Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
- Autores
- de Haro, Luis Alejandro; Arellano, Sofía Maité; Novák, Ondrej; Feil, Regina; Dumón, Analía Delina; Mattio, Maria Fernanda; Tarkowská, Danuse; Llauger, Gabriela; Strnad, Miroslav; Lunn, John E.; Pearce, Stephen; Figueroa, Carlos Maria; del Vas, Mariana
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.
Fil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Novák, Ondrej. Univerzita Palack; República Checa
Fil: Feil, Regina. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Dumón, Analía Delina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Tarkowská, Danuse. Univerzita Palack; República Checa
Fil: Llauger, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strnad, Miroslav. Univerzita Palack; República Checa
Fil: Lunn, John E.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Pearce, Stephen. State University of Colorado - Fort Collins; Estados Unidos
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina - Materia
-
DISEASE SYMPTOMS
FIJIVIRUS
MRCV
PLANT HORMONES
REOVIRIDAE
SUCROSE METABOLISM
SWEET TRANSPORTERS
TREHALOSE 6-PHOSPHATE
WHEAT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/118743
Ver los metadatos del registro completo
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Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leavesde Haro, Luis AlejandroArellano, Sofía MaitéNovák, OndrejFeil, ReginaDumón, Analía DelinaMattio, Maria FernandaTarkowská, DanuseLlauger, GabrielaStrnad, MiroslavLunn, John E.Pearce, StephenFigueroa, Carlos Mariadel Vas, MarianaDISEASE SYMPTOMSFIJIVIRUSMRCVPLANT HORMONESREOVIRIDAESUCROSE METABOLISMSWEET TRANSPORTERSTREHALOSE 6-PHOSPHATEWHEAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.Fil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Novák, Ondrej. Univerzita Palack; República ChecaFil: Feil, Regina. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Dumón, Analía Delina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Tarkowská, Danuse. Univerzita Palack; República ChecaFil: Llauger, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Strnad, Miroslav. Univerzita Palack; República ChecaFil: Lunn, John E.. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Pearce, Stephen. State University of Colorado - Fort Collins; Estados UnidosFil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaBioMed Central2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118743de Haro, Luis Alejandro; Arellano, Sofía Maité; Novák, Ondrej; Feil, Regina; Dumón, Analía Delina; et al.; Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves; BioMed Central; BMC Plant Biology; 19; 1; 3-20191471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-019-1709-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:35Zoai:ri.conicet.gov.ar:11336/118743instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:35.943CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| spellingShingle |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves de Haro, Luis Alejandro DISEASE SYMPTOMS FIJIVIRUS MRCV PLANT HORMONES REOVIRIDAE SUCROSE METABOLISM SWEET TRANSPORTERS TREHALOSE 6-PHOSPHATE WHEAT |
| title_short |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_full |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_fullStr |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_full_unstemmed |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| title_sort |
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves |
| dc.creator.none.fl_str_mv |
de Haro, Luis Alejandro Arellano, Sofía Maité Novák, Ondrej Feil, Regina Dumón, Analía Delina Mattio, Maria Fernanda Tarkowská, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John E. Pearce, Stephen Figueroa, Carlos Maria del Vas, Mariana |
| author |
de Haro, Luis Alejandro |
| author_facet |
de Haro, Luis Alejandro Arellano, Sofía Maité Novák, Ondrej Feil, Regina Dumón, Analía Delina Mattio, Maria Fernanda Tarkowská, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John E. Pearce, Stephen Figueroa, Carlos Maria del Vas, Mariana |
| author_role |
author |
| author2 |
Arellano, Sofía Maité Novák, Ondrej Feil, Regina Dumón, Analía Delina Mattio, Maria Fernanda Tarkowská, Danuse Llauger, Gabriela Strnad, Miroslav Lunn, John E. Pearce, Stephen Figueroa, Carlos Maria del Vas, Mariana |
| author2_role |
author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
DISEASE SYMPTOMS FIJIVIRUS MRCV PLANT HORMONES REOVIRIDAE SUCROSE METABOLISM SWEET TRANSPORTERS TREHALOSE 6-PHOSPHATE WHEAT |
| topic |
DISEASE SYMPTOMS FIJIVIRUS MRCV PLANT HORMONES REOVIRIDAE SUCROSE METABOLISM SWEET TRANSPORTERS TREHALOSE 6-PHOSPHATE WHEAT |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Background: Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants. Fil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Novák, Ondrej. Univerzita Palack; República Checa Fil: Feil, Regina. Max Planck Institute Of Molecular Plant Physiology; Alemania Fil: Dumón, Analía Delina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Tarkowská, Danuse. Univerzita Palack; República Checa Fil: Llauger, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Strnad, Miroslav. Univerzita Palack; República Checa Fil: Lunn, John E.. Max Planck Institute Of Molecular Plant Physiology; Alemania Fil: Pearce, Stephen. State University of Colorado - Fort Collins; Estados Unidos Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina |
| description |
Background: Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-03 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://hdl.handle.net/11336/118743 de Haro, Luis Alejandro; Arellano, Sofía Maité; Novák, Ondrej; Feil, Regina; Dumón, Analía Delina; et al.; Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves; BioMed Central; BMC Plant Biology; 19; 1; 3-2019 1471-2229 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/118743 |
| identifier_str_mv |
de Haro, Luis Alejandro; Arellano, Sofía Maité; Novák, Ondrej; Feil, Regina; Dumón, Analía Delina; et al.; Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves; BioMed Central; BMC Plant Biology; 19; 1; 3-2019 1471-2229 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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BioMed Central |
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BioMed Central |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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