Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector

Autores
De Haro, Luis Alejandro; Dumon, Analia Delina; Mattio, Maria Fernanda; Arguello Caro, Evangelina Beatriz; Llauger, Gabriela; Zavallo, Diego; Blanc, Hervé; Mongelli, Vanesa Claudia; Truol, Graciela Ana Maria; Saleh, María-Carla; Asurmendi, Sebastian; Del Vas, Mariana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.
Inst. de Biotecnología
Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dumon, Analia Delina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Blanc, Hervé. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia
Fil: Truol, Graciela Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Mongelli, Vanesa Claudia. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia
Fil: Saleh, María-Carla. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontiers in plant science 8 :766. (May 2017)
Materia
ARN
Virus de las Plantas
Trigo
Fulgoroidea
Plant Viruses
Wheat
RNA
Virus del Mal de Río Cuarto
Fijivirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1108
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1108
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vectorDe Haro, Luis AlejandroDumon, Analia DelinaMattio, Maria FernandaArguello Caro, Evangelina BeatrizLlauger, GabrielaZavallo, DiegoBlanc, HervéMongelli, Vanesa ClaudiaTruol, Graciela Ana MariaSaleh, María-CarlaAsurmendi, SebastianDel Vas, MarianaARNVirus de las PlantasTrigoFulgoroideaPlant VirusesWheatRNAVirus del Mal de Río CuartoFijivirusPlant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.Inst. de BiotecnologíaFil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dumon, Analia Delina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Blanc, Hervé. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; FranciaFil: Truol, Graciela Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Mongelli, Vanesa Claudia. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; FranciaFil: Saleh, María-Carla. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; FranciaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-09-01T18:02:06Z2017-09-01T18:02:06Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1108http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/fullde Haro LA, Dumón AD, Mattio MF, Argüello Caro EB, Llauger G, Zavallo D, Blanc H, Mongelli VC, Truol G, Saleh M-C, Asurmendi S and del Vas M (2017) Mal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper Vector. Front. Plant Sci. 8:766https://doi.org/10.3389/fpls.2017.00766Frontiers in plant science 8 :766. (May 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:46:58Zoai:localhost:20.500.12123/1108instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:46:59.667INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
title Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
spellingShingle Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
De Haro, Luis Alejandro
ARN
Virus de las Plantas
Trigo
Fulgoroidea
Plant Viruses
Wheat
RNA
Virus del Mal de Río Cuarto
Fijivirus
title_short Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
title_full Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
title_fullStr Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
title_full_unstemmed Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
title_sort Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
dc.creator.none.fl_str_mv De Haro, Luis Alejandro
Dumon, Analia Delina
Mattio, Maria Fernanda
Arguello Caro, Evangelina Beatriz
Llauger, Gabriela
Zavallo, Diego
Blanc, Hervé
Mongelli, Vanesa Claudia
Truol, Graciela Ana Maria
Saleh, María-Carla
Asurmendi, Sebastian
Del Vas, Mariana
author De Haro, Luis Alejandro
author_facet De Haro, Luis Alejandro
Dumon, Analia Delina
Mattio, Maria Fernanda
Arguello Caro, Evangelina Beatriz
Llauger, Gabriela
Zavallo, Diego
Blanc, Hervé
Mongelli, Vanesa Claudia
Truol, Graciela Ana Maria
Saleh, María-Carla
Asurmendi, Sebastian
Del Vas, Mariana
author_role author
author2 Dumon, Analia Delina
Mattio, Maria Fernanda
Arguello Caro, Evangelina Beatriz
Llauger, Gabriela
Zavallo, Diego
Blanc, Hervé
Mongelli, Vanesa Claudia
Truol, Graciela Ana Maria
Saleh, María-Carla
Asurmendi, Sebastian
Del Vas, Mariana
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARN
Virus de las Plantas
Trigo
Fulgoroidea
Plant Viruses
Wheat
RNA
Virus del Mal de Río Cuarto
Fijivirus
topic ARN
Virus de las Plantas
Trigo
Fulgoroidea
Plant Viruses
Wheat
RNA
Virus del Mal de Río Cuarto
Fijivirus
dc.description.none.fl_txt_mv Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.
Inst. de Biotecnología
Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dumon, Analia Delina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Blanc, Hervé. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia
Fil: Truol, Graciela Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Mongelli, Vanesa Claudia. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia
Fil: Saleh, María-Carla. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01T18:02:06Z
2017-09-01T18:02:06Z
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1108
http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/full
de Haro LA, Dumón AD, Mattio MF, Argüello Caro EB, Llauger G, Zavallo D, Blanc H, Mongelli VC, Truol G, Saleh M-C, Asurmendi S and del Vas M (2017) Mal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper Vector. Front. Plant Sci. 8:766
https://doi.org/10.3389/fpls.2017.00766
url http://hdl.handle.net/20.500.12123/1108
http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/full
https://doi.org/10.3389/fpls.2017.00766
identifier_str_mv de Haro LA, Dumón AD, Mattio MF, Argüello Caro EB, Llauger G, Zavallo D, Blanc H, Mongelli VC, Truol G, Saleh M-C, Asurmendi S and del Vas M (2017) Mal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper Vector. Front. Plant Sci. 8:766
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers in plant science 8 :766. (May 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.885934

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