A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris

Autores
Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrián
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina. We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiled fungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.
Fil: Albarracín Orio, Andrea Georgina. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brücher, Elsa. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ducasse, Daniel Adrián. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Materia
Bacillus Subtilis
Biocontrol
Onion
Setophoma Terrestris
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69713
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69713
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestrisAlbarracín Orio, Andrea GeorginaBrücher, ElsaDucasse, Daniel AdriánBacillus SubtilisBiocontrolOnionSetophoma Terrestrishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina. We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiled fungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.Fil: Albarracín Orio, Andrea Georgina. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brücher, Elsa. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ducasse, Daniel Adrián. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaSpringer2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69713Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrián; A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris; Springer; European Journal of Plant Pathology; 144; 1; 1-2016; 217-2230929-18731573-8469CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-015-0762-0info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10658-015-0762-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:24Zoai:ri.conicet.gov.ar:11336/69713instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:24.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
spellingShingle A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
Albarracín Orio, Andrea Georgina
Bacillus Subtilis
Biocontrol
Onion
Setophoma Terrestris
title_short A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_full A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_fullStr A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_full_unstemmed A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
title_sort A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
dc.creator.none.fl_str_mv Albarracín Orio, Andrea Georgina
Brücher, Elsa
Ducasse, Daniel Adrián
author Albarracín Orio, Andrea Georgina
author_facet Albarracín Orio, Andrea Georgina
Brücher, Elsa
Ducasse, Daniel Adrián
author_role author
author2 Brücher, Elsa
Ducasse, Daniel Adrián
author2_role author
author
dc.subject.none.fl_str_mv Bacillus Subtilis
Biocontrol
Onion
Setophoma Terrestris
topic Bacillus Subtilis
Biocontrol
Onion
Setophoma Terrestris
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina. We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiled fungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.
Fil: Albarracín Orio, Andrea Georgina. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brücher, Elsa. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ducasse, Daniel Adrián. Area de Ciencias Agrarias, Ingeniería, Ciencias Biológicas y de la Salud de la Universidad Católica de Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
description Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina. We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiled fungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69713
Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrián; A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris; Springer; European Journal of Plant Pathology; 144; 1; 1-2016; 217-223
0929-1873
1573-8469
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69713
identifier_str_mv Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrián; A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris; Springer; European Journal of Plant Pathology; 144; 1; 1-2016; 217-223
0929-1873
1573-8469
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-015-0762-0
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10658-015-0762-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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