Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae

Autores
Bacanelli, Gisele; Olarte, Larissa C.; Silva, Marcio Roberto; Rodrigues, Rudielle A.; Carneiro, Paulo A. M.; Kannene, John B.; Pasquatti, Taynara N.; Takatani, Haruo; Zumarraga, Martin Jose; Etges, Rodrigo N.; Araujo, Flabio Ribeiro de; Verbisck, Newton V.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
Instituto de Biotecnología
Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil
Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil
Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil
Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil
Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil
Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil
Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
Fuente
The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)
Materia
Tuberculosis
Infeccion por Mycobacterium bovis
Tratamiento Ionizante
Desorción
Espectrometría
Infeccion Mycobacterium tuberculosis
Bovina
Mycobacterium bovis Infections
Ionization Treatment
Desorption
Spectrometry
Mycobacterium tuberculosis Infections
Bovinae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6567

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oai_identifier_str oai:localhost:20.500.12123/6567
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network_name_str INTA Digital (INTA)
spelling Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinaeBacanelli, GiseleOlarte, Larissa C.Silva, Marcio RobertoRodrigues, Rudielle A.Carneiro, Paulo A. M.Kannene, John B.Pasquatti, Taynara N.Takatani, HaruoZumarraga, Martin JoseEtges, Rodrigo N.Araujo, Flabio Ribeiro deVerbisck, Newton V.TuberculosisInfeccion por Mycobacterium bovisTratamiento IonizanteDesorciónEspectrometríaInfeccion Mycobacterium tuberculosisBovinaMycobacterium bovis InfectionsIonization TreatmentDesorptionSpectrometryMycobacterium tuberculosis InfectionsBovinaeIn this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.Instituto de BiotecnologíaFil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; BrasilFil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; BrasilFil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; BrasilFil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; BrasilFil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados UnidosFil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados UnidosFil: Pasquatti, Taynara N. Dom Bosco Catholic University; BrasilFil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; BrasilFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; BrasilFil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; BrasilFil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; BrasilThe Japanese Society of Veterinary Science2019-12-26T10:52:17Z2019-12-26T10:52:17Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_articlehttp://hdl.handle.net/20.500.12123/65671347-7439https://doi.org/10.1292/jvms.19-0214The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:51Zoai:localhost:20.500.12123/6567instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:51.614INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
title Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
spellingShingle Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
Bacanelli, Gisele
Tuberculosis
Infeccion por Mycobacterium bovis
Tratamiento Ionizante
Desorción
Espectrometría
Infeccion Mycobacterium tuberculosis
Bovina
Mycobacterium bovis Infections
Ionization Treatment
Desorption
Spectrometry
Mycobacterium tuberculosis Infections
Bovinae
title_short Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
title_full Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
title_fullStr Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
title_full_unstemmed Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
title_sort Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
dc.creator.none.fl_str_mv Bacanelli, Gisele
Olarte, Larissa C.
Silva, Marcio Roberto
Rodrigues, Rudielle A.
Carneiro, Paulo A. M.
Kannene, John B.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Etges, Rodrigo N.
Araujo, Flabio Ribeiro de
Verbisck, Newton V.
author Bacanelli, Gisele
author_facet Bacanelli, Gisele
Olarte, Larissa C.
Silva, Marcio Roberto
Rodrigues, Rudielle A.
Carneiro, Paulo A. M.
Kannene, John B.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Etges, Rodrigo N.
Araujo, Flabio Ribeiro de
Verbisck, Newton V.
author_role author
author2 Olarte, Larissa C.
Silva, Marcio Roberto
Rodrigues, Rudielle A.
Carneiro, Paulo A. M.
Kannene, John B.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Etges, Rodrigo N.
Araujo, Flabio Ribeiro de
Verbisck, Newton V.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tuberculosis
Infeccion por Mycobacterium bovis
Tratamiento Ionizante
Desorción
Espectrometría
Infeccion Mycobacterium tuberculosis
Bovina
Mycobacterium bovis Infections
Ionization Treatment
Desorption
Spectrometry
Mycobacterium tuberculosis Infections
Bovinae
topic Tuberculosis
Infeccion por Mycobacterium bovis
Tratamiento Ionizante
Desorción
Espectrometría
Infeccion Mycobacterium tuberculosis
Bovina
Mycobacterium bovis Infections
Ionization Treatment
Desorption
Spectrometry
Mycobacterium tuberculosis Infections
Bovinae
dc.description.none.fl_txt_mv In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
Instituto de Biotecnología
Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil
Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil
Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil
Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil
Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil
Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil
Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
description In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-26T10:52:17Z
2019-12-26T10:52:17Z
2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article
http://hdl.handle.net/20.500.12123/6567
1347-7439
https://doi.org/10.1292/jvms.19-0214
url https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article
http://hdl.handle.net/20.500.12123/6567
https://doi.org/10.1292/jvms.19-0214
identifier_str_mv 1347-7439
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Japanese Society of Veterinary Science
publisher.none.fl_str_mv The Japanese Society of Veterinary Science
dc.source.none.fl_str_mv The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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