Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina

Autores
Fontana, Paola Daniela; Rago, Alejandro Mario; Fontana, Cecilia Alejandra; Vignolo, Graciela Margarita; Cocconcelli, Pier Sandro; Mariotti Martinez, Jorge Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones.
EEA Famaillá
Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fontana, Cecilia Alejandra. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); Italia
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; Argentina
Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); Italia
Fil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fuente
European Journal of Plant Pathology 137 (3) : 525–534 (November 2013)
Materia
Caña de Azúcar
Enfermedades de las Plantas
Variación Genética
Aislamiento
PCR
Sugarcane
Plant Diseases
Genetic Variation
Isolation
Acidovorax avenae
Región Noroeste, Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3102
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3102
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network_name_str INTA Digital (INTA)
spelling Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern ArgentinaFontana, Paola DanielaRago, Alejandro MarioFontana, Cecilia AlejandraVignolo, Graciela MargaritaCocconcelli, Pier SandroMariotti Martinez, Jorge AlbertoCaña de AzúcarEnfermedades de las PlantasVariación GenéticaAislamientoPCRSugarcanePlant DiseasesGenetic VariationIsolationAcidovorax avenaeRegión Noroeste, ArgentinaRed stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones.EEA FamailláFil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fontana, Cecilia Alejandra. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); ItaliaFil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; ArgentinaFil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); ItaliaFil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina2018-08-16T13:47:40Z2018-08-16T13:47:40Z2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s10658-013-0263-yhttp://hdl.handle.net/20.500.12123/31020929-18731573-8469https://doi.org/10.1007/s10658-013-0263-yEuropean Journal of Plant Pathology 137 (3) : 525–534 (November 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:25Zoai:localhost:20.500.12123/3102instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:25.629INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
title Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
spellingShingle Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
Fontana, Paola Daniela
Caña de Azúcar
Enfermedades de las Plantas
Variación Genética
Aislamiento
PCR
Sugarcane
Plant Diseases
Genetic Variation
Isolation
Acidovorax avenae
Región Noroeste, Argentina
title_short Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
title_full Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
title_fullStr Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
title_full_unstemmed Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
title_sort Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
dc.creator.none.fl_str_mv Fontana, Paola Daniela
Rago, Alejandro Mario
Fontana, Cecilia Alejandra
Vignolo, Graciela Margarita
Cocconcelli, Pier Sandro
Mariotti Martinez, Jorge Alberto
author Fontana, Paola Daniela
author_facet Fontana, Paola Daniela
Rago, Alejandro Mario
Fontana, Cecilia Alejandra
Vignolo, Graciela Margarita
Cocconcelli, Pier Sandro
Mariotti Martinez, Jorge Alberto
author_role author
author2 Rago, Alejandro Mario
Fontana, Cecilia Alejandra
Vignolo, Graciela Margarita
Cocconcelli, Pier Sandro
Mariotti Martinez, Jorge Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Caña de Azúcar
Enfermedades de las Plantas
Variación Genética
Aislamiento
PCR
Sugarcane
Plant Diseases
Genetic Variation
Isolation
Acidovorax avenae
Región Noroeste, Argentina
topic Caña de Azúcar
Enfermedades de las Plantas
Variación Genética
Aislamiento
PCR
Sugarcane
Plant Diseases
Genetic Variation
Isolation
Acidovorax avenae
Región Noroeste, Argentina
dc.description.none.fl_txt_mv Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones.
EEA Famaillá
Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fontana, Cecilia Alejandra. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); Italia
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; Argentina
Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); Italia
Fil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
description Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
2018-08-16T13:47:40Z
2018-08-16T13:47:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s10658-013-0263-y
http://hdl.handle.net/20.500.12123/3102
0929-1873
1573-8469
https://doi.org/10.1007/s10658-013-0263-y
url https://link.springer.com/article/10.1007/s10658-013-0263-y
http://hdl.handle.net/20.500.12123/3102
https://doi.org/10.1007/s10658-013-0263-y
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv European Journal of Plant Pathology 137 (3) : 525–534 (November 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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