Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle

Autores
Okuno, Kentaro; Awasthi, Sharda Prasad; Kopprio, Germán Adolfo; Iguchi, Atsushi; Hatanaka, Noritoshi; Hinenoya, Atsushi; Lara, Ruben Jose; Yamasaki, Shinji
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1, 58 with stx2, and 19 with both stx1 and stx2. Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1, 34 stx2, and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each) , Og22, Og153, Og157 (3 from each) and others. Various stx2 gene37 subtypes were identified in 42 STEC strains: 13 positive cases for stx2a, 11 for stx2c, 3 for stx2g, 10 for stx2a and stx2d, 4 for stx2a and stx2c, and 1 for stx2b, stx2c and stx2g. efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.
Fil: Okuno, Kentaro. Osaka Prefecture University; Japón
Fil: Awasthi, Sharda Prasad. Osaka Prefecture University; Japón
Fil: Kopprio, Germán Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz - Institute of Freshwater Ecology and Inland Fisheries; Alemania
Fil: Iguchi, Atsushi. University of Miyazaki; Japón
Fil: Hatanaka, Noritoshi. Osaka Prefecture University; Japón
Fil: Hinenoya, Atsushi. Osaka Prefecture University; Japón
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Fil: Yamasaki, Shinji. Osaka Prefecture University; Japón
Materia
ESCHERICHIA COLI
ARGENTINA
BEEF CATTLE
SHIGA TOXIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158534
Acceder
id CONICETDig_32f832014312d218c8ed153f36d507a4
oai_identifier_str oai:ri.conicet.gov.ar:11336/158534
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattleOkuno, KentaroAwasthi, Sharda PrasadKopprio, Germán AdolfoIguchi, AtsushiHatanaka, NoritoshiHinenoya, AtsushiLara, Ruben JoseYamasaki, ShinjiESCHERICHIA COLIARGENTINABEEF CATTLESHIGA TOXINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1, 58 with stx2, and 19 with both stx1 and stx2. Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1, 34 stx2, and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each) , Og22, Og153, Og157 (3 from each) and others. Various stx2 gene37 subtypes were identified in 42 STEC strains: 13 positive cases for stx2a, 11 for stx2c, 3 for stx2g, 10 for stx2a and stx2d, 4 for stx2a and stx2c, and 1 for stx2b, stx2c and stx2g. efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.Fil: Okuno, Kentaro. Osaka Prefecture University; JapónFil: Awasthi, Sharda Prasad. Osaka Prefecture University; JapónFil: Kopprio, Germán Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz - Institute of Freshwater Ecology and Inland Fisheries; AlemaniaFil: Iguchi, Atsushi. University of Miyazaki; JapónFil: Hatanaka, Noritoshi. Osaka Prefecture University; JapónFil: Hinenoya, Atsushi. Osaka Prefecture University; JapónFil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Yamasaki, Shinji. Osaka Prefecture University; JapónJapanese Society of Veterinary Science2021-02-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158534Okuno, Kentaro; Awasthi, Sharda Prasad; Kopprio, Germán Adolfo; Iguchi, Atsushi; Hatanaka, Noritoshi; et al.; Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle; Japanese Society of Veterinary Science; Journal Of Veterinary Medical Science; 83; 4; 22-2-2021; 630-6360916-72501347-7439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_21-0002/_articleinfo:eu-repo/semantics/altIdentifier/doi/10.1292/jvms.21-0002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:13Zoai:ri.conicet.gov.ar:11336/158534instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:13.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
title Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
spellingShingle Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
Okuno, Kentaro
ESCHERICHIA COLI
ARGENTINA
BEEF CATTLE
SHIGA TOXIN
title_short Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
title_full Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
title_fullStr Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
title_full_unstemmed Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
title_sort Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle
dc.creator.none.fl_str_mv Okuno, Kentaro
Awasthi, Sharda Prasad
Kopprio, Germán Adolfo
Iguchi, Atsushi
Hatanaka, Noritoshi
Hinenoya, Atsushi
Lara, Ruben Jose
Yamasaki, Shinji
author Okuno, Kentaro
author_facet Okuno, Kentaro
Awasthi, Sharda Prasad
Kopprio, Germán Adolfo
Iguchi, Atsushi
Hatanaka, Noritoshi
Hinenoya, Atsushi
Lara, Ruben Jose
Yamasaki, Shinji
author_role author
author2 Awasthi, Sharda Prasad
Kopprio, Germán Adolfo
Iguchi, Atsushi
Hatanaka, Noritoshi
Hinenoya, Atsushi
Lara, Ruben Jose
Yamasaki, Shinji
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESCHERICHIA COLI
ARGENTINA
BEEF CATTLE
SHIGA TOXIN
topic ESCHERICHIA COLI
ARGENTINA
BEEF CATTLE
SHIGA TOXIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1, 58 with stx2, and 19 with both stx1 and stx2. Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1, 34 stx2, and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each) , Og22, Og153, Og157 (3 from each) and others. Various stx2 gene37 subtypes were identified in 42 STEC strains: 13 positive cases for stx2a, 11 for stx2c, 3 for stx2g, 10 for stx2a and stx2d, 4 for stx2a and stx2c, and 1 for stx2b, stx2c and stx2g. efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.
Fil: Okuno, Kentaro. Osaka Prefecture University; Japón
Fil: Awasthi, Sharda Prasad. Osaka Prefecture University; Japón
Fil: Kopprio, Germán Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz - Institute of Freshwater Ecology and Inland Fisheries; Alemania
Fil: Iguchi, Atsushi. University of Miyazaki; Japón
Fil: Hatanaka, Noritoshi. Osaka Prefecture University; Japón
Fil: Hinenoya, Atsushi. Osaka Prefecture University; Japón
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Fil: Yamasaki, Shinji. Osaka Prefecture University; Japón
description The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1, 58 with stx2, and 19 with both stx1 and stx2. Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1, 34 stx2, and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each) , Og22, Og153, Og157 (3 from each) and others. Various stx2 gene37 subtypes were identified in 42 STEC strains: 13 positive cases for stx2a, 11 for stx2c, 3 for stx2g, 10 for stx2a and stx2d, 4 for stx2a and stx2c, and 1 for stx2b, stx2c and stx2g. efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158534
Okuno, Kentaro; Awasthi, Sharda Prasad; Kopprio, Germán Adolfo; Iguchi, Atsushi; Hatanaka, Noritoshi; et al.; Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle; Japanese Society of Veterinary Science; Journal Of Veterinary Medical Science; 83; 4; 22-2-2021; 630-636
0916-7250
1347-7439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158534
identifier_str_mv Okuno, Kentaro; Awasthi, Sharda Prasad; Kopprio, Germán Adolfo; Iguchi, Atsushi; Hatanaka, Noritoshi; et al.; Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle; Japanese Society of Veterinary Science; Journal Of Veterinary Medical Science; 83; 4; 22-2-2021; 630-636
0916-7250
1347-7439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_21-0002/_article
info:eu-repo/semantics/altIdentifier/doi/10.1292/jvms.21-0002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Japanese Society of Veterinary Science
publisher.none.fl_str_mv Japanese Society of Veterinary Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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