Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle

Autores
Gonzalez, Magali Lucia Rosa; Valero, Eva; Chimeno, Selva Valeria; Garrido Fernandez, Antonio; Rodriguez Gomez, Francisco; Rojo, Cecilia; Paolinelli, Marcos; Arroyo Lopez, Francisco Noe; Combina, Mariana; Mercado, Laura Analia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle.
EEA Mendoza
Fil: Gonzalez, Magali Lucia Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Magali Lucia Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Valero, Eva. Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica; España
Fil: Chimeno, Selva Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garrido Fernandez, Antonio. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España
Fil: Rodriguez Gomez, Francisco. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España
Fil: Rojo, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Rojo, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Arroyo Lopez, Francisco Noe. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Mercado, Laura Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fuente
LWT, Food Science and Technology 158 : 113157 (2022)
Materia
Vid
Levadura
Identificación
Microsatélites
Saccharomyces cerevisiae
Grapevines
Yeasts
Identification
Microsatellites
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11335
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11335
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycleGonzalez, Magali Lucia RosaValero, EvaChimeno, Selva ValeriaGarrido Fernandez, AntonioRodriguez Gomez, FranciscoRojo, CeciliaPaolinelli, MarcosArroyo Lopez, Francisco NoeCombina, MarianaMercado, Laura AnaliaVidLevaduraIdentificaciónMicrosatélitesSaccharomyces cerevisiaeGrapevinesYeastsIdentificationMicrosatellitesSaccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle.EEA MendozaFil: Gonzalez, Magali Lucia Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Magali Lucia Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Valero, Eva. Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica; EspañaFil: Chimeno, Selva Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garrido Fernandez, Antonio. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); EspañaFil: Rodriguez Gomez, Francisco. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); EspañaFil: Rojo, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Rojo, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Arroyo Lopez, Francisco Noe. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); EspañaFil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Mercado, Laura Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaElsevier2022-03-08T16:25:17Z2022-03-08T16:25:17Z2022-03-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11335https://www.sciencedirect.com/science/article/pii/S00236438220009250023-6438https://doi.org/10.1016/j.lwt.2022.113157LWT, Food Science and Technology 158 : 113157 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:17Zoai:localhost:20.500.12123/11335instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:17.691INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
title Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
spellingShingle Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
Gonzalez, Magali Lucia Rosa
Vid
Levadura
Identificación
Microsatélites
Saccharomyces cerevisiae
Grapevines
Yeasts
Identification
Microsatellites
title_short Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
title_full Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
title_fullStr Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
title_full_unstemmed Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
title_sort Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
dc.creator.none.fl_str_mv Gonzalez, Magali Lucia Rosa
Valero, Eva
Chimeno, Selva Valeria
Garrido Fernandez, Antonio
Rodriguez Gomez, Francisco
Rojo, Cecilia
Paolinelli, Marcos
Arroyo Lopez, Francisco Noe
Combina, Mariana
Mercado, Laura Analia
author Gonzalez, Magali Lucia Rosa
author_facet Gonzalez, Magali Lucia Rosa
Valero, Eva
Chimeno, Selva Valeria
Garrido Fernandez, Antonio
Rodriguez Gomez, Francisco
Rojo, Cecilia
Paolinelli, Marcos
Arroyo Lopez, Francisco Noe
Combina, Mariana
Mercado, Laura Analia
author_role author
author2 Valero, Eva
Chimeno, Selva Valeria
Garrido Fernandez, Antonio
Rodriguez Gomez, Francisco
Rojo, Cecilia
Paolinelli, Marcos
Arroyo Lopez, Francisco Noe
Combina, Mariana
Mercado, Laura Analia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Vid
Levadura
Identificación
Microsatélites
Saccharomyces cerevisiae
Grapevines
Yeasts
Identification
Microsatellites
topic Vid
Levadura
Identificación
Microsatélites
Saccharomyces cerevisiae
Grapevines
Yeasts
Identification
Microsatellites
dc.description.none.fl_txt_mv Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle.
EEA Mendoza
Fil: Gonzalez, Magali Lucia Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Magali Lucia Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Valero, Eva. Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica; España
Fil: Chimeno, Selva Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garrido Fernandez, Antonio. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España
Fil: Rodriguez Gomez, Francisco. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España
Fil: Rojo, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Rojo, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Arroyo Lopez, Francisco Noe. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Mercado, Laura Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
description Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-08T16:25:17Z
2022-03-08T16:25:17Z
2022-03-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11335
https://www.sciencedirect.com/science/article/pii/S0023643822000925
0023-6438
https://doi.org/10.1016/j.lwt.2022.113157
url http://hdl.handle.net/20.500.12123/11335
https://www.sciencedirect.com/science/article/pii/S0023643822000925
https://doi.org/10.1016/j.lwt.2022.113157
identifier_str_mv 0023-6438
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv LWT, Food Science and Technology 158 : 113157 (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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