The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato

Autores
Mori, Kentaro; Lemaire-Chamley, Martine; Jorly, Joana; Carrari, Fernando; Conte, Mariana; Asamizu, Erika; Mizoguchi, Tsuyoshi; Ezura, Hiroshi; Rothan, Christophe
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling.
Instituto de Biotecnología
Fil: Mori, Kentaro. Institut National de la Recherche Agronomique; Francia
Fil: Lemaire-Chamley, Martine. Institut National de la Recherche Agronomique; Francia
Fil: Jorly, Joana. Institut National de la Recherche Agronomique; Francia
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Conte, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asamizu, Erika. Ryukoku University. Faculty of Agriculture. Department of Plant Life Science; Japón
Fil: Mizoguchi, Tsuyoshi. International Christian University. Department of Natural Sciences; Japón
Fil: Ezura, Hiroshi. University of Tsukuba. Tsukuba Plant Innovation Research Center. Faculty of Life and Environmental Sciences; Japón
Fil: Rothan, Christophe. Institut National de la Recherche Agronomique; Francia
Fuente
Journal of Experimental Botany 72 (4) : 1181-1197 (Febrero 2021)
Materia
Arabidopsis thaliana
Brasinoesteroides
Fruto
Crecimiento
Solanum lycopersicum
Transcripción
Brassinosteroids
Fruit
Growth
Transcription
Tomatoes
Cell Expansion
Tomate
Expansión Celular
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10138
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10138
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spelling The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomatoMori, KentaroLemaire-Chamley, MartineJorly, JoanaCarrari, FernandoConte, MarianaAsamizu, ErikaMizoguchi, TsuyoshiEzura, HiroshiRothan, ChristopheArabidopsis thalianaBrasinoesteroidesFrutoCrecimientoSolanum lycopersicumTranscripciónBrassinosteroidsFruitGrowthTranscriptionTomatoesCell ExpansionTomateExpansión CelularBrassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling.Instituto de BiotecnologíaFil: Mori, Kentaro. Institut National de la Recherche Agronomique; FranciaFil: Lemaire-Chamley, Martine. Institut National de la Recherche Agronomique; FranciaFil: Jorly, Joana. Institut National de la Recherche Agronomique; FranciaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Conte, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asamizu, Erika. Ryukoku University. Faculty of Agriculture. Department of Plant Life Science; JapónFil: Mizoguchi, Tsuyoshi. International Christian University. Department of Natural Sciences; JapónFil: Ezura, Hiroshi. University of Tsukuba. Tsukuba Plant Innovation Research Center. Faculty of Life and Environmental Sciences; JapónFil: Rothan, Christophe. Institut National de la Recherche Agronomique; FranciaOxford University Press2021-08-30T11:34:32Z2021-08-30T11:34:32Z2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10138https://academic.oup.com/jxb/article-abstract/72/4/1181/59372261460-2431https://doi.org/10.1093/jxb/eraa495Journal of Experimental Botany 72 (4) : 1181-1197 (Febrero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:19Zoai:localhost:20.500.12123/10138instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:20.058INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
title The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
spellingShingle The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
Mori, Kentaro
Arabidopsis thaliana
Brasinoesteroides
Fruto
Crecimiento
Solanum lycopersicum
Transcripción
Brassinosteroids
Fruit
Growth
Transcription
Tomatoes
Cell Expansion
Tomate
Expansión Celular
title_short The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
title_full The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
title_fullStr The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
title_full_unstemmed The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
title_sort The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
dc.creator.none.fl_str_mv Mori, Kentaro
Lemaire-Chamley, Martine
Jorly, Joana
Carrari, Fernando
Conte, Mariana
Asamizu, Erika
Mizoguchi, Tsuyoshi
Ezura, Hiroshi
Rothan, Christophe
author Mori, Kentaro
author_facet Mori, Kentaro
Lemaire-Chamley, Martine
Jorly, Joana
Carrari, Fernando
Conte, Mariana
Asamizu, Erika
Mizoguchi, Tsuyoshi
Ezura, Hiroshi
Rothan, Christophe
author_role author
author2 Lemaire-Chamley, Martine
Jorly, Joana
Carrari, Fernando
Conte, Mariana
Asamizu, Erika
Mizoguchi, Tsuyoshi
Ezura, Hiroshi
Rothan, Christophe
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Brasinoesteroides
Fruto
Crecimiento
Solanum lycopersicum
Transcripción
Brassinosteroids
Fruit
Growth
Transcription
Tomatoes
Cell Expansion
Tomate
Expansión Celular
topic Arabidopsis thaliana
Brasinoesteroides
Fruto
Crecimiento
Solanum lycopersicum
Transcripción
Brassinosteroids
Fruit
Growth
Transcription
Tomatoes
Cell Expansion
Tomate
Expansión Celular
dc.description.none.fl_txt_mv Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling.
Instituto de Biotecnología
Fil: Mori, Kentaro. Institut National de la Recherche Agronomique; Francia
Fil: Lemaire-Chamley, Martine. Institut National de la Recherche Agronomique; Francia
Fil: Jorly, Joana. Institut National de la Recherche Agronomique; Francia
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Conte, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asamizu, Erika. Ryukoku University. Faculty of Agriculture. Department of Plant Life Science; Japón
Fil: Mizoguchi, Tsuyoshi. International Christian University. Department of Natural Sciences; Japón
Fil: Ezura, Hiroshi. University of Tsukuba. Tsukuba Plant Innovation Research Center. Faculty of Life and Environmental Sciences; Japón
Fil: Rothan, Christophe. Institut National de la Recherche Agronomique; Francia
description Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-30T11:34:32Z
2021-08-30T11:34:32Z
2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10138
https://academic.oup.com/jxb/article-abstract/72/4/1181/5937226
1460-2431
https://doi.org/10.1093/jxb/eraa495
url http://hdl.handle.net/20.500.12123/10138
https://academic.oup.com/jxb/article-abstract/72/4/1181/5937226
https://doi.org/10.1093/jxb/eraa495
identifier_str_mv 1460-2431
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Journal of Experimental Botany 72 (4) : 1181-1197 (Febrero 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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