MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina

Autores
Fontana, Paola Daniela; Tomasini, Nicolás; Fontana, Cecilia Alejandra; Di Pauli, Valentina; Cocconcelli, Pier Sandro; Vignolo, Graciela Margarita; Salazar, Sergio Miguel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Acidovorax species cause a wide range of economically important diseases in monocotyledonous and dicotyledonous plants, including sugarcane, corn, rice, oats, millet, foxtail watermelon and orchids. In Argentina, the red stripe disease of sugarcane caused by A. avenae affects 30% of the milling stems with important economic losses. To explore the genetic diversity of this bacterium associated with red stripe in Argentina, MLST was applied. This study included 15 local strains isolated from four different sugarcane planting regions and selected after RAPD analysis and reference strains of A. citrulli, A. avenae, and A. oryzae to investigate their phylogenetic relationships. MLST analysis resulted in five sequence types (STs) among the sugarcane A. avenae strains which constitute a clonal complex, meaning a common and close origin. Sugarcane strains were related to A. avenae from other hosts and distant to A. citrulli. Signals of frequent recombination in several lineages of A. avenae was detected and we observed that A. oryzae is closely related to A. avenae strains. This study provides valuable data in the field of epidemiological and evolutionary investigations of novel clone of A. avenae strains causing sugarcane red stripe. The knowledge of the genetic diversity and the specificity strain-host are important to select the genotypes with the best response to the red stripe disease.
EEA Famaillá
Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile ; Italia
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fuente
Phytopathology 109 (3) : 358-365 (March 2019)
Materia
Caña de Azúcar
Enfermedades de las Plantas
Secuencia Nucleotídica
Genética
Genotipos
Sugarcane
Plant Diseases
Nucleotide Sequence
Genetics
Genotypes
Acidovorax avenae
Estría Roja de la Caña de Azúcar
MLST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4207
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4207
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from ArgentinaFontana, Paola DanielaTomasini, NicolásFontana, Cecilia AlejandraDi Pauli, ValentinaCocconcelli, Pier SandroVignolo, Graciela MargaritaSalazar, Sergio MiguelCaña de AzúcarEnfermedades de las PlantasSecuencia NucleotídicaGenéticaGenotiposSugarcanePlant DiseasesNucleotide SequenceGeneticsGenotypesAcidovorax avenaeEstría Roja de la Caña de AzúcarMLSTAcidovorax species cause a wide range of economically important diseases in monocotyledonous and dicotyledonous plants, including sugarcane, corn, rice, oats, millet, foxtail watermelon and orchids. In Argentina, the red stripe disease of sugarcane caused by A. avenae affects 30% of the milling stems with important economic losses. To explore the genetic diversity of this bacterium associated with red stripe in Argentina, MLST was applied. This study included 15 local strains isolated from four different sugarcane planting regions and selected after RAPD analysis and reference strains of A. citrulli, A. avenae, and A. oryzae to investigate their phylogenetic relationships. MLST analysis resulted in five sequence types (STs) among the sugarcane A. avenae strains which constitute a clonal complex, meaning a common and close origin. Sugarcane strains were related to A. avenae from other hosts and distant to A. citrulli. Signals of frequent recombination in several lineages of A. avenae was detected and we observed that A. oryzae is closely related to A. avenae strains. This study provides valuable data in the field of epidemiological and evolutionary investigations of novel clone of A. avenae strains causing sugarcane red stripe. The knowledge of the genetic diversity and the specificity strain-host are important to select the genotypes with the best response to the red stripe disease.EEA FamailláFil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile ; ItaliaFil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; ArgentinaFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaAmerican Phytopathological Society2019-01-04T11:51:51Z2019-01-04T11:51:51Z2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4207https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-18-0303-R0031-949X1943-7684https://doi.org/10.1094/PHYTO-08-18-0303-RPhytopathology 109 (3) : 358-365 (March 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:44Zoai:localhost:20.500.12123/4207instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:45.802INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
title MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
spellingShingle MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
Fontana, Paola Daniela
Caña de Azúcar
Enfermedades de las Plantas
Secuencia Nucleotídica
Genética
Genotipos
Sugarcane
Plant Diseases
Nucleotide Sequence
Genetics
Genotypes
Acidovorax avenae
