Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina

Autores
Masana, Marcelo Oscar; D’Astek, Beatriz A.; Palladino, Pablo Martin; Galli, Lucia; Del Castillo, Lourdes; Carbonari, Claudia Carolina; Leotta, Gerardo A.; Vilacoba, E.; Irino, K.; Rivas, Marta
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.
Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: D’astek, B.A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Galli, Lucia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Del Castillo, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Carbonari, C. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Leotta, G. A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Vilacoba, E. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Irino, K. Instituto Adolfo Lutz. Secao de Bacteriologia; Brasil.
Fil: Rivas, M. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fuente
Journal of Food Protection 74 (12 ): 2008–2017 (2011)
Materia
Escherichia coli
Carne de Res
Mataderos
Beef
Abattoirs
Genotypes
Genotipos
Genotypic
Non-O157 Shiga Toxin
Beef Abattoirs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5048
Acceder
id INTADig_1dde02fb5c79abfb22b222ec4a0ae8f2
oai_identifier_str oai:localhost:20.500.12123/5048
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of ArgentinaMasana, Marcelo OscarD’Astek, Beatriz A.Palladino, Pablo MartinGalli, LuciaDel Castillo, LourdesCarbonari, Claudia CarolinaLeotta, Gerardo A.Vilacoba, E.Irino, K.Rivas, MartaEscherichia coliCarne de ResMataderosBeefAbattoirsGenotypesGenotiposGenotypicNon-O157 Shiga ToxinBeef AbattoirsThe non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: D’astek, B.A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: Galli, Lucia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Del Castillo, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: Carbonari, C. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Leotta, G. A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Vilacoba, E. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Irino, K. Instituto Adolfo Lutz. Secao de Bacteriologia; Brasil.Fil: Rivas, M. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.2019-05-07T11:27:27Z2019-05-07T11:27:27Z2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5048https://jfoodprotection.org/doi/pdf/10.4315/0362-028X.JFP-11-1890362-028X1944-9097https://doi.org/10.4315/0362-028X.JFP-11-189Journal of Food Protection 74 (12 ): 2008–2017 (2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:57Zoai:localhost:20.500.12123/5048instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:58.104INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
title Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
spellingShingle Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
Masana, Marcelo Oscar
Escherichia coli
Carne de Res
Mataderos
Beef
Abattoirs
Genotypes
Genotipos
Genotypic
Non-O157 Shiga Toxin
Beef Abattoirs
title_short Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
title_full Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
title_fullStr Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
title_full_unstemmed Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
title_sort Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina
dc.creator.none.fl_str_mv Masana, Marcelo Oscar
D’Astek, Beatriz A.
Palladino, Pablo Martin
Galli, Lucia
Del Castillo, Lourdes
Carbonari, Claudia Carolina
Leotta, Gerardo A.
Vilacoba, E.
Irino, K.
Rivas, Marta
author Masana, Marcelo Oscar
author_facet Masana, Marcelo Oscar
D’Astek, Beatriz A.
Palladino, Pablo Martin
Galli, Lucia
Del Castillo, Lourdes
Carbonari, Claudia Carolina
Leotta, Gerardo A.
Vilacoba, E.
Irino, K.
Rivas, Marta
author_role author
author2 D’Astek, Beatriz A.
Palladino, Pablo Martin
Galli, Lucia
Del Castillo, Lourdes
Carbonari, Claudia Carolina
Leotta, Gerardo A.
Vilacoba, E.
Irino, K.
Rivas, Marta
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Carne de Res
Mataderos
Beef
Abattoirs
Genotypes
Genotipos
Genotypic
Non-O157 Shiga Toxin
Beef Abattoirs
topic Escherichia coli
Carne de Res
Mataderos
Beef
Abattoirs
Genotypes
Genotipos
Genotypic
Non-O157 Shiga Toxin
Beef Abattoirs
dc.description.none.fl_txt_mv The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.
Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: D’astek, B.A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Galli, Lucia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Del Castillo, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Carbonari, C. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Leotta, G. A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Vilacoba, E. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Irino, K. Instituto Adolfo Lutz. Secao de Bacteriologia; Brasil.
Fil: Rivas, M. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
description The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
2019-05-07T11:27:27Z
2019-05-07T11:27:27Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5048
https://jfoodprotection.org/doi/pdf/10.4315/0362-028X.JFP-11-189
0362-028X
1944-9097
https://doi.org/10.4315/0362-028X.JFP-11-189
url http://hdl.handle.net/20.500.12123/5048
https://jfoodprotection.org/doi/pdf/10.4315/0362-028X.JFP-11-189
https://doi.org/10.4315/0362-028X.JFP-11-189
identifier_str_mv 0362-028X
1944-9097
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Food Protection 74 (12 ): 2008–2017 (2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341365864726528
score 12.623145

Publicaciones similares