Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Autores
Mercado Cardenas, Guadalupe Eugenia; Galvan, Marta Zulema; Barrera, Viviana Andrea; Rodriguero, Marcela Silvina; Carmona, Marcelo Anibal; March, Guillermo Juan; Ramallo, Ana Carolina; Shew, H. David
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.
EEA Salta
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina.
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: March, Guillermo Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Ramallo, Ana C.. Universidad Nacional de Tucumán; Argentina
Fil: Shew, H. David. University of North Carolina. Department of Plant Pathology; Estados Unidos
Fuente
Tropical Plant Pathology 40 (3) : 160–168 (June 2015)
Materia
Rhizoctonia solani
Nicotiana tabacum
Tabaco
Enfermedades de las Plantas
Rendimiento
Calidad
Tobacco
Plant Diseases
Yields
Quality
Región Noroeste, Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3622
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3622
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern ArgentinaMercado Cardenas, Guadalupe EugeniaGalvan, Marta ZulemaBarrera, Viviana AndreaRodriguero, Marcela SilvinaCarmona, Marcelo AnibalMarch, Guillermo JuanRamallo, Ana CarolinaShew, H. DavidRhizoctonia solaniNicotiana tabacumTabacoEnfermedades de las PlantasRendimientoCalidadTobaccoPlant DiseasesYieldsQualityRegión Noroeste, ArgentinaIn Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.EEA SaltaFil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina.Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: March, Guillermo Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Ramallo, Ana C.. Universidad Nacional de Tucumán; ArgentinaFil: Shew, H. David. University of North Carolina. Department of Plant Pathology; Estados Unidos2018-10-18T12:38:37Z2018-10-18T12:38:37Z2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s40858-015-0035-7http://hdl.handle.net/20.500.12123/36221983-2052https://doi.org/10.1007/s40858-015-0035-7Tropical Plant Pathology 40 (3) : 160–168 (June 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-11T10:22:47Zoai:localhost:20.500.12123/3622instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:48.158INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
spellingShingle Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
Mercado Cardenas, Guadalupe Eugenia
Rhizoctonia solani
Nicotiana tabacum
Tabaco
Enfermedades de las Plantas
Rendimiento
Calidad
Tobacco
Plant Diseases
Yields
Quality
Región Noroeste, Argentina
title_short Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_full Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_fullStr Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_full_unstemmed Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_sort Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
dc.creator.none.fl_str_mv Mercado Cardenas, Guadalupe Eugenia
Galvan, Marta Zulema
Barrera, Viviana Andrea
Rodriguero, Marcela Silvina
Carmona, Marcelo Anibal
March, Guillermo Juan
Ramallo, Ana Carolina
Shew, H. David
author Mercado Cardenas, Guadalupe Eugenia
author_facet Mercado Cardenas, Guadalupe Eugenia
Galvan, Marta Zulema
Barrera, Viviana Andrea
Rodriguero, Marcela Silvina
Carmona, Marcelo Anibal
March, Guillermo Juan
Ramallo, Ana Carolina
Shew, H. David
author_role author
author2 Galvan, Marta Zulema
Barrera, Viviana Andrea
Rodriguero, Marcela Silvina
Carmona, Marcelo Anibal
March, Guillermo Juan
Ramallo, Ana Carolina
Shew, H. David
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rhizoctonia solani
Nicotiana tabacum
Tabaco
Enfermedades de las Plantas
Rendimiento
Calidad
Tobacco
Plant Diseases
Yields
Quality
Región Noroeste, Argentina
topic Rhizoctonia solani
Nicotiana tabacum
Tabaco
Enfermedades de las Plantas
Rendimiento
Calidad
Tobacco
Plant Diseases
Yields
Quality
Región Noroeste, Argentina
dc.description.none.fl_txt_mv In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.
EEA Salta
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina.
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: March, Guillermo Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Ramallo, Ana C.. Universidad Nacional de Tucumán; Argentina
Fil: Shew, H. David. University of North Carolina. Department of Plant Pathology; Estados Unidos
description In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
2018-10-18T12:38:37Z
2018-10-18T12:38:37Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s40858-015-0035-7
http://hdl.handle.net/20.500.12123/3622
1983-2052
https://doi.org/10.1007/s40858-015-0035-7
url https://link.springer.com/article/10.1007/s40858-015-0035-7
http://hdl.handle.net/20.500.12123/3622
https://doi.org/10.1007/s40858-015-0035-7
identifier_str_mv 1983-2052
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Tropical Plant Pathology 40 (3) : 160–168 (June 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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