Virus Leucemia Bovina
Calostro
Secado por Pulverización
dc.description.none.fl_txt_mv Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spray-dried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated.
Instituto de Virología
Fil: Lomonaco, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Sowul, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Porta, Natalia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Sowul, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Porta, Natalia Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
description Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spray-dried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
2021-01-15T13:11:01Z
2021-01-15T13:11:01Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8601
https://www.sciencedirect.com/science/article/abs/pii/S0022030220303490
1525-3198
https://doi.org/10.3168/jds.2019-17854
url http://hdl.handle.net/20.500.12123/8601
https://www.sciencedirect.com/science/article/abs/pii/S0022030220303490
https://doi.org/10.3168/jds.2019-17854
identifier_str_mv 1525-3198
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of dairy science 103 (7) : 6504-6510. (Julio 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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