A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris

Autores
Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina.We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiledfungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.
Instituto de Patología Vegetal
Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina
Fil: Brücher, Elsa. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina
Fuente
European journal of plant pathology 144 (1) : 217–223. (January 2016)
Materia
Bacillus Subtilis
Control Biológico
Cebolla
Allium Cepa
Organismos Transmitidos por Suelo
Soilborne Organisms
Onions
Biological Control
Setophoma Terrestris
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2596
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2596
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network_name_str INTA Digital (INTA)
spelling A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestrisAlbarracín Orio, Andrea GeorginaBrücher, ElsaDucasse, Daniel AdrianBacillus SubtilisControl BiológicoCebollaAllium CepaOrganismos Transmitidos por SueloSoilborne OrganismsOnionsBiological ControlSetophoma TerrestrisPink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina.We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiledfungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.Instituto de Patología VegetalFil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; ArgentinaFil: Brücher, Elsa. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; ArgentinaFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina2018-06-11T18:29:50Z2018-06-11T18:29:50Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2596https://link.springer.com/article/10.1007/s10658-015-0762-00929-18731573-8469 (Online)https://doi.org/10.1007/s10658-015-0762-0European journal of plant pathology 144 (1) : 217–223. (January 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:12Zoai:localhost:20.500.12123/2596instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:12.704INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
spellingShingle A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
Albarracín Orio, Andrea Georgina
Bacillus Subtilis
Control Biológico
Cebolla
Allium Cepa
Organismos Transmitidos por Suelo
Soilborne Organisms
Onions
Biological Control
Setophoma Terrestris
title_short A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_full A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_fullStr A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_full_unstemmed A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_sort A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
dc.creator.none.fl_str_mv Albarracín Orio, Andrea Georgina
Brücher, Elsa
Ducasse, Daniel Adrian
author Albarracín Orio, Andrea Georgina
author_facet Albarracín Orio, Andrea Georgina
Brücher, Elsa
Ducasse, Daniel Adrian
author_role author
author2 Brücher, Elsa
Ducasse, Daniel Adrian
author2_role author
author
dc.subject.none.fl_str_mv Bacillus Subtilis
Control Biológico
Cebolla
Allium Cepa
Organismos Transmitidos por Suelo
Soilborne Organisms
Onions
Biological Control
Setophoma Terrestris
topic Bacillus Subtilis
Control Biológico
Cebolla
Allium Cepa
Organismos Transmitidos por Suelo
Soilborne Organisms
Onions
Biological Control
Setophoma Terrestris
dc.description.none.fl_txt_mv Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina.We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiledfungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.
Instituto de Patología Vegetal
Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina
Fil: Brücher, Elsa. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina
description Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina.We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiledfungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018-06-11T18:29:50Z
2018-06-11T18:29:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2596
https://link.springer.com/article/10.1007/s10658-015-0762-0
0929-1873
1573-8469 (Online)
https://doi.org/10.1007/s10658-015-0762-0
url http://hdl.handle.net/20.500.12123/2596
https://link.springer.com/article/10.1007/s10658-015-0762-0
https://doi.org/10.1007/s10658-015-0762-0
identifier_str_mv 0929-1873
1573-8469 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv European journal of plant pathology 144 (1) : 217–223. (January 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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