Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina

Autores
Berruezo, Lorena Andrea; Mercado Cardenas, Guadalupe Eugenia; Harries, Eleonora Del Milagro; Stenglein, Sebastian Alberto; Curti, Ramiro Nestor; Rodriguero, Marcela Silvina; Galvan, Marta Zulema
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production.
EEA Salta
Fil: Berruezo, Lorena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Harries, Eleonora Del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Curti, Ramiro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fuente
European Journal of Plant Pathology 151 (4) : 1065–1079 (August 2018)
Materia
Tabaco
Nicotiana tabacum
Enfermedades de las Plantas
Fusarium
Patogenicidad
Podredumbre de la Raíz
Marchitez
Tobacco
Plant Diseases
Pathogenicity
Root Rots
Wilts
Tabaco (planta)
Región Noroeste, Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3385
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3385
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Characterization of Fusarium species associated with tobacco diseases in Northwestern ArgentinaBerruezo, Lorena AndreaMercado Cardenas, Guadalupe EugeniaHarries, Eleonora Del MilagroStenglein, Sebastian AlbertoCurti, Ramiro NestorRodriguero, Marcela SilvinaGalvan, Marta ZulemaTabacoNicotiana tabacumEnfermedades de las PlantasFusariumPatogenicidadPodredumbre de la RaízMarchitezTobaccoPlant DiseasesPathogenicityRoot RotsWiltsTabaco (planta)Región Noroeste, ArgentinaTobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production.EEA SaltaFil: Berruezo, Lorena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Harries, Eleonora Del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Curti, Ramiro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina2018-09-18T13:39:58Z2018-09-18T13:39:58Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3385https://link.springer.com/article/10.1007/s10658-018-1443-60929-18731573-8469https://doi.org/10.1007/s10658-018-1443-6European Journal of Plant Pathology 151 (4) : 1065–1079 (August 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-11T10:22:45Zoai:localhost:20.500.12123/3385instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:46.06INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
spellingShingle Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
Berruezo, Lorena Andrea
Tabaco
Nicotiana tabacum
Enfermedades de las Plantas
Fusarium
Patogenicidad
Podredumbre de la Raíz
Marchitez
Tobacco
Plant Diseases
Pathogenicity
Root Rots
Wilts
Tabaco (planta)
Región Noroeste, Argentina
title_short Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_full Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_fullStr Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_full_unstemmed Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_sort Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
dc.creator.none.fl_str_mv Berruezo, Lorena Andrea
Mercado Cardenas, Guadalupe Eugenia
Harries, Eleonora Del Milagro
Stenglein, Sebastian Alberto
Curti, Ramiro Nestor
Rodriguero, Marcela Silvina
Galvan, Marta Zulema
author Berruezo, Lorena Andrea
author_facet Berruezo, Lorena Andrea
Mercado Cardenas, Guadalupe Eugenia
Harries, Eleonora Del Milagro
Stenglein, Sebastian Alberto
Curti, Ramiro Nestor
Rodriguero, Marcela Silvina
Galvan, Marta Zulema
author_role author
author2 Mercado Cardenas, Guadalupe Eugenia
Harries, Eleonora Del Milagro
Stenglein, Sebastian Alberto
Curti, Ramiro Nestor
Rodriguero, Marcela Silvina
Galvan, Marta Zulema
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Tabaco
Nicotiana tabacum
Enfermedades de las Plantas
Fusarium
Patogenicidad
Podredumbre de la Raíz
Marchitez
Tobacco
Plant Diseases
Pathogenicity
Root Rots
Wilts
Tabaco (planta)
Región Noroeste, Argentina
topic Tabaco
Nicotiana tabacum
Enfermedades de las Plantas
Fusarium
Patogenicidad
Podredumbre de la Raíz
Marchitez
Tobacco
Plant Diseases
Pathogenicity
Root Rots
Wilts
Tabaco (planta)
Región Noroeste, Argentina
dc.description.none.fl_txt_mv Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production.
EEA Salta
Fil: Berruezo, Lorena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Harries, Eleonora Del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Curti, Ramiro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
description Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-18T13:39:58Z
2018-09-18T13:39:58Z
2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3385
https://link.springer.com/article/10.1007/s10658-018-1443-6
0929-1873
1573-8469
https://doi.org/10.1007/s10658-018-1443-6
url http://hdl.handle.net/20.500.12123/3385
https://link.springer.com/article/10.1007/s10658-018-1443-6
https://doi.org/10.1007/s10658-018-1443-6
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv European Journal of Plant Pathology 151 (4) : 1065–1079 (August 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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