Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
- Autores
- Bacanelli, Gisele; Olarte, Larissa C.; Silva, Marcio Roberto; Rodrigues, Rudielle A.; Carneiro, Paulo A. M.; Kannene, John B.; Pasquatti, Taynara N.; Takatani, Haruo; Zumarraga, Martin Jose; Etges, Rodrigo N.; Araujo, Flabio Ribeiro de; Verbisck, Newton V.
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
Instituto de Biotecnología
Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil
Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil
Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil
Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil
Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil
Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil
Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil - Fuente
- The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)
- Materia
-
Tuberculosis
Infeccion por Mycobacterium bovis
Tratamiento Ionizante
Desorción
Espectrometría
Infeccion Mycobacterium tuberculosis
Bovina
Mycobacterium bovis Infections
Ionization Treatment
Desorption
Spectrometry
Mycobacterium tuberculosis Infections
Bovinae - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/6567
Ver los metadatos del registro completo
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Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinaeBacanelli, GiseleOlarte, Larissa C.Silva, Marcio RobertoRodrigues, Rudielle A.Carneiro, Paulo A. M.Kannene, John B.Pasquatti, Taynara N.Takatani, HaruoZumarraga, Martin JoseEtges, Rodrigo N.Araujo, Flabio Ribeiro deVerbisck, Newton V.TuberculosisInfeccion por Mycobacterium bovisTratamiento IonizanteDesorciónEspectrometríaInfeccion Mycobacterium tuberculosisBovinaMycobacterium bovis InfectionsIonization TreatmentDesorptionSpectrometryMycobacterium tuberculosis InfectionsBovinaeIn this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.Instituto de BiotecnologíaFil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; BrasilFil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; BrasilFil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; BrasilFil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; BrasilFil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados UnidosFil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados UnidosFil: Pasquatti, Taynara N. Dom Bosco Catholic University; BrasilFil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; BrasilFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; BrasilFil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; BrasilFil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; BrasilThe Japanese Society of Veterinary Science2019-12-26T10:52:17Z2019-12-26T10:52:17Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_articlehttp://hdl.handle.net/20.500.12123/65671347-7439https://doi.org/10.1292/jvms.19-0214The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:51Zoai:localhost:20.500.12123/6567instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:51.614INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
title |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
spellingShingle |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae Bacanelli, Gisele Tuberculosis Infeccion por Mycobacterium bovis Tratamiento Ionizante Desorción Espectrometría Infeccion Mycobacterium tuberculosis Bovina Mycobacterium bovis Infections Ionization Treatment Desorption Spectrometry Mycobacterium tuberculosis Infections Bovinae |
title_short |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
title_full |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
title_fullStr |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
title_full_unstemmed |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
title_sort |
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
dc.creator.none.fl_str_mv |
Bacanelli, Gisele Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. |
author |
Bacanelli, Gisele |
author_facet |
Bacanelli, Gisele Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. |
author_role |
author |
author2 |
Olarte, Larissa C. Silva, Marcio Roberto Rodrigues, Rudielle A. Carneiro, Paulo A. M. Kannene, John B. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Etges, Rodrigo N. Araujo, Flabio Ribeiro de Verbisck, Newton V. |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Tuberculosis Infeccion por Mycobacterium bovis Tratamiento Ionizante Desorción Espectrometría Infeccion Mycobacterium tuberculosis Bovina Mycobacterium bovis Infections Ionization Treatment Desorption Spectrometry Mycobacterium tuberculosis Infections Bovinae |
topic |
Tuberculosis Infeccion por Mycobacterium bovis Tratamiento Ionizante Desorción Espectrometría Infeccion Mycobacterium tuberculosis Bovina Mycobacterium bovis Infections Ionization Treatment Desorption Spectrometry Mycobacterium tuberculosis Infections Bovinae |
dc.description.none.fl_txt_mv |
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. Instituto de Biotecnología Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil |
description |
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-26T10:52:17Z 2019-12-26T10:52:17Z 2019-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article http://hdl.handle.net/20.500.12123/6567 1347-7439 https://doi.org/10.1292/jvms.19-0214 |
url |
https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article http://hdl.handle.net/20.500.12123/6567 https://doi.org/10.1292/jvms.19-0214 |
identifier_str_mv |
1347-7439 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The Japanese Society of Veterinary Science |
publisher.none.fl_str_mv |
The Japanese Society of Veterinary Science |
dc.source.none.fl_str_mv |
The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619140880400384 |
score |
12.559606 |