Estría Roja de la Caña de Azúcar
MLST
title_short MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
title_full MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
title_fullStr MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
title_full_unstemmed MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
title_sort MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina
dc.creator.none.fl_str_mv Fontana, Paola Daniela
Tomasini, Nicolás
Fontana, Cecilia Alejandra
Di Pauli, Valentina
Cocconcelli, Pier Sandro
Vignolo, Graciela Margarita
Salazar, Sergio Miguel
author Fontana, Paola Daniela
author_facet Fontana, Paola Daniela
Tomasini, Nicolás
Fontana, Cecilia Alejandra
Di Pauli, Valentina
Cocconcelli, Pier Sandro
Vignolo, Graciela Margarita
Salazar, Sergio Miguel
author_role author
author2 Tomasini, Nicolás
Fontana, Cecilia Alejandra
Di Pauli, Valentina
Cocconcelli, Pier Sandro
Vignolo, Graciela Margarita
Salazar, Sergio Miguel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Caña de Azúcar
Enfermedades de las Plantas
Secuencia Nucleotídica
Genética
Genotipos
Sugarcane
Plant Diseases
Nucleotide Sequence
Genetics
Genotypes
Acidovorax avenae
Estría Roja de la Caña de Azúcar
MLST
topic Caña de Azúcar
Enfermedades de las Plantas
Secuencia Nucleotídica
Genética
Genotipos
Sugarcane
Plant Diseases
Nucleotide Sequence
Genetics
Genotypes
Acidovorax avenae
Estría Roja de la Caña de Azúcar
MLST
dc.description.none.fl_txt_mv Acidovorax species cause a wide range of economically important diseases in monocotyledonous and dicotyledonous plants, including sugarcane, corn, rice, oats, millet, foxtail watermelon and orchids. In Argentina, the red stripe disease of sugarcane caused by A. avenae affects 30% of the milling stems with important economic losses. To explore the genetic diversity of this bacterium associated with red stripe in Argentina, MLST was applied. This study included 15 local strains isolated from four different sugarcane planting regions and selected after RAPD analysis and reference strains of A. citrulli, A. avenae, and A. oryzae to investigate their phylogenetic relationships. MLST analysis resulted in five sequence types (STs) among the sugarcane A. avenae strains which constitute a clonal complex, meaning a common and close origin. Sugarcane strains were related to A. avenae from other hosts and distant to A. citrulli. Signals of frequent recombination in several lineages of A. avenae was detected and we observed that A. oryzae is closely related to A. avenae strains. This study provides valuable data in the field of epidemiological and evolutionary investigations of novel clone of A. avenae strains causing sugarcane red stripe. The knowledge of the genetic diversity and the specificity strain-host are important to select the genotypes with the best response to the red stripe disease.
EEA Famaillá
Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile ; Italia
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
description Acidovorax species cause a wide range of economically important diseases in monocotyledonous and dicotyledonous plants, including sugarcane, corn, rice, oats, millet, foxtail watermelon and orchids. In Argentina, the red stripe disease of sugarcane caused by A. avenae affects 30% of the milling stems with important economic losses. To explore the genetic diversity of this bacterium associated with red stripe in Argentina, MLST was applied. This study included 15 local strains isolated from four different sugarcane planting regions and selected after RAPD analysis and reference strains of A. citrulli, A. avenae, and A. oryzae to investigate their phylogenetic relationships. MLST analysis resulted in five sequence types (STs) among the sugarcane A. avenae strains which constitute a clonal complex, meaning a common and close origin. Sugarcane strains were related to A. avenae from other hosts and distant to A. citrulli. Signals of frequent recombination in several lineages of A. avenae was detected and we observed that A. oryzae is closely related to A. avenae strains. This study provides valuable data in the field of epidemiological and evolutionary investigations of novel clone of A. avenae strains causing sugarcane red stripe. The knowledge of the genetic diversity and the specificity strain-host are important to select the genotypes with the best response to the red stripe disease.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
2019-01-04T11:51:51Z
2019-01-04T11:51:51Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4207
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-18-0303-R
0031-949X
1943-7684
https://doi.org/10.1094/PHYTO-08-18-0303-R
url http://hdl.handle.net/20.500.12123/4207
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-18-0303-R
https://doi.org/10.1094/PHYTO-08-18-0303-R
identifier_str_mv 0031-949X
1943-7684
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Phytopathology 109 (3) : 358-365 (March 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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