Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves

Autores
De Haro, Luis Alejandro; Arellano, Sofía Maité; Novak, Ondrej; Feil, Regina; Dumon, Analia Delfina; Mattio, Maria Fernanda; Tarkowska, Danuse; Llauger, Gabriela; Strnad, Miroslav; Lunn, John Edward; Pearce, Stephen; Figueroa, Carlos María; Del Vas, Mariana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.
Instituto de Biotecnología
Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Novak, Ondrej. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa
Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Dumon, Analia Delina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Tarkowska, Danuse. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa
Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strnad, Miroslav. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa
Fil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidos
Fil: Figueroa, Carlos María. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
BMC Plant Biology 19 : 112 (Marzo 2019)
Materia
Symptoms
Fijivirus
Sustancias de Crecimiento Vegetal
Reoviridae
Trehalosa
Trigo
Síntomas
Plant Growth Substances
Trehalose
Wheat
Mal de Río Cuarto virus
Plant Hormones
Sucrose Metabolism
Virus del mal de Río Cuarto
Hormonas Vegetales
Metabolismo de la Sacarosa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4806
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4806
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leavesDe Haro, Luis AlejandroArellano, Sofía MaitéNovak, OndrejFeil, ReginaDumon, Analia DelfinaMattio, Maria FernandaTarkowska, DanuseLlauger, GabrielaStrnad, MiroslavLunn, John EdwardPearce, StephenFigueroa, Carlos MaríaDel Vas, MarianaSymptomsFijivirusSustancias de Crecimiento VegetalReoviridaeTrehalosaTrigoSíntomasPlant Growth SubstancesTrehaloseWheatMal de Río Cuarto virusPlant HormonesSucrose MetabolismVirus del mal de Río CuartoHormonas VegetalesMetabolismo de la SacarosaBackground; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.Instituto de BiotecnologíaFil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Novak, Ondrej. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República ChecaFil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Dumon, Analia Delina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Tarkowska, Danuse. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República ChecaFil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Strnad, Miroslav. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República ChecaFil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados UnidosFil: Figueroa, Carlos María. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBMC2019-04-03T16:43:30Z2019-04-03T16:43:30Z2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4806https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y1471-2229https://doi.org/10.1186/s12870-019-1709-yBMC Plant Biology 19 : 112 (Marzo 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO/1131022/AR./Genómica funcional y biología de sistemas.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:37Zoai:localhost:20.500.12123/4806instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:37.61INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
title Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
spellingShingle Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
De Haro, Luis Alejandro
Symptoms
Fijivirus
Sustancias de Crecimiento Vegetal
Reoviridae
Trehalosa
Trigo
Síntomas
Plant Growth Substances
Trehalose
Wheat
Mal de Río Cuarto virus
Plant Hormones
Sucrose Metabolism
Virus del mal de Río Cuarto
Hormonas Vegetales
Metabolismo de la Sacarosa
title_short Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
title_full Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
title_fullStr Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
title_full_unstemmed Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
title_sort Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
dc.creator.none.fl_str_mv De Haro, Luis Alejandro
Arellano, Sofía Maité
Novak, Ondrej
Feil, Regina
Dumon, Analia Delfina
Mattio, Maria Fernanda
Tarkowska, Danuse
Llauger, Gabriela
Strnad, Miroslav
Lunn, John Edward
Pearce, Stephen
Figueroa, Carlos María
Del Vas, Mariana
author De Haro, Luis Alejandro
author_facet De Haro, Luis Alejandro
Arellano, Sofía Maité
Novak, Ondrej
Feil, Regina
Dumon, Analia Delfina
Mattio, Maria Fernanda
Tarkowska, Danuse
Llauger, Gabriela
Strnad, Miroslav
Lunn, John Edward
Pearce, Stephen
Figueroa, Carlos María
Del Vas, Mariana
author_role author
author2 Arellano, Sofía Maité
Novak, Ondrej
Feil, Regina
Dumon, Analia Delfina
Mattio, Maria Fernanda
Tarkowska, Danuse
Llauger, Gabriela
Strnad, Miroslav
Lunn, John Edward
Pearce, Stephen
Figueroa, Carlos María
Del Vas, Mariana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Symptoms
Fijivirus
Sustancias de Crecimiento Vegetal
Reoviridae
Trehalosa
Trigo
Síntomas
Plant Growth Substances
Trehalose
Wheat
Mal de Río Cuarto virus
Plant Hormones
Sucrose Metabolism
Virus del mal de Río Cuarto
Hormonas Vegetales
Metabolismo de la Sacarosa
topic Symptoms
Fijivirus
Sustancias de Crecimiento Vegetal
Reoviridae
Trehalosa
Trigo
Síntomas
Plant Growth Substances
Trehalose
Wheat
Mal de Río Cuarto virus
Plant Hormones
Sucrose Metabolism
Virus del mal de Río Cuarto
Hormonas Vegetales
Metabolismo de la Sacarosa
dc.description.none.fl_txt_mv Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.
Instituto de Biotecnología
Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Novak, Ondrej. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa
Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Dumon, Analia Delina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Tarkowska, Danuse. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa
Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strnad, Miroslav. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checa
Fil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidos
Fil: Figueroa, Carlos María. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-03T16:43:30Z
2019-04-03T16:43:30Z
2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4806
https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y
1471-2229
https://doi.org/10.1186/s12870-019-1709-y
url http://hdl.handle.net/20.500.12123/4806
https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y
https://doi.org/10.1186/s12870-019-1709-y
identifier_str_mv 1471-2229
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO/1131022/AR./Genómica funcional y biología de sistemas.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv BMC Plant Biology 19 : 112 (Marzo 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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